Abstract
Sausage is an emulsified meat product that, due to its composition, undergoes physicochemical changes during its shelf life, which makes the use of additives for its conservation necessary. The goal of the present study was to evaluate the antioxidant capacity of rosemary extract, acerola extract, and a mixture of tocopherols applied to industrialized sausages during storage at 8 °C. The antioxidant activity (IC50) in vitro showed values of 0.043, 0.489, 0.494, and 0.509 mg/mL for sodium erythorbate, rosemary extract, acerola extract, and a mixture of tocopherols, respectively. Formulations of sausage obtained in industrial installations were evaluated in terms of physicochemical, microbiological, and sensorial analyses. The pH and acidity values were stable during 23 days of storage. Treatments 1 and 3 with acerola extract and a mixture of tocopherols associated with sodium erythorbate showed the best results against lipid oxidation (TBARs), respectively. The hydroperoxides were only found after the 12th day of storage, consequently reducing the formation of malondialdehyde. The treatments with natural antioxidants showed an antimicrobial effect for the group of mesophilic bacteria; their results did not exceed 4 log10 CFU/g, while the control reached 5 log10 CFU/g on the 23rd day of storage. In regard to other microorganisms evaluated, no significant differences were found between treatments with natural antioxidants. Thus, the natural extracts evaluated in association with sodium erythorbate contributed to the antioxidant action for the application on an industrial scale, as they improved the sausage characteristics after 23 days of storage at 8 °C.
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Alvarado-Martinez Z, Aditya A, Biswas D (2020) Chapter 14—plant antioxidants, extraction strategies, and their application in meat. In: Biswas AK, Mandal PK (eds) Meat quality analysis. Academic Press, Cambridge, pp 241–264. https://doi.org/10.1016/B978-0-12-819233-7.00014-8
Álvarez D, Xiong YL, Castillo M, Payne FA, Garrido MD (2012) Textural and viscoelastic properties of pork frankfurters containing canola–olive oils, rice bran, and walnut. Meat Sci 92(1):8–15. https://doi.org/10.1016/j.meatsci.2012.03.012
Azab DE-SH, Almoselhy RIM, Mahmoud MH (2022) Improving the quality characteristics of low fat toffee by using mango kernel fat, pectin, and high-speed homogenizer. J Food Process Preserv 46(12):e17235. https://doi.org/10.1111/jfpp.17235
Bianchin M, Pereira D, de Almeida JF, de Moura C, Pinheiro RS, Heldt LFS, Haminiuk CWI, Carpes ST (2020) Antioxidant properties of lyophilized rosemary and sage extracts and its effect to prevent lipid oxidation in poultry pátê. Molecules 25(21):5160. https://doi.org/10.3390/molecules25215160
Brasil (2000) Ministério da Agricultura, Pecuária e do Abastecimento. Secretaria de Defesa Agropecuária. Instrução Normativa no 22, de 31 de julho de 2000. Aprova o Regulamento Técnico de Identidade de Qualidade de Salames
Brazil (2019) Instrução Normativa no 60 de 23 de dezembro de 2019. Listas de padrões microbiológicos para alimentos. Diário Oficial da União de 26/12/2019
Campo MM, Nute GR, Hughes SI, Enser M, Wood JD, Richardson RI (2006) Flavour perception of oxidation in beef. Meat Sci 72(2):303–311. https://doi.org/10.1016/j.meatsci.2005.07.015
Cropotova J, Mozuraityte R, Standal IB, Rustad T (2019) Assessment of lipid oxidation in Atlantic mackerel (Scomber scombrus) subjected to different antioxidant and sous-vide cooking treatments by conventional and fluorescence microscopy methods. Food Control 104:1–8. https://doi.org/10.1016/j.foodcont.2019.04.016
de Garcia-Santos MSL, Conceição FS, Villas Boas F, Maria SB, da Barreto ACS (2019) Effect of the addition of resistant starch in sausage with fat reduction on the physicochemical and sensory properties. Food Sci Technol 39(suppl 2):491–497. https://doi.org/10.1590/fst.18918
de Matos Junior FE, Thomazoni M, Trindade MA, Fávaro-Trindade CS (2015) Application of free or encapsulated vitamin C to chicken frankfurter sausage by spray chilling: physicochemical characteristics, stability and sensory acceptance. Braz J Food Technol 18(4):322–331. https://doi.org/10.1590/1981-6723.5615
de Paiva GB, Trindade MA, Romero JT, da Silva-Barretto AC (2021) Antioxidant effect of acerola fruit powder, rosemary and licorice extract in caiman meat nuggets containing mechanically separated caiman meat. Meat Sci 173:108406. https://doi.org/10.1016/j.meatsci.2020.108406
Dutcosky SD (2013) Análise Sensorial de Alimentos, 4th edn. Champagnat –Pucpress
EFSA (2008) Scientific opinion of the panel on food additives, flavourings, processing aids and materials in contact with food on a request from the Commission on the use of rosemary extracts as a food additive. EFSA J 721:1–29
Estévez M (2011) Protein carbonyls in meat systems: a review. Meat Sci 89(3):259–279. https://doi.org/10.1016/j.meatsci.2011.04.025
FAO (2020) World food and agriculture—statistical yearbook 2020. FAO. https://doi.org/10.4060/cb1329en
Feng C-H, Sun D-W, García Martín JF, Zhang Z-H (2013) Effects of different cooling methods on shelf-life of cooked jumbo plain sausages. LWT Food Sci Technol 54(2):426–433. https://doi.org/10.1016/j.lwt.2013.05.033
Feng X, Fu C, Yang H (2017) Gelatin addition improves the nutrient retention, texture and mass transfer of fish balls without altering their nanostructure during boiling. LWT 77:142–151. https://doi.org/10.1016/j.lwt.2016.11.024
Firsov AM, Franco MSF, Chistyakov DV, Goriainov SV, Sergeeva MG, Kotova EA, Fomich MA, Bekish AV, Sharko OL, Shmanai VV, Itri R, Baptista MS, Antonenko YN, Shchepinov MS (2022) Deuterated polyunsaturated fatty acids inhibit photoirradiation-induced lipid peroxidation in lipid bilayers. J Photochem Photobiol B Biol 229:112425. https://doi.org/10.1016/j.jphotobiol.2022.112425
Gadekar YP, Sharma BD, Shinde AK, Verma AK, Mendiratta SK (2014) Effect of natural antioxidants on the quality of cured, restructured goat meat product during refrigerated storage (4 ± 1°C). Small Rumin Res 119(1):72–80. https://doi.org/10.1016/j.smallrumres.2014.03.005
Gänzle MG (2015) Lactic metabolism revisited: metabolism of lactic acid bacteria in food fermentations and food spoilage. Curr Opin Food Sci 2:106–117. https://doi.org/10.1016/j.cofs.2015.03.001
Harlina PW, Ma M, Shahzad R, Khalifa I (2022) Effect of rosemary extract on lipid oxidation, fatty acid composition, antioxidant capacity, and volatile compounds of salted duck eggs. Food Sci Anim Resour 42(4):689–711. https://doi.org/10.5851/kosfa.2022.e30
Holck A, Axelsson L, McLeod A, Rode T, Heir E (2017) Health and safety considerations of fermented sausages. J Food Qual 2017:1–25. https://doi.org/10.1155/2017/9753894
IAL (2008) Instituto Adolfo Lutz. Métodos físico-químicos para análise de alimentos. In: Métodos físicos-quimicos para análise de Alimentos. https://doi.org/10.1017/CBO9781107415324.004
Kalache R, Matiucci MA, de Marins AR, de Campos TAF, Feihrmann AC (2021) High pressure application in sausage preservation. Braz J Dev 7(2):13208–13218. https://doi.org/10.34117/bjdv7n2-100
Karpińska-Tymoszczyk M (2013) The effect of oil-soluble rosemary extract, sodium erythorbate, their mixture, and packaging method on the quality of Turkey meatballs. J Food Sci Technol 50(3):443–454. https://doi.org/10.1007/s13197-011-0359-3
Kumar Y, Yadav DN, Ahmad T, Narsaiah K (2015) Recent trends in the use of natural antioxidants for meat and meat products. Compr Rev Food Sci Food Saf 14(6):796–812. https://doi.org/10.1111/1541-4337.12156
Lee HJ, Yong HI, Kim M, Choi Y-S, Jo C (2020) Status of meat alternatives and their potential role in the future meat market—a review. Asian Australas J Anim Sci 33(10):1533–1543. https://doi.org/10.5713/ajas.20.0419
Levine H, Finley J (2018) Texture, pp 329–363. https://doi.org/10.1007/978-3-319-63607-8_8
Liu D-C, Tsau R-T, Lin Y-C, Jan S-S, Tan F-J (2009) Effect of various levels of rosemary or Chinese mahogany on the quality of fresh chicken sausage during refrigerated storage. Food Chem 117(1):106–113. https://doi.org/10.1016/j.foodchem.2009.03.083
Lorenzetti E, Soares J, Treichel H, Cansian RL, Steffens C, Valduga E (2015) Brine absorption in seasoned chicken pieces. J Fur Verbraucherschutz Und Lebensmittelsicherheit. https://doi.org/10.1007/s00003-015-0946-8
Meats and Sausages (2022) Materials and ingredients. www.meatsandsausages.com/sausage-types/vegetarian/ingredients
Miranda A, Fraga C (2006) Free radical scavenger activity: determination of the antioxidant profile of bioactive substances. In: Practical studies for medicinal chemistry
Novaković S, Tomašević I (2017) A comparison between Warner-Bratzler shear force measurement and texture profile analysis of meat and meat products: a review. IOP Conf Ser Earth Environ Sci 85:012063. https://doi.org/10.1088/1755-1315/85/1/012063
OECD, Food, & of the United Nations AO (2022) OECD-FAO agricultural outlook 2022–2031. https://doi.org/10.1787/f1b0b29c-en
Paredes J, Cortizo-Lacalle D, Imaz AM, Aldazabal J, Vila M (2022) Application of texture analysis methods for the characterization of cultured meat. Sci Rep 12(1):3898. https://doi.org/10.1038/s41598-022-07785-1
Paula R, Colet R, de Oliveira D, Valduga E, Treichel H (2011) Assessment of different packaging structures in the stability of frozen fresh Brazilian Toscana sausage. Food Bioprocess Technol 4(3):481–485. https://doi.org/10.1007/s11947-010-0350-4
Raharjo S, Sofos JN, Schmidt GR (1992) Improved speed, specificity, and limit of determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid peroxidation in beef. J Agric Food Chem 40(11):2182–2185. https://doi.org/10.1021/jf00023a027
Realini CE, Guàrdia MD, Díaz I, García-Regueiro JA, Arnau J (2015) Effects of acerola fruit extract on sensory and shelf-life of salted beef patties from grinds differing in fatty acid composition. Meat Sci 99:18–24. https://doi.org/10.1016/j.meatsci.2014.08.008
Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin (2004)
Rocío Teruel M, Garrido MD, Espinosa MC, Linares MB (2015) Effect of different format-solvent rosemary extracts (Rosmarinus officinalis) on frozen chicken nuggets quality. Food Chem 172:40–46. https://doi.org/10.1016/j.foodchem.2014.09.018
Sampaio GR, Saldanha T, Soares RAM, Torres EAFS (2012) Effect of natural antioxidant combinations on lipid oxidation in cooked chicken meat during refrigerated storage. Food Chem 135(3):1383–1390. https://doi.org/10.1016/j.foodchem.2012.05.103
Schwert R, Verlindo R, Soares JM, Silva PF, Cansian RL, Steffens C, Treichel H, Valduga E (2020) Effect of liquid smoke extract on the oxidative stability, benzopyrene and sensory quality of calabrese sausage. Curr Nutr Food Sci 16(3):343–353. https://doi.org/10.2174/1573401315666190126120749
Sebranek JG, Sewalt VJH, Robbins KL, Houser TA (2005) Comparison of a natural rosemary extract and BHA/BHT for relative antioxidant effectiveness in pork sausage. Meat Sci 69(2):289–296. https://doi.org/10.1016/j.meatsci.2004.07.010
Solís-Calero C, Ortega-Castro J, Frau J, Muñoz F (2015) Nonenzymatic reactions above phospholipid surfaces of biological membranes: reactivity of phospholipids and their oxidation derivatives. Oxidative Med Cell Longev 2015:319505. https://doi.org/10.1155/2015/319505
Souri E, Amin G, Farsam H, Barazandeh Tehrani M (2008) Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts. Daru 16:83–87
Teixeira BF, Aranha JB, de Souza Vieira TMF (2022) Replacing synthetic antioxidants in food emulsions with microparticles from green acerola (Malpighia emarginata). Future Foods 5:100130. https://doi.org/10.1016/j.fufo.2022.100130
Villalobos-Delgado LH, Mateo J, Caro I, Leal Ramos M-Y, Mendez NG, Cansino RG, González Mondragón EG (2019) Chapter 11—natural antioxidants in fresh and processed meat. In: Galanakis CM (ed) Sustainable meat production and processing. Academic Press, Cambridge, pp 207–236. https://doi.org/10.1016/B978-0-12-814874-7.00011-0
Wang B, Pace RD, Dessai AP, Bovell-Benjamin A, Phillips B (2002) Modified extraction method for determining 2-thiobarbituric acid values in meat with increased specificity and simplicity. J Food Sci 67(8):2833–2836. https://doi.org/10.1111/j.1365-2621.2002.tb08824.x
Yuan X, Zhu X, Sun R, Jiang W, Zhang D, Liu H, Sun B (2022) Sensory attributes and characterization of aroma profiles of fermented sausages based on fibrous-like meat substitute from soybean protein and Coprinus comatus. Food Chem 373:131537. https://doi.org/10.1016/j.foodchem.2021.131537
Žegura B, Dobnik D, Niderl MH, Filipič M (2011) Antioxidant and antigenotoxic effects of rosemary (Rosmarinus officinalis L.) extracts in Salmonella typhimurium TA98 and HepG2 cells. Environ Toxicol Pharmacol 32(2):296–305. https://doi.org/10.1016/j.etap.2011.06.002
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The authors would like to thank the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel Brazil (CAPES)—Finance Code 001, the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (Fapergs), and the URI for the financial and structure support.
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Hoelscher, H., Fell, E.L., Colet, R. et al. Antioxidant activity of rosemary extract, acerola extract and a mixture of tocopherols in sausage during storage at 8 °C. J Food Sci Technol 61, 69–83 (2024). https://doi.org/10.1007/s13197-023-05815-y
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DOI: https://doi.org/10.1007/s13197-023-05815-y