Skip to main content

Advertisement

SpringerLink
Log in

Eugenol nanoemulsion as bio-fumigant: enhanced insecticidal activity against the rice weevil, Sitophilus oryzae adults

  • ORIGINAL ARTICLE
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Nanoemulsion is a promising delivery system for delivering the plant bioactive molecules against insect pests. In this study, we aimed to prepare eugenol based nanoemulsions (EL-NE) by ultrasonication method to investigate its fumigant toxicity against Sitophilus oryzae adults and to analyse the residual characteristics of eugenol bioactive on the treated grains and beetles. In EL-NE preparations, 1:1 ratio of eugenol: Tween 80 combination with 5 min of ultrasonication at frequency of 10 kHz and 12 W power output was determined as optimal. In the optimized nanoemulsions, 19.21 to 42.82 d.nm range of mean droplet size, 0.50 to 0.77 range of polydispersity index and −21.80 to −29.83 mV range of zeta potential values were observed with respect to 2.5 to 10.0% of eugenol concentrations. After 72 h of fumigation, enhanced fumigant toxicities (3.5–11.2 fold) were observed against S. oryzae adults for the optimized EL-NEs compared to eugenol alone. Fumigant toxicity results revealed 14.40 µl/L air of least LC50 value for the 10.0% EL-NE. Persistence of eugenol was more (12.46%) in EL-NE treated wheat grains compared to eugenol alone treatments based on Gas Chromatography–Mass Spectroscopy analysis, which indicates the improved fumigation. This study results suggests EL-NEs as promising nano-biofumigant against the S. oryzae adults for eco-friendly Integrated Pest Management (IPM).

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

All data generated or analysed during this study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

References

  • Abouelkassem S, Abdelrazeik AB, Rakha OM (2015) Nanoemulsion of Jojoba oil, preparation, characterization and insecticidal activity against Sitophilus oryzae (Coleoptera: Curculionidae) on wheat. Int J Agric Innov Res 4:1473–2319

    Google Scholar 

  • Adak T, Barik N, Patil NB, Govindharaj GPP, Gadratagi BG, Annamalai M, Mukherjee AK, Rath PC (2019) Nanoemulsion of Eucalyptus oil: an alternative to synthetic pesticides against two major storage insects Sitophilus oryzae and Tribolium castaneum (Herbst) of rice. Ind Crops Prod 143:111849

    Article  Google Scholar 

  • Ahmad N, Alam MA, Ahmad FJ, Sarafroz M, Ansari K, Sharma S, Amir M (2018) Ultrasonication techniques used for the preparation of novel eugenol-nanoemulsion in the treatment of wounds healings and anti-inflammatory. J Drug Deliv Sci Technol 46:461–473

    Article  CAS  Google Scholar 

  • Ajayi OE, Appel AG, Fadamiro HY (2014) Fumigation toxicity of essential oil monoterpenes to Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae). J Insects 2014:1–7

    Article  Google Scholar 

  • Anjali CH, Sharma Y, Mukherjee A, Chandrasekaran N (2012) Neem oil (Azadirachta indica) nanoemulsion—a potent larvicidal agent against Culex quinquefasciatus. Pest Manag Sci 68:158–163

    Article  CAS  PubMed  Google Scholar 

  • Armijos MJG, Jumbo LOV, Faroni LRDA, Oliveira EE, Flores AF, Heleno FF, Haddi K (2019) Fumigant toxicity of eugenol and its negative effects on biological development of Callosobruchus maculatus. Rev Cienc Agríc 36(1):5–15

    Google Scholar 

  • Cáceres M, Guzmán E, Alvarez-Costa A, Ortega F, Rubio RG, Coviella C, Orihuela PLS, Vassena CV, Lucia A (2020) Surfactantless emulsions containing eugenol for imidacloprid solubilization: physicochemical characterization and toxicity against insecticide-resistant Cimex lectularius. Molecules 25:2290

    Article  PubMed  PubMed Central  Google Scholar 

  • Chuacharoen T, Sabliov CM (2016) Stability and controlled release of lutein loaded in zein nanoparticles with and without lecithin and pluronic F127 surfactants. Colloids Surf A Physicochem Eng Asp 503:11–18

    Article  CAS  Google Scholar 

  • CIBRC (2021) List of pesticides which are banned, refused registration and restricted use. http://ppqs.gov.in/divisions/cib-rc/registered-products. Accessed on 8th Feb 2022

  • Dimetry NZ, El-Wakeil N, Hussein H (2019) Essential oils development through nanoparticles for managing stored product insect pests. Trends Appl Sci Res 14(3):142–159

    Article  CAS  Google Scholar 

  • EFSA (2012) Conclusion on the peer review of the pesticide risk assessment of the active substance eugenol. EFSA J 10:2506

    Article  Google Scholar 

  • El-Wakeil NE (2013) Botanical pesticides and their mode of action. Gesunde Pflanzen 65(4):125–149

    Article  CAS  Google Scholar 

  • Gooderham NJ, Cohen SM, Eisenbrand G, Fukushima S, Guengerich FP, Hecht SS, Rietjends IMCM, Rosol TJ, Davidsen JM, Harman CL, Murray IJ, Taylor SV (2020) FEMA GRAS assessment of natural flavor complexes: clove, cinnamon leaf and West Indian Bay leaf-derived flavoring ingredients. Food Chem Toxicol 145:111585

    Article  CAS  PubMed  Google Scholar 

  • Kalaitzaki A, Papanikolaou NE, Karamaouna F, Dourtoglou V, Xenakis A, Papadimitriou V (2015) Biocompatible colloidal dispersions as potential formulations of natural pyrethrins: a structural and efficacy study. Langmuir 31:5722–5730

    Article  CAS  PubMed  Google Scholar 

  • Kanda D, Kaur S, Koul O (2017) A comparative study of monoterpenoids and phenylpropanoids from essential oils against stored grain insects: acute toxins or feeding deterrents. J Pest Sci 90:531–545

    Article  Google Scholar 

  • Karthik P, Anandharamakrishnan C (2016a) Fabrication of a nutrient delivery system of docosahexaenoic acid nanoemulsions via high energy techniques. RSC Adv 6:3501–3513

    Article  CAS  Google Scholar 

  • Karthik P, Anandharamakrishnan C (2016b) Enhancing omega-3 fatty acids nanoemulsion stability and in-vitro digestibility through emulsifiers. J Food Eng 187:92–105

    Article  CAS  Google Scholar 

  • Karthik P, Ezhilarasi PN, Anandharamakrishnan C (2017) Challenges associated in stability of food grade nanoemulsions. Crit Rev Food Sci Nutr 57(7):1435–1450

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Sihag P, Trotta F, Rao R (2018) Encapsulation of babchi oil in cyclodextrin-based nanosponges: physicochemical characterization, photodegradation, and in vitro cytotoxicity studies. Pharmaceutics 10:169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lucia A, Toloza AC, Fanucce M, Fernández-Peña L, Ortega F, Rubio RG, Coviella C, Guzmán E (2020) Nanoemulsions based on thymol-eugenol mixtures: characterization, stability and larvicidal activity against Aedes aegypti. Bull Insectology 73(1):153–160

    Google Scholar 

  • Mahran HA (2022) Using nanoemulsions of the essential oils of a selection of medicinal plants from Jazan, Saudi Arabia, as a green larvicidal against Culex pipiens. PLoS One 17(5):e0267150

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • McClements DJ (2012) Nanoemulsions versus microemulsions: terminology, differences, and similarities. Soft Matter 8:1719–1729

    Article  CAS  Google Scholar 

  • Nguyen TT, Collins PJ, Ebert PR (2015) Inheritance and characterization of strong resistance to phosphine in Sitophilus oryzae (L.). PloS One 10(4):e0124335

    Article  PubMed  PubMed Central  Google Scholar 

  • Nielloud F, Marti-Mestres G (2000) Pharmaceutical emulsions and suspensions. Marcel Dekker Inc, New York

    Book  Google Scholar 

  • Nuruzzaman Md, Rahman MM, Liu Y, Naidu R (2016) Nanoencapsulation, nano-guard for pesticides: a new window for safe application. J Agric Food Chem 64:1447–1483

    Article  CAS  PubMed  Google Scholar 

  • Pongsumpun P, Iwamoto S, Siripatrawan U (2020) Response surface methodology for optimization of cinnamon essential oil nanoemulsion with improved stability and antifungal activity. Ultrason Sonochem 60:104604

    Article  CAS  PubMed  Google Scholar 

  • Raja MRC, Srinivasan V, Selvaraj S, Mahapatra SK (2015) Versatile and synergistic potential of eugenol: a review. Pharm Anal Acta 6(5):1–6

    Google Scholar 

  • Reddy DN (2019) Essential oils extracted from medicinal plants and their applications. In: Akhtar MS et al. (Eds.) Natural Bio-Active Compounds, Springer Nature Singapore Pte Ltd. 237–283

  • Sahu U, Ibrahim SS, Vendan SE (2021) Persistence and ingestion characteristics of phytochemical volatiles as bio-fumigants in Sitophilus oryzae adults. Ecotoxicol Environ Saf 210:111877

    Article  CAS  PubMed  Google Scholar 

  • Shinoda K, Saito H (1969) The stability of O/W type emulsions as functions of temperature and the HLB of emulsifiers: the emulsification by PIT-method. J Colloid Interface Sci 30:258–263

    Article  CAS  Google Scholar 

  • Shishir MRI, Xie L, Sunb C, Zheng X, Chen W (2018) Advances in micro and nano-encapsulation of bioactive compounds using biopolymer and lipid-based transporters. Trends Food Sci Technol 78:34–60

    Article  CAS  Google Scholar 

  • Sun YP (1950) Toxicity index an improved method of comparing the relative toxicity of insecticides. J Econ Entomol 43:45–53

    Article  CAS  Google Scholar 

  • Turek C, Stintzing FC (2013) Stability of essential oils: a review. Compr Rev Food Sci Food Saf 12:40–53

    Article  CAS  Google Scholar 

  • Vendan SE, Manivannan S, Sunny AM, Murugesan R (2017) Phytochemical residue profiles in rice grains fumigated with essential oils for the control of rice weevil. PLoS One 12(10):e0186020

    Article  PubMed  PubMed Central  Google Scholar 

  • Vilasau J, Solans C, Gómez MJ, Dabrio J, Mújika-Garai R, Esquena J (2011) Phase behaviour of a mixed ionic/nonionic surfactant system used to prepare stable oil-in-water paraffin emulsions. Colloids Surf A Physicochem Eng Asp 384:473–481

    Article  CAS  Google Scholar 

  • Wang Z, Neves MA, Yin LJ, Kobayashi I, Uemura K, Nakajima M (2012) In vitro gastrointestinal digestibility of soybean oil-in-water emulsion droplets stabilized by polyglycerol esters of fatty acid. Food Sci Technol Res 18(2):149–156

    Article  CAS  Google Scholar 

  • Wilson JA, Isman MB (2006) Influence of essential oils on toxicity and pharmacokinetics of the plant toxin thymol in the larvae of Trichoplusia ni. Can Entomol 138:578–589

    Article  Google Scholar 

Download references

Acknowledgements

All authors are grateful to the Director, CSIR-CFTRI, Mysore for providing the facilities and support for this study. We also sincerely acknowledge Dr. P. Arun Kumar (Principal Scientist, FPT, CSIR-CFTRI, Mysore) for his valuable review comments in this manuscript. Sincere thanks to Mr. B. Eswaran and Mr. K. Narayanan for their technical support for GC-MS and DLS instrument handling and analysis, respectively.

Funding

This study was conducted under the Council of Scientific and Industrial Research – The World Academy of Sciences (CSIR-TWAS) postdoctoral fellowship programme. First author is the recipient of this fellowship grant (FR No. 3240306313).

Author information

Author notes

  1. Samar Sayed Ibrahim

    Present address: Pests and Plant Protection Department, National Research Centre, Cairo, 12622, Egypt

Authors and Affiliations

  1. Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India

    Samar Sayed Ibrahim, Urvashi Sahu & Subramanian Ezhil Vendan

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201 002, India

    Urvashi Sahu & Subramanian Ezhil Vendan

  3. Department of Food Technology, Faculty of Engineering, Karpagam Academy of Higher Education (Deemed to Be University), Coimbatore, 641 021, India

    Pothiyappan Karthik

Authors
  1. Samar Sayed Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Urvashi Sahu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pothiyappan Karthik
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Subramanian Ezhil Vendan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

SS and EV conceived and designed research. SS and US conducted experiments. SS, EV and PK analysed data. SS, US, PK and EV wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Subramanian Ezhil Vendan.

Ethics declarations

Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical approval

Not applicable.

Consent to participate

The authors declare that they have agreed for participation during the research study, manuscript preparation and processing.

Consent for publication

All authors declare that they have complete consent for publishing the data and manuscript in the journal.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ibrahim, S.S., Sahu, U., Karthik, P. et al. Eugenol nanoemulsion as bio-fumigant: enhanced insecticidal activity against the rice weevil, Sitophilus oryzae adults. J Food Sci Technol 60, 1435–1445 (2023). https://doi.org/10.1007/s13197-023-05690-7

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-023-05690-7

Keywords

Search

Navigation