Abstract
Litchi fruit is consumed across the globe for its high nutritional value and taste. The qualitative profiling of litchi fruit has been carried out by using ultra-high-performance liquid chromatography with QExactive high-resolution accurate mass spectrometry. Acidified water: methanol: acetonitrile (1:1:1) extracts from individual parts (skin, pulp, and seed) of matured litchi, were subjected to LC–MS analysis with electrospray ionization in full MS-ddMS2 mode as a non-target approach. The data was processed through compound discoverer software by the use of mzCloud and ChemSpider databases, for compound identification. We identified 77 compounds with protonated or deprotonated forms based on the polarity and their characteristic fragments are within ± 4 ppm mass error and retention time ± 0.1 min for parent and fragments. Hypoglycin B is the first time reported in litchi fruit along with hypoglycin A. Further, we verified the distribution of the identified components and differentiation of three different parts of litchi through principal component analysis.
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All the data provided in manuscript is correct and also available with us if required. The datasets generated and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- LC-MS:
-
Liquid chromatography mass spectrometer
- MCPG:
-
Methylenecyclopropyl-glycine
- UHPLC:
-
Ultra high performance liquid chromatography
- HCA:
-
Hierarchical cluster analysis
- PCA:
-
Principal component analysis
- AGC:
-
Automatic gain control
- HESI:
-
Heated electrospray ionization
- TIC:
-
Total ion chromatogram
- ddMS2:
-
Data dependent MS2
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This work was supported by the FSSAI-Thermo Fisher Food Safety Solution Center (FSSC) and the FSSAI, New Delhi.
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Oulkar, D., Singh, K. & Narayan, B. Characterization of different parts of litchi fruit using UHPLC-QExactive Orbitrap. J Food Sci Technol 59, 4889–4906 (2022). https://doi.org/10.1007/s13197-022-05577-z
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DOI: https://doi.org/10.1007/s13197-022-05577-z