Abstract
The purpose of the current study was to examine the effect of adding secondary ingredients such as green tea derived water-soluble polysaccharides (GTP) and flavonol aglycone rich fractions derived from cellulase treated green tea extract (FVN) into catechin rich green tea extracts (GTE) on wheat starch digestion and intestinal glucose transport using in vitro digestion with Caco-2 cells. Co-digestion of wheat starch with GTE (16.88 g L−1) or GTE + GTP + FVN (16.69 g L−1) appeared to promote starch hydrolysis compared to control (15.49 g L−1). In case of major flavonoids, addition of epigallocatechin gallate (EGCG), EGCG + myricetin (M) into wheat starch significantly increased the digestion of starch into glucose. Glucose transport rate decreased by 22.35% in wheat starch + GTE + GTP + FVN (1.39%), while the least amount of glucose (1.70%) was transported in EGCG mixed with M (1% of EGCG) as secondary ingredients among individual flavonoids formulation. It indicated that inhibitory effect on glucose transport was higher in addition of GTE, GTP, and FVN as excipients ingredients rather than targeted major flavonoids. Results from the current study suggest that whole green tea including flavonoid rich fractions could enhance hypoglycemic potential of GTE.
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Abbreviations
- ANOVA:
-
Analysis of variance
- AQ:
-
Aqueous fraction from digesta
- CG:
-
Catechin gallate
- COMT:
-
Catechol-O-methyl transferase
- DPBS:
-
Dulbecco’s Phosphate Buffered Saline
- EC:
-
Epigallocatechin
- ECG:
-
Epicatechin gallate
- EGC:
-
Epigallocatechin
- EGCG:
-
Epigallocatechin gallate
- FVN:
-
Flavonol aglycone rich fractions derived from cellulase treated green tea extract
- GCG:
-
Gallocatechin gallate
- GLUT2:
-
Glucose transporter 2
- GTE:
-
Catechin rich green tea extracts
- GTP:
-
Green tea derived water-soluble polysaccharides
- HPLC:
-
High performance liquid chromatography
- K:
-
Kaempferol
- M:
-
Myricetin
- MRP:
-
Efflux transporters including multidrug resistance associated protein
- Q:
-
Quercetin
- RO:
-
Reverse osmosis
- SGLT1:
-
Sodium dependent glucose transporter 1
- v:
-
Volume
- v/v:
-
Volume fraction
- w/w:
-
Weight fraction
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This research was supported by the AMOREPACIFIC
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In this study, CJO, RCS, PMY, HYD, and SSM did conceptualize and supervise the experiment. LYE and YSH did in vitro study and formal analysis and writing original draft. LHJ, OJH did in vitro study. LYE and SSM did review & editing.
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Lee, YE., Yoo, SH., Chung, JO. et al. Impact of flavonol extracts derived from green tea or targeted flavonols as secondary ingredients on intestinal glucose transport. J Food Sci Technol 59, 1317–1325 (2022). https://doi.org/10.1007/s13197-021-05140-2
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DOI: https://doi.org/10.1007/s13197-021-05140-2