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Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils

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Abstract

Groundnut oil (GNO)/peanut oil is one of the agro-food products with great economic value and hence an attractive target for adulteration and mislabeling. Simple Sequence Repeats (SSR) are markers of choice for DNA fingerprinting studies as they exhibit high polymorphism due to variable number of repeats. Hence, this study was designed to evaluate and optimize a method for DNA isolation from groundnut oil and study the possibility of using the isolated DNA for molecular traceability using SSR markers. Four methods to isolate DNA from groundnut oil were evaluated. All the four methods were modified CTAB protocols, but differed in procedures for extraction, buffer compositions, amount of oil used and DNA carriers. For molecular traceability of oils, extraction and recovery of DNA from edible oil is a key step, especially in refined oils. A method that employed DNA enrichment prior to extraction with CTAB buffer yielded amplifiable DNA from cold pressed GNO, crude hexane extracted GNO and refined GNO. The optimized method for isolation of DNA from groundnut oil is simple, efficient, less costly and reproducible when compared to chromatography and spectroscopy based techniques.

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Availability of data and material

Entire data is available in the student academic repository and the laboratories where the experiment was conducted.

Abbreviations

GNO:

Groundnut oil

HCGNO:

Hexane extracted crude groundnut oil

CPGNO:

Cold pressed groundnut oil

CRGNO:

Commercially available refined groundnut oil

SSR:

Simple Sequence Repeats

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Acknowledgments

The authors acknowledge the support of the Indian Council of Agricultural Research (ICAR) and MFPI—Quality Control Laboratory and Institute of Biotechnology of Professor Jayashankar Telangana State Agricultural University, Hyderabad for all the logistical assistance, infrastructure facility provided to carry out the study.

Funding

The research was conducted as a part of M.Sc Student Research work, supported by Professor Jayashankar Telangana State Agricultural University, Hyderabad, India.

Author information

Authors and Affiliations

  1. Post Graduate & Research Centre, PJTS Agricultural University, Hyderabad, India

    Keotshepile Precious Bojang

  2. 2MFPI-Quality Control Laboratory, PJTS Agricultural University, Hyderabad, India

    Aparna Kuna

  3. Institute of Biotechnology, PJTS Agricultural University, Hyderabad, India

    Sreerangam N. C. V. L. Pushpavalli

  4. Departmentof Foods & Nutrition, College of Community Science, PJTS Agricultural University, Hyderabad, India

    Supta Sarkar

  5. 5Regional Rice and Sugarcane Research Centre, PJTS Agricultural University, Rudrur, India

    M. Sreedhar

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  1. Keotshepile Precious Bojang
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Contributions

KPB: Conduct of Experiments, Investigation of results and validation of data, Writing the draft manuscript. AK: Conceptualization of research work and administration, Validation, Supervision of work, Writing review and editing. SNCVLP: Methodology, Investigation, Formal analysis, Validation, Supervision of work, Reviewing and editing of draft. SS: Methodology, Investigation, Proof reading and reviewing. MS: Methodology, Formal analysis, Review and editing of draft.

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Correspondence to Keotshepile Precious Bojang.

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Bojang, K.P., Kuna, A., Pushpavalli, S.N.C.V.L. et al. Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils. J Food Sci Technol 58, 3561–3567 (2021). https://doi.org/10.1007/s13197-021-05079-4

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  • DOI: https://doi.org/10.1007/s13197-021-05079-4

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