Abstract
Hydrolyzed collagen from the defatted Asian sea bass (Lates calcarifer) (Asbs-HC) had high hydrophobic amino acids and imino acids. When fibroblast cell was treated with Asbs-HC, there was no cytotoxicity at any concentrations (25–1000 µg/mL). Asbs-HC at 1000 µg/mL exhibited the highest cell proliferation and cell migration (p < 0.05), indicating wound healing ability. Antioxidative activities of Asbs-HC at different concentrations were determined. ABTS radical scavenging activity (ABTS-RSA) and oxygen radical absorbance capacity (ORAC) increased when Asbs-HC levels augmented up to 1 mg/mL (p < 0.05). Decreased activities in scavenging DPPH radical and chelating metal were found at higher levels of Asbs-HC (0.5 and 1 mg/mL) (p < 0.05). Molecular weight (MW) of peptides in Asbs-HC ranged from 406 to 16,120 Da. Peptide containing MW of 406 Da rendered the highest scavenging activity towards ABTS radical. Thus, Asbs-HC could be applied as antioxidant, skin nourishment and wound healing agents for food/drink fortification.
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Acknowledgements
This research was supported by the Thailand Research Fund under the Royal Golden Jubilee Ph.D. Program to Lalita Chotphruethipong (PHD/0183/2560). Prince of Songkla University (Grant No. AGR6302013N) and Foreign collaborative program (Grant No. AGR6302073S) were also acknowledged.
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Chotphruethipong, L., Sukketsiri, W., Aluko, R.E. et al. Effect of hydrolyzed collagen from defatted Asian sea bass (Lates calcarifer) skin on fibroblast proliferation, migration and antioxidant activities. J Food Sci Technol 58, 541–551 (2021). https://doi.org/10.1007/s13197-020-04566-4
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DOI: https://doi.org/10.1007/s13197-020-04566-4