Abstract
The phenolics from grapefruit peel were obtained by ultrasound assisted extraction (UAE) and enzyme-assisted extraction (EAE). Extraction parameters were optimized using response surface methodology to maximize the yield of total phenolic content (TPC) and total flavonoid content (TFC). The optimized extracts from UAE and EAE were then compared with conventional solvent extraction for their TPC, TFC, antioxidant activity and phenolic composition. The best optimized conditions for UAE was obtained at 33.12 min extraction time, 71.11% amplitude and a solvent–solid (SS) ratio of 39.63 mL/g. The optimized parameters for EAE were 4.81 h extraction time, 0.9% enzyme concentration and 40 mL/g SS ratio. Similar values of experimental and predicted TPC and TFC at optimized conditions indicates the suitability of the quadratic model in optimizing the extraction parameters. Further characterization of extracts suggested EAE as most efficient process in extracting bioactive compounds.
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Apak R, Güçlü K, Özyürek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem 52(26):7970–7981
Arnous A, Meyer AS (2010) Discriminated release of phenolic substances from red wine grape skins (Vitis vinifera L.) by multicomponent enzymes treatment. Biochem Eng J 49(1):68–77
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239(1):70–76
Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28(1):25–30
Carrera C, Ruiz-Rodríguez C, Palma M, Barroso CG (2012) Ultrasound assisted extraction of phenolic compounds from grapes. Anal Chim Acta 732:100–104
Cho M, Ko SB, Kim JM, Lee OH, Lee DW, Kim JY (2016) Influence of extraction conditions on antioxidant activities and catechin content from bark of Ulmus pumila L. Appl Biol Chem 59(3):329–336
Dahmoune F, Boulekbache L, Moussi K, Aoun O, Spigno G, Madani K (2013) Valorization of Citrus limon residues for the recovery of antioxidants: evaluation and optimization of microwave and ultrasound application to solvent extraction. Ind Crops Prod 50:77–87
de Camargo AC, Regitano-d’Arce MAB, Biasoto ACT, Shahidi F (2016) Enzyme-assisted extraction of phenolics from winemaking by-products: antioxidant potential and inhibition of alpha-glucosidase and lipase activities. Food Chem 212:395–402
Garcia-Castello EM, Rodriguez-Lopez AD, Mayor L, Ballesteros R, Conidi C, Cassano A (2015) Optimization of conventional and ultrasound assisted extraction of flavonoids from grapefruit (Citrus paradisi L.) solid wastes. LWT Food Sci Technol 64(2):1114–1122
Hayat K, Hussain S, Abbas S, Farooq U, Ding B, Xia S, Jia C, Zhang X, Xia W (2009) Optimized microwave-assisted extraction of phenolic acids from citrus mandarin peels and evaluation of antioxidant activity in vitro. Sep Purif Technol 70(1):63–70
Hossain MB, Brunton NP, Patras A, Tiwari B, O’Donnell CP, Martin-Diana AB, Barry-Ryan C (2012) Optimization of ultrasound assisted extraction of antioxidant compounds from marjoram (Origanum majorana L.) using response surface methodology. Ultrason Sonochem 19:582–590
Imeh U, Khokhar S (2002) Distribution of conjugated and free phenols in fruits: antioxidant activity and cultivar variations. J Agric Food Chem 50(22):6301–6306
Kelebek H (2010) Sugars, organic acids, phenolic compositions and antioxidant activity of Grapefruit (Citrus paradisi) cultivars grown in Turkey. Ind Crops Prod 32:269–274
Kim DO, Lee CY (2002) Extraction and isolation of polyphenolics. In: Wrolstad RE (ed) Current protocols in food analytical chemistry. Wiley, New York, pp I1.2.1–I1.2.12
Li BB, Smith B, Hossain MM (2006) Extraction of phenolics from citrus peels: II. Enzyme-assisted extraction method. Sep Purif Technol 48(2):189–196
Libran CM, Mayor L, Garcia-Castello EM, Vidal-Brotons D (2013) Polyphenol extraction from grape wastes: solvent and pH effect. Agric Sci 4(9B):56–62
Londoño-Londoño J, de Lima VR, Lara O, Gil A, Pasa TBC, Arango GJ, Pineda JRR (2010) Clean recovery of antioxidant flavonoids from citrus peel: optimizing an aqueous ultrasound-assisted extraction method. Food Chem 119(1):81–87
Ma YQ, Chen JC, Liu DH, Ye XQ (2009) Simultaneous extraction of phenolic compounds of citrus peel extracts: effect of ultrasound. Ultrason Sonochem 16(1):57–62
Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79(5):727–747
Nayak B, Dahmoune F, Moussi K, Remini H, Dairi S, Aoun O, Khodir M (2015) Comparison of microwave, ultrasound and accelerated-assisted solvent extraction for recovery of polyphenols from Citrus sinensis peels. Food Chem 187:507–516
Oszmianski J, Lee CY (1990) Inhibitory effect of phenolics on carotene bleaching in vegetables. J Agric Food Chem 38(3):688–690
Padilla-Camberos E, Lazcano-Díaz E, Flores-Fernandez JM, Owolabi MS, Allen K, Villanueva-Rodríguez S (2014) Evaluation of the inhibition of carbohydrate hydrolyzing enzymes, the antioxidant activity, and the polyphenolic content of Citrus limetta peel extract. Sci World J 2014:121760
Pandey KB, Rizvi SI (2009) Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2(5):270–278
Ramic M, Vidovic S, Zekovic Z, Vladic J, Cvejin A, Pavlic B (2015) Modeling and optimization of ultrasound-assisted extraction of polyphenolic compounds from Aronia melanocarpa by-products from filter-tea factory. Ultrason Sonochem 23:360–368
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26(9–10):1231–1237
Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol 299:152–178
Spigno G, Tramelli L, De Faveri DM (2007) Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. J Food Eng 81(1):200–208
Wang Z, Shang Q, Wang W, Feng X (2011) Microwave-assisted extraction and liquid chromatography/mass spectrometry analysis of flavonoids from grapefruit peel. J Food Process Eng 34(3):844–859
Wilkins MR, Widmer WW, Grohmann K, Cameron RG (2007) Hydrolysis of grapefruit peel waste with cellulase and pectinase enzymes. Bioresour Technol 98(8):1596–1601
Williamson G, Plumb GW, Garcia-Conesa MT (1999) Glycosylation, esterification and polymerization of flavonoids and hydroxycinnamates: effects on antioxidant properties. Basic Life Sci 66:483–494
Xu G, Ye X, Chen J, Liu D (2007) Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract. J Agril Food Chem 55(2):330–335
Xu C, Yagiz Y, Borejsza-Wysocki W, Lu J, Gu L, Ramírez-Rodrigues MM, Marshall MR (2014) Enzyme release of phenolics from muscadine grape (Vitis rotundifolia Michx.) skins and seeds. Food Chem 157:20–29
Zhang S, Bi H, Liu C (2007) Extraction of bioactive components from Rhodiola sachalinensis under ultrahigh hydrostatic pressure. Sep Purif Technol 57(2):277–282
Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64(4):555–559
Zhou Z, Shao H, Han X, Wang K, Gong C, Yang X (2017) The extraction efficiency enhancement of polyphenols from Ulmus pumila L. barks by trienzyme-assisted extraction. Ind Crops Prod 97:401–408
Zhu Z, He J, Liu G, Barba FJ, Koubaa M, Ding L, Bals O, Grimi N, Vorobiev E (2016) Recent insights for the green recovery of inulin from plant food materials using non-conventional extraction technologies: a review. Innov Food Sci Emerg Technol 33:1–9
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The authors acknowledge the Division of Horticulture and Agricultural Chemicals, IARI, New Delhi, India, for providing fruits and research facilities.
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Nishad, J., Saha, S., Dubey, A.K. et al. Optimization and comparison of non-conventional extraction technologies for Citrus paradisi L. peels: a valorization approach. J Food Sci Technol 56, 1221–1233 (2019). https://doi.org/10.1007/s13197-019-03585-0
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DOI: https://doi.org/10.1007/s13197-019-03585-0