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Structural characterization and RAW264.7 murine macrophage stimulating activity of a fucogalactoglucan from Colpomenia peregrina

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Abstract

Water-soluble polysaccharides were isolated from Colpomenia peregrina to determine their chemical characteristics and immunomodulatory properties. High extraction yields were obtained for CP1 (17.6%) and CP2 (5.2%) polysaccharides. Polysaccharides were mainly consisted of neutral sugars (67.01–73.79%), uronic acids (9.43–14.89%), proteins (3.44–14.89%) and small amounts of sulfates (4.87–4.91%). Polysaccharides were composed of fucose (20.62–24.56%), galactose (25.5–26.94%) and glucose (50.00–52.91%) residues. The average molecular weights of the CP1 and CP2 polysaccharides were 1890 × 103 g/mol and 639 × 103 g/mol, respectively. The polysaccharides exerted a relatively low cytotoxicity against HeLa cancer cells (< 40%). The CP1 and CP2 polysaccharides were nontoxic and induced RAW264.7 murine macrophage cells to release considerable amounts of nitric oxide (NO). Inflammatory cytokines including IL-1β, TNF-α, IL-6, IL-10 and IL-12 from were secreted from RAW264.7 cells induced with CP1 polysaccharides. As the most immunostimulating fraction, CP1 polysaccharides were homogeneous and formed of 1,3-linked galactose, 1,4-linked glucose and 1,3-linked fucose residues. Overall, these findings suggested that the polysaccharides isolated from C. peregrina can be utilized as potential natural immunostimulant in functional foods or pharmaceutical industries.

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Authors and Affiliations

  1. Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, Iran

    Zeynab Rostami, Mehdi Tabarsa & Masoud Rezaei

  2. Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, Korea

    SangGuan You

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  1. Zeynab Rostami
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  2. Mehdi Tabarsa
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  3. SangGuan You
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  4. Masoud Rezaei
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Correspondence to Mehdi Tabarsa.

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Rostami, Z., Tabarsa, M., You, S. et al. Structural characterization and RAW264.7 murine macrophage stimulating activity of a fucogalactoglucan from Colpomenia peregrina. J Food Sci Technol 55, 4650–4660 (2018). https://doi.org/10.1007/s13197-018-3406-5

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  • DOI: https://doi.org/10.1007/s13197-018-3406-5

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