Abstract
This study was designed to evaluate antimicrobial activity and chemical composition of four different plant essential oils i.e. Ginger oil (GiO), Black seed oil (BSO), Oregano oil (OO) and Rose oil (RO) against different bacterial and fungal strains. Anti-microbial activities of selected essential oils were determined by the microbiological technique using Agar well diffusion assay. After in vitro study, most of the essential oils showed antimicrobial activity against all the selected pathogens. Among all the tested oils, GiO showed strong antimicrobial activity. GiO showed highest antimicrobial activity against Shigella (119.79%), Enteococcus hirae (110.61%) and Escherichia coli (106.02%), when compared with the tetracycline (50 µg/mL) activity. However, Antifungal activity of GiO was found to be present against Candida albicans and Aspergilluas flavus, when compared with clotrimazole (50 µg/mL) activity. Among all the selected bacteria, BSO showed maximum antimicrobial activity against the E. coli followed by Citrobacter freundii. Moreover, BSO had highest zone of inhibition against the C. ablicans (33.58%). OO indicated that, Shigella had the highest sensitivity (12.6 ± 0.58, 131.25%), followed by E. hirae (19.1 ± 0.61, 96.46%) and Salmonella typhi (15.2 ± 0.27, 83.06%) when compared with tetracycline activity. OO showed poor sensitivity against all the selected fungal strains. Furthermore, Gas Chromatography analysis revealed that, Gingerol (10.86%) was the chief chemical constituents found in GiO followed by α-Sesquiphellandrene (6.29%), Zingiberene (5.88%). While, BSO, OO and RO had higher percentage of p-Cymene (6.90%), Carvacrol (15.87%) and Citronellol (8.07%) respectively. The results exhibited that the essential oils used for this study was the richest source for antimicrobial activity which indicates the presence of broad spectrum antimicrobial compounds in these essential oils. Hence, essential oils and their components can be recommended for therapeutic purposes as source of an alternative medicine.
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Afef B, Hedia C, Maroua J, Abdennacer B, Mohamed B (2013) Essential oil composition and antibacterial activity of Origanum vulgare subsp, glandulosum desf, at different phenological stages. J Med Food 16:1115–1120
Ahmad I, Beg AZ (2001) Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 74:113–123
Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB (2001) Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem 49:4168–4170
Amel AS, Fadwa ME, Smah AS, Nazar AO (2015) Antimicrobial Activity of Zingiber officinale (Ginger) oil against bacteria isolated from children throat. Int J Microbiol 1:01–06
Babili FE, Bouajila J, Souchard JP, Bertrand C, Bellvert F, Fouraste I, Moulis C, Valentin A (2011) Oregano: chemical analysis and evaluation of its antimalarial, antioxidant and cytotoxic activities. J Food Sci 76:C512–C518
Balkan CE, Karamese M, Celebi D, Aydogdu S, Dicle Y, Calık Z (2016) The determination of the antibacterial activities of Rose, Thyme, Centaury and Ozone Oils against some pathogenic microorganisms, Kafkas. J Med Sci 6:18–22
Baratta MT, Dorman HJ, Deans SG, Figueiredo AC, Barroso JG, Ruperto G (1998) Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragr J 13:235–244
Bellik Y (2014) Total antioxidant activity and antimicrobial potency of the essential oil and oleoresin of Zingiber officinale Roscoe. Asian Pac J Trop Dis 14:40–44
Biljana DV, Tatjana D, Danijela S, Jovanka D (2011) Antimicrobial effect of essential oil isolated from eucalyptus globulus Labill, from Montenegro. Czech J Food Sci 29:277–284
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253
Chavan MJ, Shinde DB, Nirmal SA (2006) Major volatile constituents of Annona squamosa L., bark. Nat Prod Res 20:54–757
Dinagaran S, Sridhar S, Eganathan P (2016) Chemical composition and antioxidant activities of black seed oil (NIGELLA SATIVA L.). IJPSR 7:4473–4479
Gerige SJ, Yadav MKG, Rao M, Ramanjaneyulu (2009) GC–MS analysis of Nigella sativa seeds and antimicrobial activity of its volatile oil. Braz Arch Biol Technol 52:1189–1192
Ishiguro K, Ando T, Maeda O, Ohmiya N, Niwa Y, Kadomatsu K, Goto H (2007) Ginger ingredients reduce viability of gastric cancer cells via distinct mechanisms. Biochem Biophys Res Commun 362:218–223
Johnson OO, Ayoola GA, Adenipekun T (2013) Antimicrobial activity and the chemical composition of the volatile oil blend from Allium sativum (Garlic Clove) and Citrus reticulata (Tangerine Fruit). Int J Pharm Sci Drug Res 5:187–193
Kamaliroostaa ZK, Kamaliroosta L, Elhamirad AH (2013) Isolation and identification of ginger essential oil. J Food Biosci Technol 3:73–80
Kamran JN, Ansari SH, Ali M, Najmi AK (2014) Volatile oil composition of Rosa damascene Mill (Rosaceae). J Pharmacogn Phytochem 2:177–181
Kiran RO, Babu GD (2002) Essential oil composition of Damask rose (Rosa damascena Mill,) distilled under different pressures and temperatures. Flavour Fragr J 17:136–140
Lalit KDC, Bhushan AJ, Sheeba S, Hemant S, Mounesh CDK, Pooja AK (2012) Antimicrobial activity of commercially available essential oils against Streptpcoccus mutans. J Contemp Dent Pract 1(13):71–74
Magdalena ON, Refugio RB, Evelia AF, Alberto GL, Adriana MT, Luz VM (2011) Chemical composition and antimicrobial activity of oregano (Lippia palmeri S. Wats) essential oil. Rev Fitotec Mex 34:11–17
Mahboubi M (2016) Rosa damascena as holy ancient herb with novel applications. J Tradit Complement Med 6:10–16
Maria F, Athanasios K, Ioanna M, Stavros P, Irene T, Virginia P, Ioannis K, Maria P, Elisavet S, Eugenia EB, Athanasios A (2015) Antimicrobial activity of essential oils of cultivated oregano (Origanum vulgare), sage (Salvia officinalis), and thyme (Thymus vulgaris) against clinical isolates of Escherichia coli, Klebsiella oxytoca, and Klebsiella pneumonia. Microb Ecol Health Dis 26:2328
Martınez-Martınez JCYL (2009) Antimicrobial mechanisms of action. Enferm Infecc Microbiol Clin 27:44–52
Milka MM, Vesselin KK, Dimo SK, Ana MD, Angel SG (2014) Chemical composition, in vitro antiradical and antimicrobial activities of Bulgarian Rosa alba L., essential oil against some oral pathogens. Int J Curr Microbiol Appl Sci 3:11–20
Milovanovic MIILJ, Misan AC, Sakac MB, Cabarkapa IS, Saric BM, Matic JJ, Jovanov PT (2009) Evaluation of a GC–MS method for the analysis of oregano essential oil composition. Food Process Qual Saf 3:75–79
Mohamed S, Eman H (2012) Comparative antimicrobial activity of some active constituents of N. sativa L. World Appl Sci J 20:182–189
Morsi NM (2000) Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotic resistant bacteria. Acta Microbiol Pol 49:63–74
Mozaffari FS, Ghorbanli M, Babai A, Farzami SM (2000) The effect of water stress on the seed oil of Nigella sativa, L. J Essent Oil Res 12:36–38
Nickavar B, Mojab F, Javidnia K, Amoli MAR (2003) Chemical Composition of the Fixed and Volatile Oils of Nigella sativa L. from Iran. Z. Naturforsch 58c:629–631
Oyedemi SO, Okoh AI, Mabinya LV, Pirochenva G, Afolayan AJ (2009) The proposed mechanism of bactericidal action of eugenol, γ-terpineol and γ-terpinene against Listeria monocytogenes, Streptococcus pyogenes, Proteus vulgaris and Escherichia coli. Afr J Biotechnol 8:1280–1286
Raid AA, Yazeed AS, Ayesha M, Rabbani SK, Janardhan C, Gupta VC (2014) Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains. Saudi J Biol Sci 21:147–151
Randhawa MA, Al-Ghamdi MS (2002) A review of the pharmaco-therapeutic effects of Nigella sativa. Pak J Med Res 41:77–83
Raquel B, Cristina N, Rafael G (2012) Evaluation of bacterial resistance to essential oils and antibiotics after exposure to oregano and cinnamon essential oils. Food Borne Pathog Dis 9:699–705
Ravi KU, Pratibha D, Shoeb A (2010) Screening of antibacterial activity of six plant essential oils against pathogenic bacterial strains. Asian J Med Sci 2:152–158
Reeves DS (1989) Antibiotic assays. In: Hawkey PM, Lewis DA (eds) Medical bacteriology, a practical approach. IRL Press, Oxford, pp 195–221
Salman MT, Khan RA, Shukla I (2008) Antimicrobial activity of Nigella sativa Linn. Seed oil against multi drug resistant bacteria from clinical isolates. Nat Prod Radiance 7:10–14
Seenivasan P, Manickkam J, Savarimuthu I (2006) In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med 6:1–8
Silva P, Miyata M, Sato DN, Santos AC, Mendes NH, Leite CQ (2010) Rhodococcus equi isolation from sputum of patients with suspected tuberculosis. Mem Inst Oswaldo Cruz 105:199–202
Sonboli A, Salehi P, Yousefzadi M (2004) Anti-microbial activity and chemical composition of the essential oil of Nepeta crispa Willd, from Iran. Z Naturforsch C 59:653–656
Sultan M, Bhatti HN, Iqbal Z (2005) Chemical analysis of essential oil of ginger (Zingiber officinale). Pak J Biol Sci 8:1576–1578
Yuva B (2014) Total antioxidant activity and antimicrobial potency of the essential oil and oleoresin of Zingiber officinale Roscoe. Asian Pac J Trop Dis 4:40–44
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We are grateful to the Department of Food technology, Hamdard University, New Delhi and Department of Clinical Nutrition, College of Applied Medical Sciences, Hail University for providing facilities to carrying out the present study.
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Ashraf, S.A., Al-Shammari, E., Hussain, T. et al. In-vitro antimicrobial activity and identification of bioactive components using GC–MS of commercially available essential oils in Saudi Arabia. J Food Sci Technol 54, 3948–3958 (2017). https://doi.org/10.1007/s13197-017-2859-2
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DOI: https://doi.org/10.1007/s13197-017-2859-2