Abstract
Firmness at harvest determines the postharvest storability and quality of apples. The climate change has altered the physiological processes of fruits and the reliability of ripening indicators typically used to determine the harvest time, compromising fruit firmness. In this study, ‘Golden Delicious’ apples were harvested at several developmental/ripening stages (107, 122, 137, 152 and 167 days after full bloom, DAFB) and evaluated for physicochemical attributes, which were correlated with fruit firmness. The 167 DAFB fruit corresponded to fruit at the commercial harvest. Fruit harvested at 107 and 122 DAFB did not develop the characteristics of ripe fruit while fruit harvested after 137 DAFB ripened normally. Fruit at commercial harvest showed low firmness. The changes of fruit weight, diameter, height, tristimulus color (L* values) as well as the content of total soluble solids and moisture in fruit correlated well with fruit firmness (r values from −0.76 to −0.97). The changes of pectin content, degree of esterification, molecular weight and content of glucose and galactose in pectin showed a positive relationship with firmness (r = 0.62–0.94). The content of protein, galacturonic acid and mineral elements in pectin correlated negatively with firmness (r −0.66 to −0.99). The results demonstrated that commercial harvest was delayed 30 days, compromising fruit firmness. Some underestimated ripening indicators may help in determining the harvest time of apples.
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References
Atkinson CJ, Taylor L, Taylor JM, Lucas AS (1998) Temperature and irrigation effects on the cropping, development and quality of `Cox’s Orange Pippin’ and `Queen Cox’ apples. Sci Hortic 75:59–81. doi:10.1016/S0304-4238(98)00117-4
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem 72:248–254. doi:10.1016/0003-2697(76)90527-3
Chardonnet CO, Charron CS, Sams CE, Conway WS (2003) Chemical changes in the cortical tissue and cell walls of calcium-infiltrated ‘Golden Delicious’ apples during storage. Postharvest Biol Technol 28:97–111. doi:10.1016/S0925-5214(02)00139-4
de Castro E, Biasi WV, Mitcham EJ (2007) Quality of Pink Lady apples in relation to maturity at harvest, prestorage treatments, and controlled atmosphere during storage. HortScience 42:605–610
De Jager A, De Putter H (1999) Preharvest factors and postharvest quality decline of apples. In: International symposium effect of pre- & postharvest factors in fruit storage, pp 103–110. doi: 10.17660/ActaHortic.1999.485.13
De Salvador FR, Fisichella M, Fontanari M (2006) Correlations between fruit size and fruit quality in apple trees with high and standard crop load levels. J Fruit Ornam Plant Res 14:113–122
Felicetti E, Mattheis JP (2010) Quantification and histochemical localization of ascorbic acid in ‘Delicious’, ‘Golden Delicious’, and ‘Fuji’ apple fruit during on-tree development and cold storage. Postharvest Biol Technol 56:56–63. doi:10.1016/j.postharvbio.2009.12.002
Fischer M, Arrigoni E, Amadò R (1994) Changes in the pectic substances of apples during development and postharvest ripening. Part 2: analysis of the pectic fractions. Carbohydr Polym 25:167–175. doi:10.1016/0144-8617(94)90201-1
Gross KC, Sams CE (1984) Changes in cell wall neutral sugar composition during fruit ripening: a species survey. Phytochemistry 23:2457–2461. doi:10.1016/S0031-9422(00)84075-3
Gwanpua SG, Verlinden BE, Hertog MLATM, Nicolai BM, Hendrickx M, Geeraerd A (2016) Slow softening of Kanzi apples (Malus × domestica L.) is associated with preservation of pectin integrity in middle lamella. Food Chem 211:883–891. doi:10.1016/j.foodchem.2016.05.138
Harker FR, Redgwell RJ, Hallett IC, Murray SH, Carter G (2010) Texture of fresh fruit. In: Janick J (ed) Horticultural reviews. Wiley, pp 121–224. doi:10.1002/9780470650646.ch2
Hoehn E, Gasser F, Guggenbühl B, Künsch U (2003) Efficacy of instrumental measurements for determination of minimum requirements of firmness, soluble solids, and acidity of several apple varieties in comparison to consumer expectations. Postharvest Biol Technol 27:27–37. doi:10.1016/S0925-5214(02)00190-4
Hu H, Brown PH (1994) Localization of boron in cell walls of squash and tobacco and its association with pectin (evidence for a structural role of boron in the cell wall). Plant Physiol 105:681–689. doi:10.1104/pp.105.2.681
Hu H, Brown PH, Labavitch JM (1996) Species variability in boron requirement is correlated with cell wall pectin. J Exp Bot 47:227–232. doi:10.1093/jxb/47.2.227
Jin CH, Kashiwagi T, Mizuno M, Terai H, Tsuchida H (1999) Structural changes in the cell-wall pectic polysaccharides accompanied by softening of apple (Malus pumila Mill.) Cultivars Fuji and Kinsei. Food Preserv Sci 25:293–300. doi:10.5891/jafps.25.293
Johnson DS (1992) The effect of flower and fruit thinning on the firmness of ‘Cox’s Orange Pippin’ apples at harvest and after storage. J Hortic Sci Biotechnol 67:95–101. doi:10.1080/00221589.1992.11516225
Johnston JW, Hewett EW, Hertog MLATM (2002) Postharvest softening of apple (Malus domestica) fruit: a review. N Z J Crop Hortic Sci 30:145–160. doi:10.1080/01140671.2002.9514210
Lara I, García P, Vendrell M (2004) Modifications in cell wall composition after cold storage of calcium-treated strawberry (Fragaria × ananassa Duch.) fruit. Postharvest Biol Technol 34:331–339. doi:10.1016/j.postharvbio.2004.05.018
Ortiz A, Graell J, Lara I (2011) Preharvest calcium applications inhibit some cell wall-modifying enzyme activities and delay cell wall disassembly at commercial harvest of ‘Fuji Kiku-8′ apples. Postharvest Biol Technol 62:161–167. doi:10.1016/j.postharvbio.2011.04.014
Peña MJ, Cárpita NC (2004) Loss of highly branched arabinans and debranching of rhamnogalacturonan I accompany loss of firm texture and cell separation during prolonged storage of apple. Plant Physiol 135:1305–1313. doi:10.1104/pp.104.043679
Ramírez-Legarreta MR, Ruiz-Corral JA, Medina-García G, Jacobo-Cuéllar JL, Parra-Quezada RÁ, Ávila-Marioni MR, Amado-Álvarez JP (2011) Perspectivas del sistema de producción de manzano en Chihuahua, ante el cambio climático. Rev Mex Cienc Agric 2:265–279
Ramos-Aguilar OP et al (2015) Effect of ripening and heat processing on the physicochemical and rheological properties of pepper pectins. Carbohydr Polym 115:112–121. doi:10.1016/j.carbpol.2014.08.062
Rascón-Chu A, Martínez-López AL, Carvajal-Millán E, Ponce de León-Renova NE, Márquez-Escalante JA, Romo-Chacón A (2009) Pectin from low quality ‘Golden Delicious’ apples: composition and gelling capability. Food Chem 116:101–103. doi:10.1016/j.foodchem.2009.02.016
Redgwell RJ, Fischer M, Kendal E, MacRae EA (1997) Galactose loss and fruit ripening: high-molecular-weight arabinogalactans in the pectic polysaccharides of fruit cell walls. Planta 203:174–181. doi:10.1007/s004250050179
Saei A, Tustin DS, Zamani Z, Talaie A, Hall AJ (2011) Cropping effects on the loss of apple fruit firmness during storage: The relationship between texture retention and fruit dry matter concentration. Sci Hortic 130:256–265. doi:10.1016/j.scienta.2011.07.008
Saftner RA (1999) The potential of fruit coating and film treatments for improving the storage and shelf-life qualities of `Gala’ and `Golden Delicious’ Apples. J Am Soc Hortic Sci 124:682–689
Salas NA, Molina-Corral FJ, González-Aguilar GA, Otero A, Sepulveda DR, Olivas GI (2011) Volatile production by ‘Golden Delicious’ apples is affected by preharvest application of aminoethoxyvinylglycine. Sci Hortic 130:436–444. doi:10.1016/j.scienta.2011.07.017
Siddiqui S, Brackmann A, Streif J, Bangerth F (1996) Controlled atmosphere storage of apples: cell wall composition and fruit softening. J Hortic Sci Biotechnol 71:613–620. doi:10.1080/14620316.1996.11515441
Stow J (1993) Effect of calcium ions on apple fruit softening during storage and ripening. Postharvest Biol Technol 3:1–9. doi:10.1016/0925-5214(93)90021-T
Volz RK, Harker FR, Hallet IC, Lang A Development of texture in apple fruit-a biophysical perspective. In, 2004. International Society for Horticultural Science (ISHS), Leuven, Belgium, pp 473-479. doi:10.17660/ActaHortic.2004.636.58
Voragen AGJ, Schols HA, Pilnik W (1986) Determination of the degree of methylation and acetylation of pectins by h.p.l.c. Food Hydrocoll 1:65–70. doi:10.1016/S0268-005X(86)80008-X
Wang K, Hua X, Yang R, Kang J, Zhang W (2014) Hydrodynamic behavior and gelling properties of sunflower head pectin in the presence of sodium salts. Food Hydrocoll 36:238–244. doi:10.1016/j.foodhyd.2013.09.011
Yoshioka H, Aoba K, Kashimura Y (1992) Molecular weight and degree of methoxylation in cell wall polyuronide during softening in pear and apple fruit. J Am Soc Hortic Sci 117:600–606
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This research was funded by the Fondo Mixto CONACYT- Gobierno del Estado de Chihuahua (Project Clave: CHIH-2012-C03-194579). The authors thank Emilio Ochoa for his technical assistance.
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Ornelas-Paz, J.d., Quintana-Gallegos, B.M., Escalante-Minakata, P. et al. Relationship between the firmness of Golden Delicious apples and the physicochemical characteristics of the fruits and their pectin during development and ripening. J Food Sci Technol 55, 33–41 (2018). https://doi.org/10.1007/s13197-017-2758-6
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DOI: https://doi.org/10.1007/s13197-017-2758-6