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Determination of anthocyanins and non-anthocyanin polyphenols by ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI–MS) in jussara (Euterpe edulis) extracts

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Abstract

This work aimed to propose two analytical methods for the quantitative and qualitative analysis of major anthocyanins and non-anthocyanin phenolic compounds in jussara (Euterpe edulis) extracts, using ultra performance liquid chromatography-mass spectrometry. These methods were evaluated for selectivity, precision, linearity, detection and quantification limits. The complete separation of 5 anthocyanins and 22 non-anthocyanins polyphenols was achieved in 4.5 and 7 min, respectively. Limits of detection ranged from 0.55 to 9.24 µg/L, with relative standard deviation for concentration up to 7.0%. In jussara extract, 13 of the 27 analytes were characterized. The dominant compound was cyanidin-3-O-rutinoside, representing about 73% of the total phenolic compounds content (approximately 23 mg/g of extract in dry weight). Other phenolic compounds found in the extract were: cyanidin-3-O-glucoside, pelargonidin-3-O-glucoside, quercetin, rutin, myricetin, kaempferol, kaempferol-3-O-rutinoside, luteolin, apigenin, catechin, ellagic acid and 4,5-dicaffeoylquinic acid.

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Acknowledgements

The authors thank FAPESP (2009/54137-1), Capes and CNPq for their financial support. Glaucia Santos Vieira thanks CNPq for Ph.D. assistantships 140579/2010-9. The authors also are very grateful to Jonas B. Alonso by statistical support, Mauricio A. Rostagno and Noelia Manchón for the loan of some standards used in this project.

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Authors and Affiliations

  1. Department of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), 80, Monteiro Lobato Street, P.O. Box 6121, Campinas, SP, 13083-862, Brazil

    Gláucia S. Vieira, Mariana T. C. Machado, Vanessa M. Silva & Miriam D. Hubinger

  2. Waters Corporation Brazil, 125, Alphaville, São Paulo, SP, 06455-020, Brazil

    Anna S. F. Marques

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  1. Gláucia S. Vieira
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  2. Anna S. F. Marques
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  3. Mariana T. C. Machado
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  4. Vanessa M. Silva
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  5. Miriam D. Hubinger
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Correspondence to Gláucia S. Vieira.

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Vieira, G.S., Marques, A.S.F., Machado, M.T.C. et al. Determination of anthocyanins and non-anthocyanin polyphenols by ultra performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI–MS) in jussara (Euterpe edulis) extracts. J Food Sci Technol 54, 2135–2144 (2017). https://doi.org/10.1007/s13197-017-2653-1

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  • DOI: https://doi.org/10.1007/s13197-017-2653-1

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