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Chemical composition and bioactivity of essential oils from flower and fruit of Thymbra capitata and Thymus species

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Abstract

Thymbra capitata and Thymus species are commonly known as thyme in Spain and they are currently used as culinary herbs, as well as for ornamental, aromatizing and traditional medicinal purposes. Given the economic importance of thyme oils, many thyme species have been studied, and their essential oils (EOs) and other volatile-containing extracts have been chemically characterized. The aim of this study was to evaluate the antioxidant, antimicrobial and cytotoxic activities as well as the phytochemical composition of the EOs from fruits and flowers of T. capitata and Thymus species. High antioxidant capacity has been observed and related to the relative amounts of terpenoid and phenolic with good antioxidant properties. The antioxidant activities decreased as follows: TAC > TZ > TC > TM. The results of antimicrobial activity showed that all tested EOs were active against all tested microbial species, including Gram-positive and negative bacteria. The minimum inhibition concentration values obtained ranged from 0.1 to 0.01 μL/mL. Furthermore, all EOs assayed induced cell death in both human epitheloid cervix carcinoma and histiocytic leukemia cell lines. Overall, the results of this study indicates that the EOs from fruits and flowers of T. capitata and Thymus species posses interesting antioxidant properties and represent a potential source of medicine for the treatment of infectious diseases and cancer.

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References

  • Abbaszadeh S, Sharifzadeh A, Shokri H et al (2014) Antifungal efficacy of thymol, carvacrol, eugenol and menthol as alternative agents to control the growth of food-relevant fungi. J Mycol Médicale/J Med Mycol 24:e51–e56. doi:10.1016/j.mycmed.2014.01.063

    Article  CAS  Google Scholar 

  • Amensour M, Sendra E, Abrini J et al (2009) Total phenolic content and antioxidant activity of myrtle (Myrtus communis) extracts. Nat Prod Commun 4:819–824

    CAS  Google Scholar 

  • Amorati R, Foti MC, Valgimigli L (2013) Antioxidant activity of essential oils. J Agric Food Chem 61:10835–10847. doi:10.1021/jf403496k

    Article  CAS  Google Scholar 

  • Bruneton J (2001) Aceites esenciales. Factores de variabilidad de los aceites esenciales. In: Bruneton J (ed) Farmacognosia. Acribia S.A., Zaragoza, pp. 488–491

  • Cano A, Acosta M, Arnao MB (2000) A method to measure antioxidant activity in organic media: application to lipophilic vitamins. Redox Rep 5:365–370. doi:10.1179/135100000101535933

    Article  CAS  Google Scholar 

  • Council of Europe (1997) European pharmacopoeia, 3rd edn. Council of Europe, Strasbourg

    Google Scholar 

  • de Sousa JP, de Oliveira KÁR, de Figueiredo RCBQ, de Souza EL (2015) Influence of carvacrol and 1,8-cineole on cell viability, membrane integrity, and morphology of aeromonas hydrophila cultivated in a vegetable-based broth. J Food Prot 78:424–429. doi:10.4315/0362-028X.JFP-14-242

    Article  Google Scholar 

  • Deb DD, Parimala G, Saravana Devi S, Chakraborty T (2011) Effect of thymol on peripheral blood mononuclear cell PBMC and acute promyelotic cancer cell line HL-60. Chem Biol Interact 193:97–106. doi:10.1016/j.cbi.2011.05.009

    Article  Google Scholar 

  • Delgado Adámez J, Gamero Samino E, Valdés Sánchez E, González-Gómez D (2012) In vitro estimation of the antibacterial activity and antioxidant capacity of aqueous extracts from grape-seeds (Vitis vinifera L.). Food Control 24:136–141. doi:10.1016/j.foodcont.2011.09.016

    Article  Google Scholar 

  • Döll-Boscardin PM, Sartoratto A, Sales Maia BH et al (2012) In vitro cytotoxic potential of essential oils of Eucalyptus benthamii and its related terpenes on tumor cell lines. Evid Based Complement Alternat Med 2012:1–8. doi:10.1155/2012/342652

    Article  Google Scholar 

  • Dorman HJD, Surai P, Deans SG (2000) In vitro antioxidant activity of a number of plant essential oils and phytoconstituents. J Essent Oil Res 12:241–248

    CAS  Google Scholar 

  • Ehrnhöfer-Ressler MM, Fricke K, Pignitter M et al (2013) Identification of 1,8-cineole, borneol, camphor, and thujone as anti-inflammatory compounds in a Salvia officinalis L. infusion using human gingival fibroblasts. J Agric Food Chem 61:3451–3459. doi:10.1021/jf305472t

    Article  Google Scholar 

  • Figueiredo AC, Barroso JG, Pedro LG (2010) Volatiles from Thymbra and Thymus species of the western mediterranean basin, portugal and macaronesia. Nat Prod Commun 5:1465–1476

    CAS  Google Scholar 

  • Gilles M, Zhao J, An M, Agboola S (2010) Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chem 119:731–737. doi:10.1016/j.foodchem.2009.07.021

    Article  CAS  Google Scholar 

  • Gonçalves MJ, Cruz MT, Cavaleiro C et al (2010) Chemical, antifungal and cytotoxic evaluation of the essential oil of Thymus zygis subsp. sylvestris. Ind Crops Prod 32:70–75. doi:10.1016/j.indcrop.2010.03.005

    Article  Google Scholar 

  • Heim KE, Tagliaferro AR, Bobilya DJ (2002) Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem 13:572–584

    Article  CAS  Google Scholar 

  • Hosseini Behbahani M, Ghasemi Y, Khoshnoud MJ et al (2013) Volatile oil composition and antimicrobial activity of two Thymus species. Pharmacogn J 5:77–79. doi:10.1016/j.phcgj.2012.09.002

    Article  Google Scholar 

  • Jaberian H, Piri K, Nazari J (2013) Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chem 136:237–244. doi:10.1016/j.foodchem.2012.07.084

    Article  CAS  Google Scholar 

  • Jayakumar S, Madankumar A, Asokkumar S et al (2012) Potential preventive effect of carvacrol against diethylnitrosamine-induced hepatocellular carcinoma in rats. Mol Cell Biochem 360:51–60. doi:10.1007/s11010-011-1043-7

    Article  CAS  Google Scholar 

  • Klein G, Rüben C, Upmann M (2013) Antimicrobial activity of essential oil components against potential food spoilage microorganisms. Curr Microbiol 67:200–208. doi:10.1007/s00284-013-0354-1

    Article  CAS  Google Scholar 

  • Kumar D, Rawat DS (2013) Synthesis and antioxidant activity of thymol and carvacrol based Schiff bases. Bioorg Med Chem Lett 23:641–645. doi:10.1016/j.bmcl.2012.12.001

    Article  Google Scholar 

  • Kumar P, Mishra S, Malik A, Satya S (2013) Housefly (Musca domestica L.) control potential of Cymbopogon citratus Stapf. (Poales: Poaceae) essential oil and monoterpenes (citral and 1,8-cineole). Parasitol Res 112:69–76. doi:10.1007/s00436-012-3105-5

    Article  Google Scholar 

  • Limem-Ben Amor I, Neffati A, Ben Sgaier M et al (2008) Antimicrobial activity of essential oils isolated from Phlomis crinita Cav. ssp. mauritanica Munby. J Am Oil Chem Soc 85:845–849. doi:10.1007/s11746-008-1272-4

    Article  CAS  Google Scholar 

  • Marchese A, Orhan IE, Daglia M et al (2016) Antibacterial and antifungal activities of thymol: a brief review of the literature. Food Chem 210:402–414. doi:10.1016/j.foodchem.2016.04.111

    Article  CAS  Google Scholar 

  • Mateos S, Valls J, Nadal M, Arola L (2001) Estudio de madurez fenólica sobre diferentes variedades tintas y su relación con el color de los vinos. Tecnología del Vino 2:45–50

    Google Scholar 

  • Miguel MG (2010a) Antioxidant activity of medicinal and aromatic plants. A review. Flavour Fragr J 25:291–312. doi:10.1002/ffj.1961

    Article  CAS  Google Scholar 

  • Miguel MG (2010b) Antioxidant and anti-inflammatory activities of essential oils: a short review. Molecules 15:9252–9287. doi:10.3390/molecules15129252

    Article  Google Scholar 

  • Miguel G, Guerrero C, Rodrigues H et al (1999) Study of the substrate and fertilization effects on the production of essentials oils by Thymus mastichina (L.) L. ssp. mastichina cultivated in pots. In: Anaç D, Martin-Prével P (eds) Improved crop quality by nutrient management. Springer, Dordrecht, pp 201–204

    Chapter  Google Scholar 

  • Miguel G, Simões M, Figueiredo AC et al (2004) Composition and antioxidant activities of the essential oils of Thymus caespititius, Thymus camphoratus and Thymus mastichina. Food Chem 86:183–188. doi:10.1016/j.foodchem.2003.08.031

    Article  CAS  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  Google Scholar 

  • Nazzaro F, Fratianni F, De Martino L et al (2013) Effect of essential oils on pathogenic bacteria. Pharmaceuticals 6:1451–1474. doi:10.3390/ph6121451

    Article  Google Scholar 

  • Pinelo M, Rubilar M, Jerez M et al (2005) Effect of solvent, temperature, and solvent-to-solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. J Agric Food Chem 53:2111–2117. doi:10.1021/jf0488110

    Article  CAS  Google Scholar 

  • Quiroga PR, Asensio CM, Nepote V (2015) Antioxidant effects of the monoterpenes carvacrol, thymol and sabinene hydrate on chemical and sensory stability of roasted sunflower seeds. J Sci Food Agric 95:471–479. doi:10.1002/jsfa.6744

    Article  CAS  Google Scholar 

  • Ruberto G, Baratta MT (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem 69:167–174. doi:10.1016/S0308-8146(99)00247-2

    Article  CAS  Google Scholar 

  • Vilela GR, de Almeida GS, D’Arce MABR et al (2009) Activity of essential oil and its major compound, 1,8-cineole, from Eucalyptus globulus Labill., against the storage fungi Aspergillus flavus Link and Aspergillus parasiticus Speare. J Stored Prod Res 45:108–111. doi:10.1016/j.jspr.2008.10.006

    Article  CAS  Google Scholar 

  • Viuda-Martos M, Mohamady MA, Fernández-López J et al (2011) In vitro antioxidant and antibacterial activities of essentials oils obtained from Egyptian aromatic plants. Food Control 22:1715–1722. doi:10.1016/j.foodcont.2011.04.003

    Article  CAS  Google Scholar 

Download references

Acknowledgements

J. Espino and M. Garrido hold a research post-doctoral fellowship from Government of Extremadura (jointly financed by the European Regional Development Fund (ERDF); Refs. PO14011 and PO14013, respectively).

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Authors and Affiliations

  1. Technological Institute of Food and Agriculture (INTAEX), Gobierno de Extremadura, Av. Adolfo Suárez s/n, 06071, Badajoz, Spain

    Jonathan Delgado-Adámez, Maria Elena Bote, María C. Fuentes-Pérez & Daniel Martín-Vertedor

  2. Department of Physiology, Neuroimmunophysiology and Chrononutrition Research Group, Faculty of Science, University of Extremadura, Av. Elvas s/n, 06006, Badajoz, Spain

    María Garrido & Javier Espino

Authors
  1. Jonathan Delgado-Adámez
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  2. María Garrido
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  3. Maria Elena Bote
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  4. María C. Fuentes-Pérez
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  5. Javier Espino
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  6. Daniel Martín-Vertedor
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Correspondence to Jonathan Delgado-Adámez.

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Delgado-Adámez, J., Garrido, M., Bote, M.E. et al. Chemical composition and bioactivity of essential oils from flower and fruit of Thymbra capitata and Thymus species. J Food Sci Technol 54, 1857–1865 (2017). https://doi.org/10.1007/s13197-017-2617-5

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  • DOI: https://doi.org/10.1007/s13197-017-2617-5

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