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Application of response surface methodology for the optimization of supercritical fluid extraction of essential oil from pomegranate (Punica granatum L.) peel

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Abstract

Essential oils and volatile components of pomegranate (Punica granatum L.) peel of the Malas variety from Meybod, Iran, were extracted using supercritical fluid extraction (SFE) and hydro-distillation methods. The experimental parameters of SFE that is pressure, temperature, extraction time, and modifier (methanol) volume were optimized using a central composite design after a (24–1) fractional factorial design. Detailed chemical composition of the essential oils and volatile components obtained by hydro-distillation and optimum condition of the supercritical CO2 extraction were analyzed by GC–MS, and seventy-three and forty-six compounds were identified according to their retention indices and mass spectra, respectively. The optimum SFE conditions were 350 atm pressure, 55 °C temperature, 30 min extraction time, and 150 µL methanol. Results showed that oleic acid, palmitic acid and (−)-Borneol were major compounds in both extracts. The optimum extraction yield was 1.18 % (w/w) for SFE and 0.21 % (v/w) for hydro-distillation.

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  1. Department of Applied Chemistry, Faculty of Chemistry, Shahid Beheshti University, G. C., Tehran, Iran

    Katayoun Mahdavi Ara & Farhad Raofie

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  1. Katayoun Mahdavi Ara
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  2. Farhad Raofie
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Correspondence to Farhad Raofie.

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Ara, K.M., Raofie, F. Application of response surface methodology for the optimization of supercritical fluid extraction of essential oil from pomegranate (Punica granatum L.) peel. J Food Sci Technol 53, 3113–3121 (2016). https://doi.org/10.1007/s13197-016-2284-y

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  • DOI: https://doi.org/10.1007/s13197-016-2284-y

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