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Partial extraction and identification of phenolics in Amla (Emblica officinalis) seed coat powder

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Abstract

RSM was used to determine the optimum conditions for maximum recovery of phenolics from amla seed coat powder. At optimum conditions, TPC (total phenolic content) content and % DPPH free radical scavenging activity of extract were found to be 7.02 mg GAE / mL of extract and 42.69 %, respectively. Phenolics were extracted in water, diethyl ether, butanol and ethyl acetate. % DPPH free radical scavenging (61.07 %) activity was maximum in ethyl acetate fraction. The major phenolics in ethyl acetate extract were gallic, catechin, caffeic, quercetin, p-coumaric, vanillic and chlorogenic acids. Ethyl acetate extract was further fractionated with six fractions using methanol: water by column chromatography. Fraction V showed highest DPPH scavenging activity. The phenolics in different fractions of ethyl acetate extract were also estimated by GC-MS after derivatization. Caeffic, p-coumaric, hydroxy caeffic, vanillic, gallic and p-hydroxybenzoic acid were identified. Other components like tetracontane-1,40-diol and octadecanoic acid could also be identified.

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Acknowledgments

Authors are thankful for the financial support received from University Grant Commission (UGC), India.

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Authors and Affiliations

  1. Department of Food Engineering & Technology, Tezpur University, Napaam, Assam, 784028, India

    Poonam Mishra & Charu Lata Mahanta

  2. Department of Chemical Science, Tezpur University, Napam, Assam, 784028, India

    Nipu Dutta

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  1. Poonam Mishra
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  2. Nipu Dutta
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  3. Charu Lata Mahanta
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Correspondence to Poonam Mishra.

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Mishra, P., Dutta, N. & Mahanta, C.L. Partial extraction and identification of phenolics in Amla (Emblica officinalis) seed coat powder. J Food Sci Technol 52, 6990–7001 (2015). https://doi.org/10.1007/s13197-015-1835-y

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  • DOI: https://doi.org/10.1007/s13197-015-1835-y

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