Abstract
The antioxidative capacity of six different tissue hydrolysates (porcine colon, heart and neck and bovine lung, kidney and pancreas) were tested by three different assays monitoring iron chelation, ABTS radical scavenging and inhibition of lipid oxidation in emulsions, respectively. The hydrolysates were also investigated with respect to amino acid composition and peptide size distribution. The hydrolysates contained peptides ranging from 20 kDa to below 100 Da with a predominance of peptides with low molecular weight (53.8 to 89.0 % below 3 kDa). All hydrolysates exhibited antioxidant activity as assessed with all three methods; inhibition of lipid oxidation ranging from 72 to 88 % (at a final protein concentration of 7 mg/mL), iron chelation capacity from 23 to 63 % (at 1.1 mg/mL), and ABTS radical scavenging from 38 to 50 % (at 10 μg /mL). The antioxidant activity did not correlate with the proportion of low molecular weight peptides in the hydrolysed tissues, but with the content of specific amino acid residues. The ABTS radical scavenging capacity of the tissues was found to correlate with the content of Trp, Tyr, Met and Arg, whereas the ability to inhibit the oxidation of lineoleic acid correlated with the content of Glu and His. The chosen animal by-products thus represent a natural source of antioxidants with potential for food application.
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Acknowledgments
Thanks to Erik T. Hansen and Kim Dam Nielsen from Dat-Schaub for producing the hydrolysates and for providing support and help. Thanks to Lene Meinert from the Danish Meat Research Institute for data sharing, support and help, and thanks to Daniel Tsegay Berhe from Food Science, University of Copenhagen, for help with the PCA analysis. This work was financed by inSPIRe and the Pig Levy Fund.
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Damgaard, T., Lametsch, R. & Otte, J. Antioxidant capacity of hydrolyzed animal by-products and relation to amino acid composition and peptide size distribution. J Food Sci Technol 52, 6511–6519 (2015). https://doi.org/10.1007/s13197-015-1745-z
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DOI: https://doi.org/10.1007/s13197-015-1745-z