Abstract
Hepcidins are small cysteine-rich antimicrobial peptides that play a vital role in immunity against pathogen invasion. Here, a hepcidin (Cshep) from Centropristis striata was described, which is considered as a valuable aquaculture marine species in China. The open reading frame consisted of 273 bp. Eight conserved cysteine residues were identified. Phylogenetic analysis showed that Cshep had a relatively close relationship with the hepcidin from Epinephelus moara. Quantitative real-time PCR analysis demonstrated that Cshep was highly expressed in liver and significantly up-regulated when challenged with Vibrio harveyi. In addition, the synthetic Cshep peptide had a high antimicrobial activity against V. harveyi, but low against other pathogenic bacteria tested in this study. The killing kinetics analysis revealed that Cshep had a fast bactericidal effect on V. harveyi. These results suggested that Cshep may be involved in the immune response of C. striata against V. harveyi infection.
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The Youth Foundation of Guangxi Zhuang Autonomous Region, China under contract No. 2018GXNSFBA050032; the Innovation Driven Development Foundation of Guangxi under contract Nos AD19245135 and AD19245161; the Doctoral Research Startup Fund of the Fourth Institute of Oceanography, Ministry of Natural Resources under contract Nos 201803 and 201806; the Fund of Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, China under contract No. FREU2016-04.
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Ma, X., Qiao, Y., Shao, Y. et al. Antimicrobial peptide hepcidin contributes to host defense of Centropristis striata against Vibrio harveyi challenge. Acta Oceanol. Sin. 40, 61–66 (2021). https://doi.org/10.1007/s13131-021-1776-4
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DOI: https://doi.org/10.1007/s13131-021-1776-4