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Rock Tea extract (Jasonia glutinosa) relaxes rat aortic smooth muscle by inhibition of L-type Ca2+ channels

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Abstract

In traditional herbal medicine, Rock Tea (Jasonia glutinosa) is known for its prophylactic and therapeutic value in various disorders including arterial hypertension. However, the mechanism by which Rock Tea exerts blood pressure-lowering actions has not been elucidated yet. Our aim was to demonstrate vasorelaxing effects of Rock Tea extract and to reveal its possible action mechanism. Isometric myography was conducted on high-K+-precontracted rings from rat thoracic aorta and tested extracts at concentrations of 0.5–5 mg/ml. Whole-cell patch-clamp experiments were performed in rat aortic vascular smooth muscle cells (line A7r5) to determine blocking effects on L-type Ca2+ channels. Rock Tea extract relaxed the aorta contracted by high [K+] concentration dependently with an EC50 of ≈2.4 mg/ml and produced ≈75 % relaxation at the highest concentration tested. The L-type Ca2+ channel blocker, verapamil (10−6 M), had similar effects. Rock Tea extract had no effect in nominally Ca2+-free high-K+ buffer but significantly inhibited contractions to re-addition of Ca2+. Rock Tea extract inhibited the contractions induced by the L-type Ca2+ channel activator Bay K 8644 (10−5 M) and by phenylephrine (10−6 M). Rock Tea extract and Y-27632 (10−6 M), Rho-kinase inhibitor, had similar effects and the respective effects were not additive. Patch-clamp experiments demonstrated that Rock Tea extract (2.5 mg/ml) virtually abolished L-type Ca2+ currents in A7r5. We conclude that Rock Tea extract produced vasorelaxation of rat aorta and that this relaxant effect is mediated by inhibition of L-type Ca2+ channels. Rock Tea extracts may be of phytomedicinal value for prevention and adjuvant treatment of hypertension and other cardiovascular diseases.

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Acknowledgments

This study was supported by the Government of Aragón (GIMACES, Grant no. E-02 and B61) and by the Fondo Social Europeo “Construyendo Europa desde Aragón”. RK and AOV were supported by the Deutsche Forschungsgemeinschaft (KO1899/11-1 to RK) and the Fondo de Investigación Sanitaria (Red HERACLES RD12/0042/0014).

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Authors and Affiliations

  1. Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, Spain

    Marta Sofía Valero, Irene Garrido, Elisa Langa, César Berzosa, Víctor López & Carlota Gómez-Rincón

  2. Aragon Institute of Health Sciences and IIS, Zaragoza, Spain

    Aida Oliván-Viguera & Ralf Köhler

  3. Department of Pharmacology and Physiology, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain

    María Divina Murillo

  4. Fundación Agencia Aragonesa para la Investigación y Desarrollo (ARAID), Zaragoza, Spain

    Ralf Köhler

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  1. Marta Sofía Valero
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  2. Aida Oliván-Viguera
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  3. Irene Garrido
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  4. Elisa Langa
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  8. María Divina Murillo
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  9. Ralf Köhler
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Correspondence to Marta Sofía Valero.

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Valero, M.S., Oliván-Viguera, A., Garrido, I. et al. Rock Tea extract (Jasonia glutinosa) relaxes rat aortic smooth muscle by inhibition of L-type Ca2+ channels. J Physiol Biochem 71, 785–793 (2015). https://doi.org/10.1007/s13105-015-0442-8

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  • DOI: https://doi.org/10.1007/s13105-015-0442-8

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