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Cryptostrobin and catechin isolated from Eugenia mattosii D. Legrand leaves induce endothelium-dependent and independent relaxation in spontaneously hypertensive rat aorta

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Abstract

Background

Considering the therapeutic potential of phenolic compounds, the purpose of the present study was to investigate the mechanisms involved in the relaxation induced by cryptostrobin and catechin, isolated from Eugenia mattosii D. Legrand leaves, in the aorta of spontaneously hypertensive rats (SHR).

Methods

The thoracic aorta was isolated from SHR and kept in the organ bath system by recording contractile or relaxant responses.

Results

The addition of cumulative concentrations of cryptostrobin and catechin induced endothelium-dependent and-independent relaxation in aorta rings from SHR, as well as both compounds were effective in reducing phenylephrine-induced contraction. Pretreatment of aortic rings with Nm-nitro-L-arginine methylester (L-NAME, an inhibitor of nitric oxide synthase) or 1H-[1,2,4] oxadiazolo[4,3-a] quinoxalin-1-one (ODQ, an inhibitor of soluble guanylate cyclase), resulted in a significant change of relaxant effect induced by catechin, and a slight influence on cryptostrobin-induced relaxation. Muscarinic receptor and potassium channels are involved in catechin-induced relaxation as assessed using atropine (a muscarinic receptor antagonist), tetraethylammonium (a non-selective K+ channel blocker) and glibenclamide (an ATP-sensitive K+ channel blocker). Conversely, cryptostrobin, but not catechin, blunted the contraction induced by the addition of phenylephrine in a calcium-free solution. Besides that, cryptostrobin attenuated the contraction of rat aorta rings induced by internal Ca2+ release and external Ca2+ influx.

Conclusions

These findings indicated that cryptostrobin and catechin alter vascular smooth muscle reactivity, and this effect may be involved, at least in part, by enhancing the endothelium NO/cGMP pathway and potassium channels activation. In addition, cryptostrobin reduced the phenylephrine, KC1 and CaCl2-induced contractions in a calcium-free solution.

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Authors and Affiliations

  1. Programa de Pos-Graduacao em Ciencias Farmaceuticas, Universidade do Vale do Itajai (UNIVALI), Itajai, SC, Brazil

    Giovana Vechi, Rita Cássia Melo Vilhena Andrade Fonseca de da Silva, Priscila de Souza, Luísa Mota da Silva, Sérgio Faloni de Andrade & Valdir Cechinel Filho

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Vechi, G., da Silva, R.C.M.V.A.F.d., de Souza, P. et al. Cryptostrobin and catechin isolated from Eugenia mattosii D. Legrand leaves induce endothelium-dependent and independent relaxation in spontaneously hypertensive rat aorta. Pharmacol. Rep 71, 950–957 (2019). https://doi.org/10.1016/j.pharep.2019.05.006

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