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Effect of extraction solvent on in vitro anti-inflammatory, antioxidant activity, total phenol and flavonoid contents in Codonopsis lanceolata

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Abstract

Many plant products from medicinal herbs are historically important for the treatment and prevention of diseases. Medicinal plants are showing a wide range of activities like antioxidant, anti-inflammatory, and anti-aging. This study was investigated to evaluate the phenolic content, DPPH, ABTS radical scavenging rate, and anti-inflammatory activity from Codonopsis lanceolata (CL) root extract. Total polyphenol and flavonoid content showed the highest amount in 30% EtOH extract of CL and followed by 90 °C hot water extract. The DPPH, ABTS radical scavenging activity was also found that the increase was proportional to the concentration, the highest amount in 30% EtOH extract. Cell viability of RAW 264.7 cell for CL extracts was not significantly changed by extracts up to a concentration of 200 µg/mL, and it was confirmed that the macrophage cell showed little toxicity. LPS-treated experimental group significantly inhibited NO production. The effect of extracts of CL against inflammatory mediators (TNF-α, IL-6, and IL-1β) production showed that the anti-inflammatory activity was most excellent in 30% EtOH. These results suggested that CL extracts tested here have potential anti-inflammatory and antioxidant activity, and these effects are differences depending on the extraction solvent.

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References

  • Ajuebor MN, Virag L, Flower RJ, Perretti M, Szabo C (1998) Role of inducible nitric oxide synthase in the regulation of neutrophil migration in zymosan-induced inflammation. Immunology 95:625–630

    Article  CAS  Google Scholar 

  • Alexander C, Rietschel ET (2001) Bacterial lipopolysaccharides and innate immunity. J Endotoxin Res 7:167–202

    CAS  PubMed  Google Scholar 

  • Balkwill F, Charles KA, Mantovani A (2005) Smoldering and polarized inflammation in the initiation and promotion of malignant disease. Cancer Cell 7(3):211–217

    Article  CAS  Google Scholar 

  • Boo HO, Shin JH, Shin JS, Choung ES, Bang MA, Choi KM, Song WS (2012) Assessment on antioxidant potential and enzyme activity of some economic resource plants. Korean J Plant Res 25(3):349–356

    Article  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Food Sci Technol 28:25–30

    CAS  Google Scholar 

  • Choi EY, Heo SI, Kwon YS, Kim MJ (2016) Anti-oxidant activity and anti-inflammatory effects of Spiraea fritschiana Schneid extract. Korean J Med Crop Sci 24(1):31–37

    Article  Google Scholar 

  • College JNM (1977) Dictionary of Chinese materia medica. Shanghai Science and Technology Publisher, Shanghai, pp 195–196

    Google Scholar 

  • Dempsey PW, Vaidya SA, Cheng G (2003) The art of war: innate and adaptive immune response. Cell Mol Life Sci 60:2604–2621

    Article  CAS  Google Scholar 

  • Feldmann M, Brennan FM, Maini RN (1996) Role of cytokines in rheumatoid arthritis. Annu Rev Immunol 14:397–440

    Article  CAS  Google Scholar 

  • Gülcin I, Huyut Z, Elmastas M, Aboul-Enein A (2010) Radical scavenging and antioxidant activity of tannic acid. Arab J Chem 3:43–53

    Article  Google Scholar 

  • Ialenti A, Moncada S, Di Rosa M (1993) Modulation of adjuvant arthritis by endogenous nitric oxide. Br J Pharmacol 110:701–706

    Article  CAS  Google Scholar 

  • Joh EH, Lee IA, Han SJ, Chae SJ, Kim DH (2010) Lancemaside A ameliorates colitis by inhibiting NF-κB activation in TNBS-induced colitis mice. Int J Colorectal Dis 25:545–551

    Article  Google Scholar 

  • Ju JH, Kim JS, Kang SS, Son KH, Chang HW, Kim HP (2010) Anti-inflammatory and anti-arthritic activity of total flavonoids of the roots of Sophora flavescens. J Ethnopharmacol 127(3):589–595

    Article  Google Scholar 

  • Karin M, Ben-Neriah Y (2000) Phosphorylation meets ubiquitination: the control of NF-kB activity. Annu Rev J Immunol 18:621–663

    Article  CAS  Google Scholar 

  • Kim ID, Dhungana SK, Kim HR, Shin DH (2017) Quality characteristics and antioxidant potential of seeds of native Korean persimmon genotypes. Korean J Plant Res 30(6):670–678

    CAS  Google Scholar 

  • Kim MS, Kim N, Kwon SJ, Kim HR, Lee DY, Oh MJ, Kim HJ, Lee CH, Oh CH (2018) Anti-inflammatory and immune regulatory effects of Aucklandia lappa Decne 70% ethanol extract. Korean J Med Crop Sci 26(1):8–18

    Google Scholar 

  • Lee KT, Choi J, Jun WT, Nam JH, Jung HJ, Park HJ (2002) Structure of a new echinocystic acid bisdesmoside isolated from Codonopsis lanceolata roots and the cytotoxic activity of prosapogenins. J Agri Food Chem 50:4190–4193

    Article  CAS  Google Scholar 

  • Leong LP, Shui G (2002) An investigation of antioxidant capacity of fruits in Singapore markets. Food Chem 76:69–75

    Article  CAS  Google Scholar 

  • Liu SC, Lin JT, Wang CK (2009) Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis sonn.) flowers. Food Chem 114:577–581

    Article  CAS  Google Scholar 

  • Marja PK, Anu IH, Heikki JV, Jussi-Pekka R, Kalevi P, Tytti SK, Marina H (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agri Food Chem 47:3954–3962

    Article  Google Scholar 

  • Pokharel YR, Liu QH, Oh JW, Woo R, Kang KW (2007) 4-Hydroxykobusin inhibits the induction of nitric oxide synthase by inhibiting NF-kB and AP-1 activation. Biol Pharm Bull 30:1097–1101

    Article  CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Evans CR (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 26(9–10):1231–1237

    Article  CAS  Google Scholar 

  • Rice-Evans C, Miller NJ, Bolwell GP, Bramley PM, Pridham JB (1995) The relative antioxidants activities of plant-derived polyphenolic flavonoids. Free Radic Res 22:375–383

    Article  CAS  Google Scholar 

  • Rockel P, Strube F, Rockel A, Wildt J, Kaiser WM (2002) Regulation of nitric oxide (NO) production by plant nitrate reductase in vivo and in vitro. J Exp Bot 53(366):103–110

    Article  CAS  Google Scholar 

  • Sanchez-Moreno C, Larrauri JA, Saura-Calixto F (1999) Free radical scavenging capacity and inhibition of lipid oxidation of wines, grape juices and related polyphenolic constituents. Food Res Int 32:407–412

    Article  CAS  Google Scholar 

  • Sautebin L, Ialenti A, Ianaro A, Di Rosa M (1998) Relationship between nitric oxide and prostaglandins in carrageenin pleurisy. Biochem Pharmacol 55:1113–1117

    Article  CAS  Google Scholar 

  • Shahid F, Wanasundara PKJPD (1992) Phenolic antioxidants. Crit Rev Food Sci Nutr 32:67–103

    Article  Google Scholar 

  • Singh VK, Mehrotra S, Narayan P, Pandey CM, Agarwal SS (2000) Modulation of autoimmune diseases by nitric oxide. J Immunol Res 22:1–19

    Article  CAS  Google Scholar 

  • Singleton V, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Amer J Enol Viti 16:144–158

    CAS  Google Scholar 

  • Spelman K, Burns JJ, Nichols D, Winters N, Ottersberg S, Tenborg M (2006) Modulation of cytokine expression by traditional medicines: a review of herbal immunomodulators. Altern Med Rev 11(2):128–150

    PubMed  Google Scholar 

  • Sugihara N, Arakawa T, Ohnishi M, Furuno K (1999) Anti and pro-oxidative effects of flavonoids on metal induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes located with α-linolenic acid. Free Radical Biol Med 27:1313–1323

    Article  CAS  Google Scholar 

  • Wang L, Xu ML, Hu JH, Rasmussen SK, Wang MH (2011) Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells - Involvement of ROS generation and polyamine depletion. Food Chem Toxicol 49(1):149–154

    Article  CAS  Google Scholar 

  • Willeaume V, Kruys V, Mijatovic T, Huez G (1996) Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. J Inflamm 46:1–12

    CAS  Google Scholar 

  • Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I (1998) Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl-2-picrylhydrazyl radical. Biochem Phamacol 56:213–222

    Article  CAS  Google Scholar 

  • Zhao F, Wang L, Liu K (2009) In vitro anti-inflammatory effects of arctigenin, a lignan from Arctium lappa L., through inhibition on iNOS pathway. J Ethnopharacol 122:457–462

    Article  CAS  Google Scholar 

  • Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

    Article  CAS  Google Scholar 

  • Zhou K, Yu L (2006) Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT-Food Sci Technol 39(10):1155–1162

    Article  CAS  Google Scholar 

  • Zhu N, Wang M, Wei GJ, Lin JK, Yang CS, Ho CT (2001) Identification of reaction products of (-)-epigallocatechin, (-)-epigallocatechin gallate and pyrogallol with 2,2-diphenyl-1- picrylhydrazyl radical. Food Chem 73:345–349

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through High Value-added Food Technology Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (Grant No. 114036-04-4-SB010).

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Authors and Affiliations

  1. WELLPHYTO Co. Ltd, Room 415, K1 Office town, Chipyeong-ro 124, Gwangju, 61950, Republic of Korea

    Hee-Ock Boo & Jeong-Hun Park

  2. Faculty of Food Nutrition and Cookery, Woosong College, Daejeon, 34606, Republic of Korea

    Hag-Hyun Kim & Soo-Jeong Kwon

  3. Department of Crop Science, Chungbuk National University, Cheongju, 28644, Republic of Korea

    Sun-Hee Woo

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  1. Hee-Ock Boo
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  2. Jeong-Hun Park
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Correspondence to Hee-Ock Boo.

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Boo, HO., Park, JH., Kim, HH. et al. Effect of extraction solvent on in vitro anti-inflammatory, antioxidant activity, total phenol and flavonoid contents in Codonopsis lanceolata. J. Crop Sci. Biotechnol. 24, 127–136 (2021). https://doi.org/10.1007/s12892-020-00062-5

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  • DOI: https://doi.org/10.1007/s12892-020-00062-5

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