Abstract
The assembly of superior varieties and collection of rice germplasm involves the process of selecting and storing elders that have superior genotypic properties and phenotypes. The anther culture techniques on indica black rice cultivar have a high difficulty factor to get plants, because of the low regeneration ability at the plant formation phase from the anther callus. This study aimed to investigate the influence of the cold-pretreatment time on anther, the combination of plant growth regulators (PGR’s) concentrations, and putrescine concentrations in media for the increase callus induction and plant regeneration of indica black rice. The optimization of the cold pre-treatment time was important to obtain the high-frequency callus induction, which showed that anther at the 4°C for 8 days formed the high callus induction (20%). To accelerate the callus induction, the application of 20 µM putrescine in the MS medium could produce more friable embryogenic callus for 24 days with 27% of callus formation. Generally, the optimal medium for the high frequency of callus induction contained 2 mgL−1 NAA+0.5 mgL−1 Kinetin+20 µM putrescine. Especially indica black rice cultivars, the best media to get a high plant regeneration frequency were N6 media containing the combination of 2 mgL−1 IAA and 2,5 mgL−1 Kinetin. The total callus regenerated to plantlet about 12.5%. The study of the callus induction and in-vitro plant regeneration medium for indica black rice were still important to develop to get the best result for other cultivars.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Afza, R., Shen, M., Zapata-Arias, F. J., Xie, J., Fundi, H. K., Lee, K. S., Kodym, A. (2000). Effect of spikelet position on rice anther culture efficiency. Plant Science, 153(2), 155–159. https://doi.org/10.1016/S0168-9452(99)00266-6
Bishnoi, U., Jain, R. K., Rohilla, J. S., Chowdhury, V. K., Gupta, K. R., & Chowdhury, J. B. (2000). Anther culture of recalcitrant indica x Basmati rice hybrids: Anther culture of indica rice hybrids. Euphytica, 114(2), 93–101. https://doi.org/10.1023/A:1003915331143
Bolea, C. A., & Vizireanu, C. (2017). Polyphenolic content and antioxidant properties of black rice flour. Annals of the University Dunarea de Jos of Galati, Fascicle VI: Food Technology, 41(2), 75–85.
Bouchereau, A., Aziz, A., Larher, F., & Martin-Tanguy, J. (1999). Polyamines and environmental challenges: Recent development. Plant Science, 140(2), 103–125. https://doi.org/10.1016/S0168-9452(98)00218-0
Chaleff, R. S., & Stolarz, A. (1981). Factors influencing the frequency of callus formation among cultured rice (Oryza sativa) anthers. Physiologia Plantarum, 51(2), 201–206. https://doi.org/10.1111/j.1399-3054.1981.tb02699.x
Chiancone, B., Tassoni, A., Bagni, N., & Germanà, M. A. (2006). Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan. Plant Cell, Tissue and Organ Culture, 87(2), 145–153. https://doi.org/10.1007/s11240-006-9149-4
Cohen, S. (1998). A Guide to the Polyamines. Oxford University Press, USA.
Dewi, I. S., & Purwoko, B. S. (2016a). Kultur Antera untuk Percepatan Perakitan Varietas Padi di Indonesia. Jurnal AgroBiogen, 8(2), 78. https://doi.org/10.21082/jbio.v8n2.2012.p78-88
Dewi, I. S., & Purwoko, B. S. (2016b). Role of Polyamines in Inhibition of Ethylene Biosynthesis and Their Effects on Rice Anther Culture Development. Indonesian Journal of Agricultural Science, 9(2), 60. https://doi.org/10.21082/ijas.v9n2.2008.p60-67
Dewi, I. S., Purwoko, B. S., & Aswidinnoor, H. (2004). Kultur antera padi pada beberapa formulasi media yang mengandung poliamin. Jurnal Bioteknologi Pertanian, 9(1), 14–19.
Górecka, K., Cvikrová, M., Kowalska, U., Eder, J., Szafrańska, K., Górecki, R., & Janas, K. M. (2007). The impact of Cu treatment on phenolic and polyamine levels in plant material regenerated from embryos obtained in anther culture of carrot. Plant Physiology and Biochemistry, 45(1), 54–61. https://doi.org/10.1016/j.plaphy.2006.12.007
Gueye, T., & Ndir, K. N. (2010). In vitro production of double haploid plants from two rice species (oryza sativa L. and oryza glaberrima steudt.) for the rapid development of new breeding material. Scientific Research and Essays, 5(7), 709–713.
He, T., Yang, Y., Tu, S. B., Yu, M. Q., & Li, X. F. (2006). Selection of interspecific hybrids for anther culture of indica rice. Plant Cell, Tissue and Organ Culture, 86(2), 271–277. https://doi.org/10.1007/s11240-006-9117-z
Hema, B. P., & Murthy, H. N. (2008). Improvement of in vitro androgenesis in niger using amino acids and polyamines. Biologia Plantarum, 52(1), 121–125. https://doi.org/10.1007/s10535-008-0024-5
Herath, H., Bandara, D., & Samarajeewa, P. (2010). Effect of Culture Media for Anther Culture of Indica Rice Varieties and Hybrids of Indica and Japonica. Tropical Agricultural Research and Extension, 10(0), 17. https://doi.org/10.4038/tare.v10i0.1866
Ichikawa, H., Ichiyanagi, T., Xu, B., Yoshii, Y., Nakajima, M., & Konishi, T. (2001). Antioxidant Activity of Anthocyanin Extract from Purple Black Rice. Journal of Medicinal Food, 4(4), 211–218. https://doi.org/10.1089/10966200152744481
Jang, H. H., Park, M. Y., Kim, H. W., Lee, Y. M., Hwang, K. A., Park, J. H., & Kwon, O. (2012). Black rice (Oryza sativa L.) extract attenuates hepatic steatosis in C57BL/6 J mice fed a high-fat diet via fatty acid oxidation. Nutrition and Metabolism, 9(1), 27. https://doi.org/10.1186/1743-7075-9-27
Kaushal, L., Balachandran, S. M., Ulaganathan, K., & Shenoy, V. (2014). Effect of culture media on improving anther culture response of rice (Oryza sativa L.). International Journal of Agriculture Innovations and Research, 3(1), 218–224.
Kiviharju, E., & Pehu, E. (1998). The effect of cold and heat pretreatments on anther culture response of Avena sativa and A. sterilis. Plant Cell, Tissue and Organ Culture, 54(2), 97–104. https://doi.org/10.1023/A:1006167306638
Kumar, A., Altabella, T., Taylor, M. A., & Tiburcio, A. F. (1997). Recent advances in polyamine research. Trends in Plant Science, 2(4), 124–130. https://doi.org/10.1016/S1360-1385(97)01013-3
Lestari, A. P. (2008). Evaluasi mutu beras 18 galur padi hasil kultur anter. Seminar Nasional Padi, 1449–1455.
Manonmani, S., & Khan, A. (2004). Effect of growth regulators on androgenesis and regeneration in rice (Oryza sativa L.). The Indian Journal of Genetics and Plant Breeding, 64(1), 17–20.
Martin-Tanguy, J. (2001). Metabolism and function of polyamines in plants: Recent development (new approaches). Plant Growth Regulation, 34(1), 135–148. https://doi.org/10.1023/A:1013343106574
Mayakaduwa, D. M. R. G., & Silva, T. D. (2018). Anther Culture as a Supplementary Tool for Rice Breeding. Rice Crop — Current Developments, 1–16. https://doi.org/10.5772/intechopen.76157
Mishra, R., & Rao, G. J. N. (2016). In-vitro Androgenesis in Rice: Advantages, Constraints and Future Prospects. Rice Science, 23(2), 57–68. https://doi.org/10.1016/j.rsci.2016.02.001
Pape, H. C., Remmers, D., Rice, J., Ebisch, M., Krettek, C., & Tscherne, H. (2000). Appraisal of early evaluation of blunt chest trauma: development of a standardized scoring system for initial clinical decision making. The Journal of Trauma, 49(3), 496–504. https://doi.org/10.1097/00005373-200009000-00018
Rukmini, M., Rao, G. J. N., & Rao, R. N. (2013). Effect of cold pretreatment and phytohormones on anther culture efficiency of two indica rice (Oryza sativa L.) hybrids-Ajay and Rajalaxmi. Journal of Experimental Biology and Agricultural Sciences, 1(2), 69–76. Retrieved from http://www.jebas.org/vol1i2/RukminietalJEBAS.pdf
Sasmita, P. (2010). Evaluation of uniformity, variability, and stability of agronomic traits of doubledd haploid rice lines resulting from anther culture. Nusantara Bioscience, 2(2), 67–72. https://doi.org/10.13057/nusbiosci/n020203
Sen, C., & Singh, R. P. (2011). Anther culture response in boro hybrids.pdf. Asian Journal of Biotechnology, 3(5), 470–477.
Shukla, R., & Ojha, E. P. K. M. D. (2018). Best plant harmon combination for In vitro callus initiation, organogenesis and regeneration of rice cv. swarna sub1. Journal of Pharmacognosy and Phytochemistry, 7(2), 1890–1894.
Silva, T., & Ratnayake, W. (2011). Anther Culture Potential of Indica Rice Varieties, Kurulu Thuda and BG 250. Tropical Agricultural Research and Extension, 12(2), 53. https://doi.org/10.4038/tare.v12i2.2789
Sompong, R., Siebenhandl-Ehn, S., Linsberger-Martin, G., & Berghofer, E. (2011). Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka. Food Chemistry, 124(1), 132–140. https://doi.org/10.1016/j.foodchem.2010.05.115
Szafrańska, K., Cvikrová, M., Kowalska, U., Górecka, K., Górecki, R., Martincová, O., & Janas, K. M. (2011). Influence of copper ions on growth, lipid peroxidation, and proline and polyamines content in carrot rosettes obtained from anther culture. Acta Physiologiae Plantarum, 33(3), 851–859. https://doi.org/10.1007/s11738-010-0610-y
Tiainen, T. (1996). Influence of ethylene in microspore embryogenesis. 1, 177–187. https://doi.org/10.1007/978-94-017-1860-8_10
Tiburcio, A. F., Campos, J. L., Figueras, X., & Besford, R. T. (1993). Recent advances in the understanding of polyamine functions during plant development. Plant Growth Regulation, 12(3), 331–340. https://doi.org/10.1007/BF00027215
Trejo-Tapia, G., Maldonado Amaya, U., Salcedo Morales, G., De Jesús Sánchez, A., Martínez Bonfil, B., Rodríguez-Monroy, M., & Jiménez-Aparicio, A. (2002). The effects of cold-pretreatment, auxins and carbon source on anther culture of rice. Plant Cell, Tissue and Organ Culture, 71(1), 41–46. https://doi.org/10.1023/A:1016558025840
Tripathy, S. K., Swain, D., Mohapatra, P. M., Prusti, A. M., Sahoo, B., Panda, S., & Behera, S. K. (2019). Exploring factors affecting anther culture in rice (Oryza sativa L.). Journal of Applied Biology & Biotechnology, 7(2), 87–92. https://doi.org/10.7324/jabb.2019.70216
Xie, J., Gao, M., Cai, Q., Cheng, X., Shen, Y., & Liang, Z. (1995). Improved isolated microspore culture efficiency in medium with maltose and optimized growth regulator combination in japonica rice (Oryza sativa). Plant Cell, Tissue and Organ Culture, 42(3), 245–250. https://doi.org/10.1007/BF00029994
Yawadio, R., Tanimori, S., & Morita, N. (2007). Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities. Food Chemistry, 101(4), 1616–1625. https://doi.org/10.1016/j.foodchem.2006.04.016
Zapata-Arias, F. J., Torrizo, L. B., & Ando, A. (1995). Current developments in plant biotechnology for genetic improvement: the case of rice (Oryza sativa L.). World Journal of Microbiology & Biotechnology, 11(4), 393–399. https://doi.org/10.1007/BF00364614
Acknowledgements
This research was supported by the Ministry of Research, Technology and Higher Education of the Republic of Indonesia, Directorate General Strengthening Research and Development, Directorate of Research and Community Service, Magister Thesis Research Grant Num: 061/SP2H/LT/DRPM/2019. Great thank to Plant Molecular Breeding (PMB) laboratory, Kyungpook National University for the supporting facilities in this research.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Consent for publication
Not aplicable.
Ethics approval and consent to participate
Not aplicable.
Funding
The Ministry of Research, Technology and Higher Education of the Republic of Indonesia, Directorate General Strengthening Research and Development, Directorate of Research and Community Service, Magister Thesis Research Grant Num: 061/SP2H/LT/DRPM/2019.
Availability of data and materials
All datasets and software used to support the conclusions of this article are available and can be accessed through correspondents.
Author’s contributions statement
A. Maharani: performed cultivation, callus induction, and data analysis.
W.I.D. Fanata: second supervisor and contributed to the dicussion.
F.N. Laeli: performed the plant regeneration and data analysis.
K.M. Kim: supported facilities during research in his laboratory and contributed to the discussion, kkm@knu.ac.kr.
T. Handoyo: performed experiments, contributed to the discussion, corresponding author and supervising professor of A. Maharani and F.N. Laeli, trihandoyo.faperta@unej.ac.id
Competing interests
The authors declare that they have no competing interests.
Novelty statement
Indonesia is one of the countries that have a lot of germplasm and produces rice in the most worldwide. Especially, black rice still has great potential for the development of antioxidant-rich rice new varieties that have high economic value on the world market. Indica black rice is a recalcitrant seed type, it was very difficult to callus induction and plant regeneration. However, the successfully of anther culture technique depend on some factors, including anther cold pre-treatment, plant growth regulators and polyamine. the Anther culture technique is an alternative to the development of new black rice varieties to obtain plants with high homozygosity thread. This study aimed to investigate the influence of the cold-pretreatment time on anther, the combination of plant growth regulators (PGR’s) concentrations, and putrescine concentrations in media for the increase callus induction and plant regeneration of indica black rice.
Abbreviations
PGR’s, Plant Growth Regulators; Put, Putrescine; Kin, Kinetin; NAA, Naphtalene Acetic Acid, IAA, Indole Acetic Acid; HB, Hitam Bantul; dap, days after planting
Rights and permissions
About this article
Cite this article
Maharani, A., Fanata, W.I.D., Laeli, F.N. et al. Callus Induction and Regeneration from Anther Cultures of Indonesian Indica Black Rice Cultivar. J. Crop Sci. Biotechnol. 23, 21–28 (2020). https://doi.org/10.1007/s12892-019-0322-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12892-019-0322-0