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Study of Some Toxicological Aspects of Titanium Dioxide Nanoparticles Through Oxidative Stress, Genotoxicity, and Histopathology in Tilapia, Oreochromis mossambicus

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Abstract

Extensive use of nanotechnology in multiple commodities is raising concerns about nanotoxicity. In particular, many studies have reported the health-hazardous effects of titanium dioxide nanoparticles (TiO2-NPs). The present study focuses on the toxicity and accumulation of TiO2-NPs in tilapia (Oreochromis mossambicus). For this purpose, tilapia was kept in water tanks, acclimatized for 14 days, and treated with different doses of TiO2 nanoparticles: 0, 0.5, 1.0, and 1.5 mg TiO2-NPs/L. Results revealed an increase in the accumulation of TiO2-NPs with an increase in doses. Moreover, with a higher dose (1.5 mg/L), gills had maximum levels compared to muscles and liver tissues, whereas other doses showed different accumulation patterns. Catalase, glutathione, and lipid peroxidation levels were significantly higher in the gills, and superoxide dismutase levels were significantly higher in the liver. Characteristics like thickening and fusion in lamellae, rupturing of gill filaments, and hyperplasia of gills were also recorded. The phenomenon of increased necrosis and apoptosis in the liver was also noticed with increasing concentration of TiO2-NPs along with the formation of sinusoid spaces and condensed nuclear bodies. Elevated values of olive tail movement and % tail DNA were also noticed with an increased concentration of TiO2-NPs. This study concluded that TiO2-NPs produced oxidative stress by accumulation in soft tissues and induced pathology and genotoxicity.

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References

  1. Masciangioli, T., & Zhang, W.-X. (2003). Peer reviewed: environmental technologies at the nanoscale. Environmental Science and Technology ACS Publications, 37(5), 102A–108A.

  2. Asghar, M. S., Quershi, N. A., Jabeen, F., Shakeel, M., & Khan, M. (2016). Genotoxicity and oxidative stress analysis in the Catla catla treated with ZnO NPs. Journal of Biodiversity and Environmental Sciences, 8, 91–104.

    Google Scholar 

  3. Kakakhel, M. A., Wu, F., Feng, H., Hassan, Z., Ali, I., Saif, I., ... & Wang, W. (2021). Biological synthesis of silver nanoparticles using animal blood, their preventive efficiency of bacterial species, and ecotoxicity in common carp fish. Microscopy Research and Technique, 84(8), 1765-1774

  4. Kakakhel, M. A., Wu, F., Sajjad, W., Zhang, Q., Khan, I., Ullah, K., & Wang, W. (2021). Long-term exposure to high-concentration silver nanoparticles induced toxicity, fatality, bioaccumulation, and histological alteration in fish (Cyprinus carpio). Environmental Sciences Europe, 33(1), 1–11.

    Article  Google Scholar 

  5. Khan, M. S., Qureshi, N. A., & Jabeen, F. (2017). Assessment of toxicity in fresh water fish Labeo rohita treated with silver nanoparticles. Applied Nanoscience, 7, 167–179.

    Article  Google Scholar 

  6. Khan, M. S., Jabeen, F., Qureshi, N. A., Asghar, M. S., Shakeel, M., & Noureen, A. (2015). Toxicity of silver nanoparticles in fish: A critical review. Journal of Biological and Environmental Sciences, 6, 211–227.

    Google Scholar 

  7. Raza, A., Javed, S., Qureshi, M. Z., & Khan, M. S. (2017). Synthesis and study of catalytic application of l-methionine protected gold nanoparticles. Applied Nanoscience, 7, 429–437.

    Article  Google Scholar 

  8. Ramsden, C. S., Henry, T. B., & Handy, R. D. (2013). Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish. Aquatic Toxicology, 126, 404–413.

    Article  Google Scholar 

  9. Asghar, M. S., Qureshi, N. A., Jabeen, F., Khan, M. S., Shakeel, M., & Noureen, A. (2015). Toxicity of zinc nanoparticles in fish: A critical review. Journal of Biodiversity and Environmental Sciences, 7, 431–439.

    Google Scholar 

  10. Khan, M. S., Qureshi, N. A., Jabeen, F., Wajid, M., Sabri, S., & Shakir, M. (2020). The role of garlic oil in the amelioration of oxidative stress and tissue damage in rohu Labeo rohita treated with silver nanoparticles. Fisheries Science. 1–15

  11. Khan, M. S., Qureshi, N. A., & Jabeen, F. (2018). Ameliorative role of nano-ceria against amine coated Ag-NP induced toxicity in Labeo rohita. Applied Nanoscience, 8, 323–337.

    Article  Google Scholar 

  12. Hao, L., Wang, Z., & Xing, B. (2009). Effect of sub-acute exposure to TiO2 nanoparticles on oxidative stress and histopathological changes in Juvenile Carp (Cyprinus carpio). Journal of Environmental Sciences, 21, 1459–1466.

    Article  Google Scholar 

  13. Shakeel, M., Jabeen, F., Shabbir, S., Asghar, M. S., Khan, M. S., & Chaudhry, A. S. (2016). Toxicity of nano-titanium dioxide (TiO 2-NP) through various routes of exposure: A review. Biological Trace Element Research, 172, 1–36.

    Article  Google Scholar 

  14. Asghar, M. S., Qureshi, N. A., Jabeen, F., Khan, M. S., Shakeel, M., & Chaudhry, A. S. (2018). Ameliorative effects of Selenium in ZnO NP-induced oxidative stress and hematological alterations in Catla catla. Biological Trace Element Research, 186, 279–287.

    Article  Google Scholar 

  15. Shakeel, M., Jabeen, F., Iqbal, R., Chaudhry, A. S., Zafar, S., Ali, M., Khan, M. S., Khalid, A., Shabbir, S., & Asghar, M. S. (2018). Assessment of titanium dioxide nanoparticles (TiO 2-NPs) Induced hepatotoxicity and ameliorative effects of Cinnamomum cassia in Sprague-Dawley rats. Biological Trace Element Research, 182, 57–69.

    Article  Google Scholar 

  16. Rocco, L., Santonastaso, M., Mottola, F., Costagliola, D., Suero, T., Pacifico, S., & Stingo, V. (2015). Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio. Ecotoxicology and Environmental Safety, 113, 223–230.

    Article  Google Scholar 

  17. Khan, M. S., Qureshi, N. A., Jabeen, F., Asghar, M. S., Shakeel, M., & Fakhar-e-Alam, M. (2017). Eco-friendly synthesis of silver nanoparticles through economical methods and assessment of toxicity through oxidative stress analysis in the Labeo Rohita. Biological Trace Element Research, 176, 416–428.

    Article  Google Scholar 

  18. Griffitt, R. J., Hyndman, K., Denslow, N. D., & Barber, D. S. (2009). Comparison of molecular and histological changes in zebrafish gills exposed to metallic nanoparticles. Toxicological Sciences, 107, 404–415.

    Article  Google Scholar 

  19. Tang, T., Zhang, Z., & Zhu, X. (2019). Toxic effects of TiO2 NPs on zebrafish. International Journal of Environmental Research and Public Health, 16, 523.

    Article  Google Scholar 

  20. Zhu, X., Zhu, L., Duan, Z., Qi, R., Li, Y., & Lang, Y. (2008). Comparative toxicity of several metal oxide nanoparticle aqueous suspensions to Zebrafish (Danio rerio) early developmental stage. Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances and Environmental Engineering 43, 278–284.

    Google Scholar 

  21. Nigro, M., Bernardeschi, M., Costagliola, D., Della Torre, C., Frenzilli, G., Guidi, P., Lucchesi, P., Mottola, F., Santonastaso, M., & Scarcelli, V. (2015). n-TiO2 and CdCl2 co-exposure to titanium dioxide nanoparticles and cadmium: Genomic, DNA and chromosomal damage evaluation in the marine fish European sea bass (Dicentrarchus labrax). Aquatic Toxicology, 168, 72–77.

    Article  Google Scholar 

  22. Buege, J. A., & Aust, S. D. (1978). In: Methods in enzymology, Elsevier, Vol. 52, pp. 302-310.

  23. Claiborne, A. (1985). Handbook of methods for oxygen radical research. CRC Press.

    Google Scholar 

  24. Marklund, S., & Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry, 47, 469–474.

    Article  Google Scholar 

  25. Jollow, D., Mitchell, J., Zampaglione, N., & Gillette, J. J. P. (1974). Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology, 11, 151–169.

    Article  Google Scholar 

  26. Ates, M., Demir, V., Adiguzel, R., & Arslan, Z. (2013). Bioaccumulation, subacute toxicity, and tissue distribution of engineered titanium dioxide nanoparticles in goldfish (Carassius auratus). Journal of Nanomaterials. 2013

  27. Sohail, M., Khan, M. N., Chaudhry, A. S., & Qureshi, N. A. (2016). Bioaccumulation of heavy metals and analysis of mineral element alongside proximate composition in foot, gills and mantle of freshwater mussels (Anodonta anatina). Rendiconti Lincei, 27, 687–696.

    Article  Google Scholar 

  28. Wilhelm, J. (1990). Metabolic aspects of membrane lipid peroxidation. Acta Universitatis Carolinae. Medica. Monographia., 137, 1–53.

    Google Scholar 

  29. Asghar, M. S., Khan, M. S., Aziz, N., Qurashi, F. J. Wajid, M. & Farooq, Z. (2017). Antioxidant potential of Allium sativum, Cinnamomum zeylanicum and Azadirachta indica against free radicals and their antimicrobial activity against isolated microbes from diseased Tilapia. Journal of Entomology and Zoology Studies

  30. Khan, M. S., Jabeen, F., Asghar, M. S., Qureshi, N., Shakeel, M., Noureen, A., & Shabbir, S. (2015). Role of nao-ceria in the amelioration of oxidative stress: Current and future applications in medicine. International Journal of Biosciences (IJB), 6, 89–109.

    Article  Google Scholar 

  31. Khan, M. U., Qurashi, N. A., Khan, M. S., Jabeen, F., Umar, A., Yaqoob, J., & Wajid, M. (2016). Generation of reactive oxygen species and their impact on the health related parameters: A critical review. International Journal of Biosciences, 9, 303–323.

    Google Scholar 

  32. Hamid, A., Khan, M. U., Yaqoob, J., Umar, A., Rehman, A., Javed, S., Sohail, A., Anwar, A., Khan, M., & Ali, A. (2016). Assessment of mercury load in river Ravi, urban sewage streams of Lahore Pakistan and its impact on the oxidative stress of exposed fish. Journal of Biological and Environmental Sciences, 8, 63–72.

    Google Scholar 

  33. Xiong, D., Fang, T., Yu, L., Sima, X., & Zhu, W. (2011). Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: Acute toxicity, oxidative stress and oxidative damage. Science of the Total Environment, 409, 1444–1452.

    Article  Google Scholar 

  34. Smith, C. J., Shaw, B. J., & Handy, R. D. (2007). Toxicity of single walled carbon nanotubes to rainbow trout,(Oncorhynchus mykiss): Respiratory toxicity, organ pathologies, and other physiological effects. Aquatic Toxicology, 82, 94–109.

    Article  Google Scholar 

  35. Ahmad, S., Gull, M., Shah, A., Khan, F. U., Rafiq, M., Lutfullah, G., Khan, A. Z., Amin, F., Azhar, N., & Khan, M. S. (2019). Antimicrobial, antioxidant and cytotoxic potential of aerial parts of Monotheca buxifolia. Journal of Mathematical and Fundamental Sciences, 51, 138–151.

    Article  Google Scholar 

  36. Guan, H., & Lin, Y. (2004). Constitution and the tolerance of several heavy metals on the gill tissue of carp. Chinese Journal of Fisheries, 17, 68–72.

    Google Scholar 

  37. Rahman, Q., Lohani, M., Dopp, E., Pemsel, H., Jonas, L., Weiss, D. G., & Schiffmann, D. (2002). Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environmental Health Perspectives, 110, 797–800.

    Article  Google Scholar 

  38. Vignardi, C. P., Hasue, F. M., Sartório, P. V., Cardoso, C. M., Machado, A. S., Passos, M. J., Santos, T. C., Nucci, J. M., Hewer, T. L., & Watanabe, I.-S. (2015). Genotoxicity, potential cytotoxicity and cell uptake of titanium dioxide nanoparticles in the marine fish Trachinotus carolinus (Linnaeus, 1766). Aquatic Toxicology, 158, 218–229.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Zoology, University of the Punjab, Lahore, Pakistan

    Khurram Shahzad, Muhammad Naeem Khan & Chaman Ara

  2. Department of Zoology, University of Okara, Okara, 56130, Pakistan

    Khurram Shahzad, Muhammad Khalil Ahmad Khan & Muhammad Saleem Khan

  3. School of Agriculture, Natural and Environmental Sciences, Newcastle University, Newcastle, NE1 7RU, UK

    Abdul Shakoor Chaudhry

  4. Department of Zoology, Government College University Faisalabad, Faisalabad, Pakistan

    Farhat Jabeen

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  1. Khurram Shahzad
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Correspondence to Muhammad Saleem Khan.

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Shahzad, K., Khan, M.N., Jabeen, F. et al. Study of Some Toxicological Aspects of Titanium Dioxide Nanoparticles Through Oxidative Stress, Genotoxicity, and Histopathology in Tilapia, Oreochromis mossambicus. BioNanoSci. 12, 1116–1124 (2022). https://doi.org/10.1007/s12668-022-01024-7

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