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Comparison of the microbial community composition of pristine rock cores and technical influenced well fluids from the Ketzin pilot site for CO2 storage

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Abstract

Two geological formations at the CO2 storage pilot site in Ketzin (Germany) were geochemically and microbiologically characterized to further evaluate changes resulting from CO2 injection. Well fluids were collected from both Stuttgart (storage formation, ~650 m depth) and Exter Formations (~400 m depth, overlying the caprock) either through pump tests or downhole samplings. Rock samples were retrieved during a deep drilling into the Exter Formation and primarily comprised quartz, ferrous dolomite or ankerite, calcite, analcime, plagioclase and clay minerals, as determined through X-ray diffraction analyses. In the rocks, the total organic carbon (TOC), which potentially contributes to microbial growth, was mostly below 1000 mg kg−1. The geochemical characterization of fluids revealed significant differences in the ionic composition between both formations. The microbial characterization was performed through fluorescence in situ hybridization and 16S rRNA gene fingerprinting. In the fluids obtained from the Stuttgart Formation, the microbial activity was affected by the relatively high TOC, introduced by the organic drill mud. The total cell counts were approximately 106 cells mL−1. The microbial community was characteristic of a saline deep biosphere environment enriched through increased carbon availability, with sulfate-reducing bacteria as the most abundant microorganisms (up to 60 % of total cells). Species belonging to halophilic/halotolerant Proteobacteria and Firmicutes were primarily detected. In Exter Formation rocks, Proteobacteria and Actinobacteria were detected. These data provide an explicit reference to further evaluate environmental changes and community shifts in the reservoir during CO2 storage and provide information for evaluating the storage efficiency and reliability.

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Acknowledgments

We would like to thank all partners in the Ketzin projects and CO2 storage center for their continued support and contributions. Special thanks go to EEW (Erdoel-Erdgas Workover GmbH) and Dr. Andrea Seibt (BWG) for their most valuable advice and operational support during the downhole sampling and to Dr. Maren Wandrey for her support during the pump test at the Ktzi 202 well and for the corresponding fluorescein measurements. Dr. Hannah Halm and Dr. Tobias Lienen (GFZ German Research Centre for Geosciences, Potsdam) are acknowledged for critical reading of the manuscript. This research was funded by the European Commission, the Federal Ministry of Economics and Technology and the Federal Ministry of Education and Research in the frame of the CO2SINK project and by the Federal Ministry for Education and Research within the GEOTECHNOLOGIEN Program in the framework of CO2MAN (CO2 Reservoir Management 03G0760A-F).

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    Authors and Affiliations

    1. Section 5.3 Geomicrobiology, GFZ German Research Centre for Geosciences, 14473, Telegrafenberg, Potsdam, Germany

      Linda Pellizzari, Daria Morozova, Dominik Neumann, Monika Kasina & Hilke Würdemann

    2. Institute of Geological Sciences, Jagiellonian University, 30-063, Krakow, Poland

      Monika Kasina

    3. Section 6.2 Geothermal Energy Systems, GFZ German Research Centre for Geosciences, 14473, Telegrafenberg, Potsdam, Germany

      Stefan Klapperer

    4. DEA Deutsche Erdoel AG Corporate QHSSE, Ueberseering 40, 22297, Hamburg, Germany

      Michael Zettlitzer

    5. Department of Engineering and Natural Sciences, Merseburg University of Applied Sciences, 06217, Merseburg, Germany

      Hilke Würdemann

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    6. Michael Zettlitzer
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    7. Hilke Würdemann
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    Correspondence to Hilke Würdemann.

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    Linda Pellizzari and Daria Morozova have contributed to the manuscript equally.

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    Pellizzari, L., Morozova, D., Neumann, D. et al. Comparison of the microbial community composition of pristine rock cores and technical influenced well fluids from the Ketzin pilot site for CO2 storage. Environ Earth Sci 75, 1323 (2016). https://doi.org/10.1007/s12665-016-6111-6

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