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Soil C turnover, microbial biomass and respiration, and enzymatic activities following rangeland conversion to wheat–alfalfa cropping in a semi-arid climate

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Abstract

Conversion from rangeland to cropland potentially influences soil organic matter (SOM) dynamics and biochemical properties. The purpose of the current study was to assess changes in soil properties and investigate the interaction between SOM and enzyme activities following cultivation of native rangelands for more than 40 years. The quantities of soil organic carbon (C) and microbial biomass C (MBC), aggregate stability, microbial respiration rate (MRR), potential C mineralization (PCM) and the activities of soil enzymes involved in C, N, P and S cycling at the 0–20 and 20–40 cm depths were evaluated in never-cultivated natural rangelands and their corresponding farmlands with similar soil type and climatic conditions at Dehnow located in Central west, Iran. Cultivation in native rangelands resulted in a significant decline of most soil properties in farmlands, depending on soil sampling depths. Conversion of rangelands to their farmland counterparts decreased soil C (50–66 %), MBC (48–61 %), MRR (20–44 %), PCM (45–59 %), the activities of urease (5–17 %), invertase (7–28 %) and arylsulfatase (17–20 %) per soil mass, while increased C turnover rate (61–63 %), microbial metabolic quotient (qCO2) values (41–54 %) and enzyme activities per unit C and MBC (61–190 %) without affecting microbial quotient (MBC/C ratio) values and the activities of soil phosphatases per soil mass. The decreases in soil microbial and biochemical properties were largely the result of a substantial decline in soil C contents and aggregate stability by tillage activities in farmlands. Although the absolute activities of soil enzymes (i.e., activity per dry soil mass) showed inconsistent responses to land use changes and were even less responsive than soil organic C and MBC contents to changing land uses, the specific enzyme activities expressed either per unit of C or MBC tended to increase considerably and consistently with this conversion and were more sensitive than soil C and MBC contents. Soil C turnover rates and metabolic quotients were found to correlate positively with the specific enzyme activities, indicating an interaction between SOM and enzyme activities. The specific enzymatic activity could be an appropriate and integrative simple index to (1) assess soil quality changes, (2) detect changes in soil microbial community; and even (3) associate the losses of SOM with the presence of metabolically more active decomposer biota in rangelands cultivated for the long term.

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References

  • Acosta-Martinez V, Zobeck TM, Gill TE, Kennedy AC (2003) Enzyme activities and microbial community structure of agricultural semiarid soils. Biol Fertil Soils 38:216–227

    Article  Google Scholar 

  • Acosta-Martínez V, Cruz L, Sotomayor-Ramírez D, Perez-Alegría L (2007) Enzyme activities as affected by soil properties and land use in a tropical watershed. Appl Soil Ecol 35:35–45

    Article  Google Scholar 

  • Alef K, Nannipieri P (1995) Methods in applied soil microbiology and biochemistry. Academic Press, London

    Google Scholar 

  • Allison SD, Jastrow JD (2006) Activities of extracellular enzymes in physically isolated fractions of restored grassland soils. Soil Biol Biochem 38:3245–3256

    Article  Google Scholar 

  • Allison VJ, Condron LM, Peltzer DA, Richardson SJ, Turner BL (2007) Changes in enzyme activities and soil microbial community composition along carbon and nutrient gradients at the Franz Josef chronosequence, New Zealand. Soil Biol Biochem 39:1770–1781

    Article  Google Scholar 

  • Alvarez R, Alvarez CR (2000) Soil organic matter pools and their associations with carbon mineralization kinetics. Soil Sci Soc Am J 64:184–189

    Article  Google Scholar 

  • Anderson TH, Domsch KH (1989) Ratios of microbial biomass carbon to total organic-C in arable soils. Soil Biol Biochem 21:471–479

    Article  Google Scholar 

  • Anderson TH, Domsch KH (2010) Soil microbial biomass: the eco-physiological approach. Soil Biol Biochem 42:2039–2043

    Article  Google Scholar 

  • Ayoubi A, Emami N, Ghaffari N, Honarjoo N, Sahrawat KL (2014) Pasture degradation effects on soil quality indicators at different hillslope positions in a semiarid region of western Iran. Environ Earth Sci 71:375–381

    Article  Google Scholar 

  • Azadi H, van den Berg J, Shahvali M, Hosseininia G (2009) Sustainable rangeland management using fuzzy logic: a case study in Southwest Iran. Agric Ecosyst Environ 131:193–200

    Article  Google Scholar 

  • Barral MT, Buján E, Devesa R, Iglesias ML, Velasco-Molina M (2007) Comparison of the structural stability of pasture and cultivated soils. Sci Total Environ 378:174–178

    Article  Google Scholar 

  • Bastida F, Zsolnay A, Hernández T, García C (2008) Past, present and future of soil quality indices: a biological perspective. Geoderma 147:159–171

    Article  Google Scholar 

  • Beheshti A, Raiesi F, Golchin A (2012) Soil properties, C fractions and their dynamics in land use conversion from native forests to croplands in northern Iran. Agric Ecosyst Environ 148:121–133

    Article  Google Scholar 

  • Blake GR, Hartge KH (1986) Bulk density. In: Klute A (ed) Methods of soil analysis Part 1, physical and minerological methods. Agron Monogr 9, 2nd edn. ASA and SSSA, Madison, pp 364–367

    Google Scholar 

  • Burns RG, DeForest JL, Marxsen J, Sinsabaugh RL et al (2013) Soil enzymes in a changing environment: current knowledge and future directions. Soil Biol Biochem 58:216–234

    Article  Google Scholar 

  • Cambardella CA, Elliott ET (1993) Carbon and nitrogen distribution in aggregates from cultivated and native grassland soil. Soil Sci Soc Am J 57:1071–1076

    Article  Google Scholar 

  • Caravaca F, Masciandaro G, Ceccanti B (2002) Land use in relation to soil chemical and biochemical properties in a semiarid Mediterranean environment. Soil Till Res 68:23–30

    Article  Google Scholar 

  • Carter MR, Gregorich EG (2008) Soil sampling and methods of analysis. Canadian Society of Soil Science CRC Press, Taylor and Francis Group, Boca Raton

    Google Scholar 

  • Cochran RL, Collins HP, Kennedy A, Bezdicek DF (2007) Soil carbon pools and fluxes after land conversion in a semiarid shrub-steppe ecosystem. Biol Fertil Soils 43:479–489

    Article  Google Scholar 

  • Dick RP (1997) Soil enzyme activities as integrative indicators of soil health. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CAB International, Wallingford, pp 121–156

    Google Scholar 

  • Dinesh R, Chaudhuri SG, Sheeja TE, Shiva KN (2008) Soil microbial activity and biomass is stimulated by leguminous cover crops. J Plant Nutr Soil Sci 172:288–296

    Article  Google Scholar 

  • Ekenler M, Tabatabai MA (2002) β-Glucosaminidase activity of soils: effect of cropping systems and its relationship to nitrogen mineralization. Biol Fertil Soils 36:367–376

    Article  Google Scholar 

  • Emadi M, Baghernejad M, Memarian HR (2009) Effect of land-use change on soil fertility characteristics within water-stable aggregates of two cultivated soils in northern Iran. Land Use Policy 26:452–457

    Article  Google Scholar 

  • Evrendilek F, Celik I, Kili S (2004) Changes in soil organic carbon and other physical soil properties along adjacent Mediterranean forest, grassland, and cropland ecosystems in Turkey. J Arid Environ 59:743–752

    Article  Google Scholar 

  • Green VS, Stott DE, Cruz JC, Curi N (2007) Tillage impacts on soil biological activity and aggregation in a Brazilian Cerrado Oxisol. Soil Till Res 92:114–121

    Article  Google Scholar 

  • Guo LB, Gifford RM (2002) Soil carbon stocks and land use change: a meta analysis. Glob Change Biol 8:345–360

    Article  Google Scholar 

  • Guo YJ, Ni Y, Han JG (2009) The influence of land use change on chemical and biological properties of steppe soils in northern China. Arid Land Res Manag 23:197–212

    Article  Google Scholar 

  • Gupta VVSR, Germida JJ (1988) Distribution of microbial biomass and its activity in different soil aggregate size classes as affected by cultivation. Soil Biol Biochem 20:777–786

    Article  Google Scholar 

  • Islam KR, Weil RR (2000) Land use effects on soil quality in a tropical forest ecosystem of Bangladesh. Agric Ecosyst Environ 79:9–16

    Article  Google Scholar 

  • Jenkinson DS, Ladd JN (1981) Microbial biomass in soil: measurement and turnover. In: Paul EA, Ladd JN (eds) Soil biochemistry, vol 5. Marcel Dekker, New York, pp 415–471

    Google Scholar 

  • Kandele E, Murer E (1993) Aggregate stability and soil microbial processes in a soil with different cultivation. Geoderma 56:503–513

    Article  Google Scholar 

  • Kandeler E, Eder G (1993) Effect of cattle slurry in grassland on microbial biomass and on activities of various enzymes. Biol Fertil Soils 16:249–254

    Article  Google Scholar 

  • Katsalirou E, Deng S, Nofziger DL, Gerakis A (2010) Long-term management effects on organic C and N pools and activities of C-transforming enzymes in prairie soils. Eur J Soil Biol 46:335–341

    Article  Google Scholar 

  • Kemper DW, Rosenau RC (1986) Aggregate stability and aggregate size distribution. In: Klute A (ed) Methods of soil analysis, Part 1. America Society of Agronomy, WI, pp 425–442

    Google Scholar 

  • Lagomarsino A, Benedetti A, Marinari S, Pompili L, Moscatelli MC, Roggero PP, Lai R, Ledda L, Grego S (2011) Soil organic C variability and microbial functions in a Mediterranean agro-forest ecosystem. Biol Fertil Soils 47:283–291

    Article  Google Scholar 

  • Landi L, Renella G, Moreno JL, Falchini L, Nannipieri P (2000) Influence of cadmium on the metabolic quotient, L- :d-glutamic acid respiration ratio and enzyme activity:microbial biomass ratio under laboratory conditions. Biol Fertil Soils 32:8–16

    Article  Google Scholar 

  • Lee J, Hopmans JW, Rolston DE, Baer SG, Six J (2009) Determining soil carbon stock changes: simple bulk density corrections fail. Agric Ecosyst Environ 134:251–256

    Article  Google Scholar 

  • Li XG, Li FM, Singh B, Zed R, Zhan ZY (2007a) Soil management changes organic carbon pools in alpine pastureland soils. Soil Till Res 93:186–196

    Article  Google Scholar 

  • Li XG, Wang ZF, Ma QF, Li FM (2007b) Crop cultivation and intensive grazing affect organic C pools and aggregate stability in arid grassland soil. Soil Till Res 95:172–181

    Article  Google Scholar 

  • Liu HS, Li LH, Han XG, Huang JH, Sun JX, Wang HY (2006) Respiratory substrate availability plays a crucial role in the response of soil respiration to environmental factors. Appl Soil Ecol 32:284–292

    Article  Google Scholar 

  • Miller M, Dick RP (1995) Dynamics of soil carbon and microbial biomass in whole-soil and aggregates in two cropping systems. Appl Soil Ecol 2:253–261

    Article  Google Scholar 

  • Moscatelli MC, Tizio AD, Marinari S, Grego S (2007) Microbial indicators related to soil carbon in Mediterranean land use systems. Soil Till Res 97:51–59

    Article  Google Scholar 

  • Nannipieri P (2006) Role of stabilised enzymes in microbial ecology and enzyme extraction from soil with potential applications in soil proteomics. In: Nannipieri P, Smalla K (eds) Nucleic acids and proteins in soil, soil biology, vol 8. Springer-Verlag, Berlin Heidelberg, pp 76–94

    Chapter  Google Scholar 

  • Nannipieri P, Sequi P, Fusi P (1996) Humus and enzyme activity. In: Piccolo A (ed) Humic substances in terrestrial ecosystems. Elsevier Science, London, pp 293–328

    Chapter  Google Scholar 

  • Nannipieri P, Kandeler E, Ruggiero P (2002) Enzyme activities and microbiological and biochemical processes in soil. In: Burns RG, Dick RP (eds) Enzymes in the environment: activity ecology and applications. Marcel Dekker, New York, pp 1–33

    Google Scholar 

  • Nannipieri P, Giagnoni L, Renella G, Puglisi E, Ceccanti B, Masciandaro G, Fornasier F, Moscatelli MC, Marinari S (2012) Soil enzymology: classical and molecular approaches. Biol Fertil Soils 48:743–762

    Article  Google Scholar 

  • Piccolo A, Pietramellara G, Mbagwu JSC (1997) Use of humic substances as soil conditioners to increase aggregate stability. Geoderma 75:267–277

    Article  Google Scholar 

  • Qi YC, Dong YS, Liu JY, Domroes M, Geng YB, Liu LX, Liu XR, Yang XH (2007) Effect of the conversion of grassland to spring wheat field on the CO2 emission characteristics in Inner Mongolia, China. Soil Till Res 94:310–320

    Article  Google Scholar 

  • Qiu L, Wei X, Zhang X, Cheng J, Gale W, Guo C, Long T (2012) Soil organic carbon losses due to land use change in a semiarid grassland. Plant Soil 355:299–309

    Article  Google Scholar 

  • Raiesi F (2007) The conversion of overgrazed pastures to almond orchards and alfalfa cropping systems may favor microbial indicators of soil quality in Central Iran. Agric Ecosyst Environ 121:309–318

    Article  Google Scholar 

  • Raiesi F (2012) Soil properties and C dynamics in abandoned and cultivated farmlands in a semi-arid ecosystem. Plant Soil 351:161–175

    Article  Google Scholar 

  • Raiesi F, Beheshti A (2014) Soil specific enzyme activity shows more clearly soil responses to paddy rice cultivation than absolute enzyme activity in primary forests of northwest Iran. Appl Soil Ecol 75:63–70

    Article  Google Scholar 

  • Saggar S, Yeates GW, Shepherd TG (2001) Cultivation effects on soil biological properties, microfauna and organic matter dynamics in Eutric Gleysol and Gleyic Luvisol soils in New Zealand. Soil Till Res 58:55–68

    Article  Google Scholar 

  • Saviozzi RLM, Cardelli R, Riffaldi R (2001) A comparison of soil quality in adjacent cultivated, forest and native grassland soils. Plant Soil 233:251–259

    Article  Google Scholar 

  • Schlesinger WH, Andrews JA (2000) Soil respiration and the global carbon cycle. Biogeochemistry 48:7–20

    Article  Google Scholar 

  • Six J, Elliott ET, Paustian K, Doran JW (1998) Aggregation and soil organic matter accumulation in cultivated and native grassland soils. Soil Sci Soc Am J 62:1367–1377

    Article  Google Scholar 

  • Six J, Bossuyt H, Degryze S, Denef K (2004) A history of research on the link between (micro) aggregates, soil biota, and soil organic matter dynamics. Soil Till Res 79:7–31

    Article  Google Scholar 

  • Smith P (2008) Land use change and soil organic carbon dynamics. Nutr Cycl Agroecosyst 81:169–178

    Article  Google Scholar 

  • Soil Survey Staff (2010) Keys to soil taxonomy, 11th edn. USDA Natural Resources Conservation Service, Washington

    Google Scholar 

  • Sotomayor-Ramírez D, Espinoza Y, Acosta-Martínez V (2009) Land use effects on microbial biomass C, β-glucosidase and β-glucosaminidase activities, and availability, storage, and age of organic C in soil. Biol Fertil Soils 45:487–497

    Article  Google Scholar 

  • Sparling GP (1997) Soil microbial biomass, activity and nutrient cycling as indicators of soil health. In: Pankhurst CE, Doube BM, Gupta VVSR (eds) Biological indicators of soil health. CAB International, Wallingford, pp 97–119

    Google Scholar 

  • Sparling GP, Shepherd TG, Kettles HA (1992) Changes in soil organic C, microbial C and aggregate stability under continuous maize and cereal cropping, and after restoration to pasture in soils from the Manawatu region, New Zealand. Soil Till Res 24:225–241

    Article  Google Scholar 

  • Su YZ, Zhao HL, Zhang TH, Zhao XY (2004) Soil properties following cultivation and non-grazing of a semi-arid sandy grassland in northern China. Soil Till Res 75:27–36

    Article  Google Scholar 

  • Trasar-Cepeda C, Leiros MC, Gil-Stores F (2008) Hydrolytic enzyme activities in agricultural and forest soils. Some implications for their use as indicators of soil quality. Soil Biol Biochem 40:2146–2155

    Article  Google Scholar 

  • Urioste AM, Hevia GG, Hepper EN, Anton LE, Bono AA, Buschiazzo DE (2006) Cultivation effects on the distribution of organic carbon, total nitrogen and phosphorus in soils of the semiarid region of Argentinian Pampas. Geoderma 136:621–630

    Article  Google Scholar 

  • Waldrop MP, Balser TC, Firestone MK (2000) Linking microbial community composition to function in a tropical soil. Soil Biol Biochem 32:1837–1846

    Article  Google Scholar 

  • Wang WJ, Dalal RC, Moody PW, Smith CJ (2003) Relationships of soil respiration to microbial biomass, substrate availability and clay content. Soil Biol Biochem 35:273–284

    Article  Google Scholar 

  • Wang ZP, Han XG, Li LH (2008) Effects of grasslands conversion to croplands on soil organic carbon in the temperate Inner Mongolia. J Environ Manag 86:529–534

    Article  Google Scholar 

  • Wang B, Xue S, Liu GB, Zhang GH, Li G, Ren ZP (2012) Changes in soil nutrient and enzyme activities under different vegetations in the Loess Plateau area, Northwest China. Catena 92:186–195

    Article  Google Scholar 

  • Zhang J, Song C, Yang W (2007) Effects of cultivation on soil microbiological properties in a freshwater marsh soil in Northeast China. Soil Till Res 93:231–235

    Article  Google Scholar 

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Acknowledgments

Shahrekord University provided the financial support for the reported study. The constructive and critical comments provided by two anonymous reviewers are very much appreciated.

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  1. Department of Soil Sciences and Engineering, Faculty of Agriculture, Shahrekord University, P.O.Box 115, Shahrekord, Iran

    Fayez Raiesi & Ali Beheshti

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  1. Fayez Raiesi
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Raiesi, F., Beheshti, A. Soil C turnover, microbial biomass and respiration, and enzymatic activities following rangeland conversion to wheat–alfalfa cropping in a semi-arid climate. Environ Earth Sci 72, 5073–5088 (2014). https://doi.org/10.1007/s12665-014-3376-5

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