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Bioconversion of Sugarcane Bagasse into Value-Added Products by Bioaugmentation of Endogenous Cellulolytic and Fermentative Communities

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Abstract

The goals of this study were to describe a comprehensive taxonomic profile of bacterial communities endogenous from sugarcane bagasse (SCB) and from a thermophilic sludge formed mainly by Proteobacteria Actinobacteria and Firmicutes phylum and its potential as a bioaugmented inoculum for degradation of lignocellulosic biomass. Batch assays were performed using SCB as substrate at different condition: (RC) 2 g L−1 glucose, (R1i) 2 g L−1 unpretreated SCB, (R2i) 2 g L−1 hydrothermally pretreated SCB (at 210 °C for 15 min), (R3i) 2 g L−1 hydrothermally pretreated SCB (at 210 °C for 15 min) followed by alkaline delignification (NaOH—1 M), (R4i) 1 g L−1 unpretreated SCB plus 1 g L−1 hydrothermally pretreated SCB (at 210 °C for 15 min) followed by alkaline delignification. Hydrogen, methane and organic acids were the main metabolites produced during the fermentation. Maximum hydrogen (2.01 and 1.09 mol H2 mol−1 consumed soluble carbohydrates) were obtained in R1i and R2i, respectively. The highest organic acid (1051 mg L−1) and methane (0.92 mmo L−1) production were obtained in R4i.

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Acknowledgements

Thanks the financial support of the Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP- Process Number 2013/22346-6 and 2009/15984-0).

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  1. University of São Paulo, João Dagnone Avenue, 1100, 13563-120, São Carlos, SP, Brazil

    Laís Américo Soares, Juliana Kawanish Braga, Fabrício Motteran, Isabel Kimiko Sakamoto & Maria Bernadete Amâncio Varesche

  2. USP University of São Paulo, Trabalhador Sancarlense Avenue, 400, 13566-590, São Carlos, SP, Brazil

    Patrícia Aparecida Santiago Monteiro & Paulo Seleghim Jr.

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  1. Laís Américo Soares
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Soares, L.A., Braga, J.K., Motteran, F. et al. Bioconversion of Sugarcane Bagasse into Value-Added Products by Bioaugmentation of Endogenous Cellulolytic and Fermentative Communities. Waste Biomass Valor 10, 1899–1912 (2019). https://doi.org/10.1007/s12649-018-0201-5

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