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Influence of Protein Extraction Techniques of Different De-oiled Residues from Jatropha curcas L. on Protein Recovery and Techno-functional Properties

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Abstract

As Jatropha curcas L. is a non-edible energy plant biodiesel production from J. curcas L. crude oil is an important field of interest in order to avoid the ‘tank or table’ discussion. As a by-product from oil extraction, high amounts of Jatropha meals are obtained requiring a concept for its sustainable utilization. Due to the high protein content (up to 40 %) in Jatropha seed cakes, added value can be generated by extraction of these proteins for further applications. The present study compared an aqueous and an enzyme-assisted process for protein extraction from screw-pressed and aqueous de-oiled Jatropha residue. Additionally, different methods for protein recovery were evaluated. Aqueous-extracted proteins were recovered by ultrafiltration, isoelectric precipitation and/or lyophilisation while the proteins from enzyme-assisted extraction were only lyophilized. Functionality of the obtained protein products was determined indicating good emulsifying properties as well as a good gelation behaviour for the aqueous-extracted proteins. In general, proteins from enzyme-assisted extraction had slightly lower functionality. The study indicates potential for the application of Jatropha proteins as emulsifiers in biodegradable bags or as antifoam agents.

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Acknowledgments

This study was financially supported by the German Federal Ministry of Education and Research (BMBF). The authors are grateful to GEA Westfalia Separator AG (Germany) for supplying ADJR and to ASA Spezialenzyme GmbH (Germany) for supplying Protease A01 preparation. We thank Mrs. Sigrid Gruppe, Mrs. Evi Müller, Mrs. Elfriede Bischof and Mrs. Sigrid Bergmann for the chemical analysis.

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  1. Fraunhofer Institute for Process Engineering and Packaging (IVV), Freising, Germany

    G. Gofferjé, A. Zöttl, A. Stäbler, T. Herfellner & U. Schweiggert-Weisz

  2. Institute for Food Technology and Food Chemistry, Technical University Berlin, Berlin, Germany

    E. Flöter

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  1. G. Gofferjé
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Gofferjé, G., Zöttl, A., Stäbler, A. et al. Influence of Protein Extraction Techniques of Different De-oiled Residues from Jatropha curcas L. on Protein Recovery and Techno-functional Properties. Waste Biomass Valor 6, 225–235 (2015). https://doi.org/10.1007/s12649-014-9342-3

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