Abstract
The objective of this study was to evaluate the in vitro ovicidal activity, phytochemistry, and toxicity of a saline extract obtained from peel of Punica granatum L fruits. The ovicidal activity was evaluated by the hatching inhibition of eggs recovered from fecal samples of naturally infected goats; the phytochemical analysis was carried out using the fruit peel; and the toxicity was tested on Artemia salina, using saline extract. The results showed that the ovicidal effect of the tested extract was 99% (25 mg mL−1), 99% (12.5 mg mL−1), 98% (6.25 mg mL−1), and 95% (3.12 mg mL−1), higher than that of the control drug, thiabendazole (83%). The phytochemical analysis showed presence of phenols, anthraquinones, and condensed and hydrolysable tannins in the fruit extract. The toxicity test of the extract of P. granatum showed an LC50 of 6.19 mg mL−1, which indicates a safe use for a concentration of 3.12 mg mL−1, since it was the tested concentration that was below the reliable LC50. The saline extract from peels of P. granatum has ovicidal activity, important secondary metabolites, and absence of toxicity at the lowest concentration tested. However, in vivo tests in experimental models are recommended before performing experiments in ruminants.
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Our gratitude to financial support of Secretaria Nacional de Mobilidade e Desenvolvimento Regional e Urbano for intermediate of Ministério do Desenvolvimento Regional.
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RCSF and ACDSB conceived and designed the research. RCSF, TNB, CKOR, and JILB conducted the experiments. MDCS and KMPS contributed with new reagents and analytical tools. HPM and JCSP analyzed the data. RCSF and ACDSB wrote the manuscript. All authors read and approved the manuscript.
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da Silva Felix, R.C., Barbosa, T.N., Marques, H.P. et al. In vitro nematocidal activity of Punica granatum L. against gastrointestinal helminths in goats. J Parasit Dis 46, 236–242 (2022). https://doi.org/10.1007/s12639-021-01439-1
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DOI: https://doi.org/10.1007/s12639-021-01439-1