Skip to main content

Advertisement

SpringerLink
Log in

The impact of anthelminthic therapeutics on serological and tissues apoptotic changes induced by experimental trichinosis

  • Original Article
  • Published:
Journal of Parasitic Diseases Aims and scope Submit manuscript

Abstract

Trichinosis is a sharable parasitic disease caused by Trichinella spp., the disease occurred on eating inappropriate cooked pork infected by the parasite encysted larvae. This study aimed to evaluate experimentally the impact of treatment by thiabendazole, praziquantel (PZQ) and prednisone on T. spiralis induced parasitological, serological and apoptotic changes. Forty albino rats were infected orally each by ± 1000 larvae, divided into four groups each of 10 rats, group (A) infected control, group (B) thiabendazole tested, group (C) PZQ tested and group (D) prednisone tested. On the seventh and 40th days post-infection, all groups were evaluated parasitologically by the number of the intestinal worms and the muscular encysted larvae, while IFN-γ and TNF-α were estimated by ELISA, histopathological and histochemical assessment of the tissue changes during both phases were performed by different stains. In conclusion, thiabendazole was a potent and curable drug, it showed nearly 100% efficacy on intestinal worms, highly significant variations in cytokines levels during both the intestinal and muscular phases, while it induced moderate effects on encysted muscular larvae number, In addition it ameliorated myocytes apoptotic changes induced by trichinosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Andrews P, Thomas H, Ponlke R, Seubert J (1983) Praziquantel. Med Res Rev 3:143–200

    Article  Google Scholar 

  • Ashour DS, Elbakary RH (2011) Pathogenesis of restricted movements in trichinellosis: an experimental study. Exp Parasitol 128:414–418

    Article  PubMed  Google Scholar 

  • Beiting DP, Gagliardo LF, Hesse M, Bliss SK, Meskill D, Appleton JA (2007) Coordinated control of immunity to muscle stage Trichinella spiralis by IL-10, regulatory T cells, and TGF-beta. J Immunol 178:1039–1047

    Article  PubMed  CAS  Google Scholar 

  • Boonmars T, Wu Z, Nagano I, Takahashi Y (2004) Expression of apoptosis-related factors in muscles infected with Trichinella spiralis. Parasitology 128:323–332

    Article  PubMed  CAS  Google Scholar 

  • Boonmars T, Wu Z, Nagano I, Takahashi Y (2005) Trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than T. spiralis infection. Parasitol Res 97:13–20

    Article  PubMed  CAS  Google Scholar 

  • Boonmars T, Srirach P, Kaewsamut B, Srisawangwong T, Pinlaor S, Pinlaor P, Yongvanit P, Sithithaworn P (2008) Apoptosis-related gene expression in hamster opisthorchiasis post praziquantel treatment. Parasitol Res 102:447–455

    Article  PubMed  CAS  Google Scholar 

  • Bratton SB, Walker G, Srinivasula SM, Sun XM, Butterworth M, Alnemri ES, Cohen GM (2001) Recruitment, activation and retention of caspases-9 and -3 by Apaf-1 apoptosome and associated XIAP omplexes. EMBO J 20:998–1009

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Brow HD, Matzuk AR, Lives IR, Petrson LH, Harris SA, Sarett LM, Egerton JR, Yakstis JJ, Campbell WC, Cuckler AC (1961) Antiparasitic drugs :IV. (2-4-thia-zolil 1-Benzimidazole) a new anthelmintic. J Am Chem Soc 83:1764–1765

    Article  Google Scholar 

  • Campbell WC (1994) Meatborne helminth infections: trichinellosis. In: Hui SY, Murrell KD (eds) Food borne disease handbook. Marcel Dekker, New York, pp 255–277

    Google Scholar 

  • Campbell WC, Cuckler AC (1964) Effect of thiabendazole upon the enteral and parenteral phases of trichinosis in mice. J Parasitol 50:481–486

    Article  PubMed  CAS  Google Scholar 

  • Carleton MA, Drury GA, Willington EA, Cammeron H, Carleton S (1967) Histological technique, 4th edn. Oxford University Press, New York

    Google Scholar 

  • Castro DA, Malone C, Smith S (1980) Systemic anti-inflammatory effect associated with enteric trichinosis in the rat. J Parasitol 66:407–412

    Article  PubMed  CAS  Google Scholar 

  • Chieco P, Derenzini M (1999) The Feulgen reaction 75 years on. Histochem Cell Biol 111:345–358

    Article  PubMed  CAS  Google Scholar 

  • Dempsey PW, Doyle SE, He JQ, Cheng G (2003) The signaling adaptors and pathways activated by TNF superfamily. Cytokine Growth Factor Rev 14:193–209

    Article  PubMed  CAS  Google Scholar 

  • Denham DA (1965) Studies with methyridine and Trichinella spiralis. I. Effect upon the intestinal phase in mice. Exp Parasitol 17:10–14

    Article  PubMed  CAS  Google Scholar 

  • Despommier DD (2009) Trichinellida, Dioctophymatida and Enoplean Parasites, Ch. 23. In: Foundations of parasitology, 8th ed. McGraw-Hill companies, Inc., New York, pp 399–418

  • Dupouy-Camet J, Kociecka W, Bruschi F, Bolas-Fernandez F, Pozio E (2002) Opinion on the diagnosis and treatment of human trichinellosis. Expert Opin Pharmacother 3:1117–1130

    Article  PubMed  Google Scholar 

  • Dvorožňáková E, Hurníková Z, Kołodziej-Sobocińska M (2011) Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae. Parasitol Res 108:169–176

    Article  PubMed  Google Scholar 

  • El-Ridi AM, Abou-Ragab HA, Ismail MM, Shehata MM, Ramadan ME, Etewa SE (1990) Effect of some drugs on some histopathological and immunological aspects of experimental trichinosis in albino rats. J Egypt Soc Parasitol 20:99–104

    PubMed  CAS  Google Scholar 

  • Else KJ, Finkelman FD (1998) Intestinal nematode parasites, cytokines and effector mechanisms. Int J Parasitol 28:1145–1158

    Article  PubMed  CAS  Google Scholar 

  • Fabre MV, Beiting DP, Bliss SK, Appleton JA (2009) Immunity to Trichinella spiralis muscle infection. Vet Parasitol 159:245–248

    Article  PubMed  CAS  Google Scholar 

  • Frydas S, Papaioannou N, Hatzistilianou M, Merlitti D, Digioaacchino M, Castellanim L, Conti P (2002) Generation of TNFa, IFN-γ, IL-4, IL-6 and IL-10 in Trichinella spiralis infected mice: effect of the anti-inflammatory compound mimosine. Ammo Pharmacology 9(4):389–400

    CAS  Google Scholar 

  • Gamble HR, Bessonov AS, Cuperlovic K, Gajadhar AA, vanKnapen F, Noeckler K, Schenone H, Zhu X (2000) International Commission on trichinellosis: recommendations on methods for the control of Trichinella in domestic and wild animals intended for human consumption. Vet Parasitol 93:393–408

    Article  PubMed  CAS  Google Scholar 

  • García A, Barrera MG, Piccirilli G, Vasconi MD, Di Masso RJ, Leonardi D, Hinrichsen LI, Lamas MC (2013) Novel albendazole formulations given during the intestinal phase of Trichinella spiralis infection reduce effectively parasitic muscle burden in mice. Parasitol Int 62:568–570

    Article  PubMed  CAS  Google Scholar 

  • Gottstein B, Pozio E, Nöckler K (2009) Epidemiology, diagnosis, treatment, and control of trichinellosis. Clin Microbiol Rev 22:127–145

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Guenther S, Nöckler K, von Nickisch-Rosenegk M, Landgraf M, Ewers C, Wieler LH, Schierack P (2008) Detection of T. spiralis, T. britovi and T. pseudospiralis in muscle tissue with real-time PCR. J Micro-Boil Methods 75:287–292

    Article  CAS  Google Scholar 

  • Gupta S (2003) Molecular signaling in death receptor and mitochondrial pathways of apoptosis (Review). Int J Oncol 22:15–20

    PubMed  CAS  Google Scholar 

  • Harris DP, Haynes L, Sayles PC, Duso DK, Eaton SM, Lepak NM, Johnson LL, Swain SL, Lund FE (2000) Reciprocal regulation of polarized cytokine production by effector B and T cells. Nat Immunol 1:475–482

    Article  PubMed  CAS  Google Scholar 

  • Helmby H, Grencis RK (2003) IFN-γ independent effects of IL-12 during intestinal nematode infection. J Immunol 171:3691–3696

    Article  PubMed  CAS  Google Scholar 

  • Hoff J, Rlagt LV (2000) Methods of blood collection in the mouse. Lab Anim 29:47–53

    Google Scholar 

  • Hosking BC, Watson TG, Leathwick DM (1996) Multigeneric resistance to oxfendazole by nematodes in cattle. Vet Rec 138:67–68

    Article  PubMed  CAS  Google Scholar 

  • Jarczewdaska K, Gorny M, Zeromski J (1974) Immunological studies of rat skeletal muscles in the course of T. spiralis infection treated with thiabendazole proceedings, 3rd International conference on trichinellosis. Nov. 2–4, 1972. Miami Beach, Florid

  • Kisiel K, Kaszuba A (2011) Alclometasone dipropionate properties and clinical uses. Post Dermatol Alergol 2:107–119

    Google Scholar 

  • Kociecka W, Boczon K, Pozio E, van Knapen F (2004) Trichinella. In: Miliotis MD, Bier JW (eds) International handbook of foodborne pathogens. Marcel Dekker, New York, pp 37–658

    Google Scholar 

  • Lawerence C, Paterson J, Higgins L, MacDonald T, Kennedy M, Garside P (1998) IL-4-regulated enteropathy in an intestinal nematode infection. Eur J Immunol 28:2672–2684

    Article  Google Scholar 

  • Lee KM, Ko RC (2006) Cell-mediated response at the muscle phase of Trichinella pseudospiralis and Trichinella spiralis infections. Parasitol Res 99:70–77

    Article  PubMed  CAS  Google Scholar 

  • Leung LM, Barriaga LK (1966) Thiabendazole in the treatment of T. spiralis. Dt Med Wschr 60:70–72

    Google Scholar 

  • Lundy SK, Lerman SP, Boros DL (2001) Soluble egg antigen simulated T helper lymphocyte apoptosis and evidence for cell death mediated by FasL + T and B cells during murine Schistosoma mansoni infection. Infect Immun 1:271–280

    Article  Google Scholar 

  • Machnicka B, Katkiewicz M, Dziemian E (1994) Morphopathology of rats infected experimentally with Trichinella spiralis and treated with levamisole or dexamethasone. In: Campbell WC, Pozio E, Bruschi F (eds) Trichinellosis. Instituto Superiore di Sanita Press, Rome, pp 427–432

    Google Scholar 

  • Matsuo A, Wu Z, Nagano I, Takahashi Y (2000) Five types of nuclei present in the capsule of Trichinella spiralis. Parasitology 121:203–210

    Article  PubMed  Google Scholar 

  • Mikkonen TJ, Valkama H, Wihiman S, Sukura A (2005) Spatial variation of Trichinella prevalence in rats in finnish waste disposal sites. J Parasitol 91:210–213

    Article  PubMed  CAS  Google Scholar 

  • Osborne BA (1996) Apoptosis and the maintenance of homoeostasis in the immune system. Curr Opin Immunol 2:245–254

    Article  Google Scholar 

  • Piekarska J, Szczypka M, Michalski A, Obmińska-Mrukowicz B, Gorczykowski M (2010) The effect of immunomodulating drugs on the percentage of apoptotic and necrotic lymphocytes in inflammatory infiltrations in the muscle tissue of mice infected with Trichinella spiralis. Pol J Vet Sci 13:233–240

    PubMed  CAS  Google Scholar 

  • Pozio E (2007) World distribution of Trichinella spp. infections in animals and humans. Vet Parasitol 149:3–21

    Article  PubMed  Google Scholar 

  • Pozio E, Rinaldi L, Marucci G, Musella V, Galati F, Cringoli G, Boireau P, La Rosa G (2009) Hosts and habitats of Trichinella spiralis and Trichinella britovi in Europe. Int J Parasitol 39:71–79

    Article  PubMed  CAS  Google Scholar 

  • Schenone H (1980) Praziquantel in the treatment of Hymenolepis nana infections in children. J Trop Med Hyg 29(320):321

    Google Scholar 

  • Tato P, Fernández AM, Solano S, Borgonio V, Garrido E, Sepúlveda J, Molinari JL (2004) Acysteine protease from Taenia solium metacestodes induces apoptosis in human CD4+ T-cells. Parasitol Res 92:197–204

    Article  PubMed  CAS  Google Scholar 

  • Wajant H, Pfizenmaier K, Scheurich P (2003) Tumor necrosis factor signaling. Cell Death Differ 10:45–65

    Article  PubMed  CAS  Google Scholar 

  • Wu Z, Nagano I, Boonmars T, Takahashi Y (2005a) Tumor necrosis Factor receptor-mediated apoptosis in Trichinella spiralis infected muscle cells. Parasitology 131(Pt 3):373–381

    Article  PubMed  CAS  Google Scholar 

  • Wu Z, Nagano I, Boonmars T, Takahashi Y (2005b) A spectrum of functional genes mobilized after Trichinella spiralis infection in skeletal muscle. Parasitology 130(5):561–573

    Article  PubMed  CAS  Google Scholar 

  • Wu Z, Lj Sofronic-Milosavljevic, Nagano I, Takahashi Y (2008) Trichinella spiralis: nurse cell formation with emphasis on analogy to mus-cle cell repair. Parasit Vectors 1:27

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Samia E. Etewa, Ghada M. Fathy, Sara A. Abdel-Rahman, Dalia Abd El-Khalik, Mohamed H. Sarhan & Maha S. Badawey

Authors
  1. Samia E. Etewa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ghada M. Fathy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sara A. Abdel-Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dalia Abd El-Khalik
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohamed H. Sarhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Maha S. Badawey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sara A. Abdel-Rahman.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Etewa, S.E., Fathy, G.M., Abdel-Rahman, S.A. et al. The impact of anthelminthic therapeutics on serological and tissues apoptotic changes induced by experimental trichinosis. J Parasit Dis 42, 232–242 (2018). https://doi.org/10.1007/s12639-018-0990-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12639-018-0990-2

Keywords

Search

Navigation