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Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae

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Abstract

The murine cellular immune response to the infection with ten larvae of encapsulating (Trichinella spiralis, Trichinella britovi) and non-encapsulating species (Trichinella pseudospiralis) was studied. Both T. spiralis and T. britovi stimulated the proliferation of splenic T and B lymphocytes during the intestinal phase of infection, but T. spiralis activated the proliferative response also at the muscle phase, particularly in B cells. Non-encapsulating T. pseudospiralis stimulated the proliferation of T and B cells only on day 10 post-infection (p.i.) and later at the muscle phase. The numbers of splenic CD4 and CD8 T cells of T. spiralis infected mice were significantly increased till day 10 p.i., i.e., at the intestinal phase, and then at the late muscle phase, on day 60 p.i. T. britovi infection increased the CD4 and CD8 T cell numbers only on day 30 p.i. Decreased numbers of CD4 and CD8 T cells after T. pseudospiralis infection suggest a suppression of cellular immunity. Both encapsulating Trichinella species induced the Th2 response (cytokines interleukin-5 (IL-5) and interleukin-10) at the intestinal phase and the Th2 dominant response at the advanced muscle phase. Interferon-γ (IFN-γ) production (Th1 type) started to increase with migrating newborn larvae from day 15 p.i. till the end of the experiment. IL-5 production was suppressed during the intestinal phase of T. pseudospiralis infection. The immune response to T. pseudospiralis was directed more to the Th1 response at the muscle phase, the high IFN-γ production was found on day 10 p.i. and it peaked on days 45 and 60 p.i.

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Acknowledgements

This study was supported by the Slovak VEGA agency, grant no. 2/0071/08. The experimental protocols complied with the current Slovak ethic law.

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Authors and Affiliations

  1. Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

    Emília Dvorožňáková & Zuzana Hurníková

  2. Institute of Parasitology of the Polish Academy of Sciences, Twarda str 51/55, 00 818, Warsaw, Poland

    Marta Kołodziej-Sobocińska

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  1. Emília Dvorožňáková
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  2. Zuzana Hurníková
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  3. Marta Kołodziej-Sobocińska
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Correspondence to Emília Dvorožňáková.

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Dvorožňáková, E., Hurníková, Z. & Kołodziej-Sobocińska, M. Development of cellular immune response of mice to infection with low doses of Trichinella spiralis, Trichinella britovi and Trichinella pseudospiralis larvae. Parasitol Res 108, 169–176 (2011). https://doi.org/10.1007/s00436-010-2049-x

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