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The associations of C-reactive protein with serum levels of polyunsaturated fatty acids and trans fatty acids among middle-aged men from three populations

  • Published:
The journal of nutrition, health & aging

Abstract

Background

C-reactive protein (CRP) and many fatty acids (FAs) have been linked to cardiovascular disease. Associations of serum CRP with FAs in different populations have not been established.

Methods

Participants were 926 men aged 40-49 (2002-2006) from a population-based sample; 310 Whites from Pennsylvania, U.S., 313 Japanese from Shiga, Japan, and 303 Japanese Americans from Hawaii, U.S. Serum CRP (mg/L) was measured using immunosorbent assay while serum FAs (%) were measured using capillary-gasliquid chromatography.

Results

Whites had CRP (mg/L) levels higher than Japanese with Japanese Americans in-between (age-adjusted geometric mean “GM” 0.96, 0.38, 0.66, respectively). Whites had also higher levels of total n-6 FAs (%) and trans fatty acids (TFAs) but lower levels of marine-derived n-3 FAs compared to Japanese (41.78 vs. 35.05, 1.04 vs. 0.58, & 3.85 vs. 9.29, respectively). Japanese Americans had FAs levels in-between the other two populations. Whites had significant inverse trends between CRP and tertiles of total n-6 FAs (GM 1.20, 0.91 & 0.80; p=0.002) and marine-derived n-3 FAs (GM 1.22, 1.00 & 0.72; p<0.001) but a significant positive trend with TFAs (GM 0.80, 0.95 & 1.15; p=0.007). Japanese had a significant inverse trend between CRP and only total n-6 FAs (GM 0.50, 0.35 & 0.31; p<0.001). Japanese Americans had CRP associations with n-3 FAs, n-6 FAs, and TFAs similar to but weaker than Whites.

Conclusions

With the exception of consistent inverse association of CRP with total n-6 FAs, there are considerable variations across the three populations in the associations of CRP with different FAs.

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Abbreviations

CRP:

C-reactive protein

n-3 FAs:

n-3 fatty acids

n-6 FAs:

n-6 fatty acids

TFAs:

trans fatty acids

LDL cholesterol:

Low-density-lipoprotein cholesterol

HDL cholesterol:

High-density-lipoprotein cholesterol

References

  1. Ridker PM. Clinical application of C-reactive protein for cardiovascular disease detection and prevention. Circulation 2003; 107: 363–9.

    Article  PubMed  Google Scholar 

  2. Madsen T, Skou HA, Hansen VE, Fog L, Christensen JH, Toft E, et al. C-reactive protein, dietary n-3 fatty acids, and the extent of coronary artery disease. Am J Cardiol 2001; 88: 1139–42.

    Article  PubMed  CAS  Google Scholar 

  3. Micallef MA, Munro IA, Garg ML. An inverse relationship between plasma n-3 fatty acids and C-reactive protein in healthy individuals. Eur J Clin Nutr 2009; 63: 1154–6.

    Article  PubMed  CAS  Google Scholar 

  4. Niu K, Hozawa A, Kuriyama S, Ohmori-Matsuda K, Shimazu T, Nakaya N, et al. Dietary long-chain n-3 fatty acids of marine origin and serum C-reactive protein concentrations are associated in a population with a diet rich in marine products. Am J Clin Nutr 2006; 84: 223–9.

    PubMed  CAS  Google Scholar 

  5. Ohsawa M, Itai K, Onoda T, Tanno K, Sasaki S, Nakamura M, et al. Dietary intake of n-3 polyunsaturated fatty acids is inversely associated with CRP levels, especially among male smokers. Atherosclerosis 2008; 201: 184–91.

    Article  PubMed  CAS  Google Scholar 

  6. Masson S, Marchioli R, Mozaffarian D, Bernasconi R, Milani V, Dragani L, et al. Plasma n-3 polyunsaturated fatty acids in chronic heart failure in the GISSI-Heart Failure Trial: relation with fish intake, circulating biomarkers, and mortality. Am Heart J 2013; 165: 208–15 e4.

    Article  PubMed  CAS  Google Scholar 

  7. Balk EM, Lichtenstein AH, Chung M, Kupelnick B, Chew P, Lau J. Effects of omega-3 fatty acids on serum markers of cardiovascular disease risk: a systematic review. Atherosclerosis 2006; 189: 19–30.

    Article  PubMed  CAS  Google Scholar 

  8. Basu A, Devaraj S, Jialal I. Dietary factors that promote or retard inflammation. Arterioscler Thromb Vasc Biol 2006; 26: 995–1001.

    Article  PubMed  CAS  Google Scholar 

  9. Yoneyama S, Miura K, Sasaki S, Yoshita K, Morikawa Y, Ishizaki M, et al. Dietary intake of fatty acids and serum C-reactive protein in Japanese. J Epidemiol 2007; 17: 86–92.

    Article  PubMed  Google Scholar 

  10. Schmitz G, Ecker J. The opposing effects of n-3 and n-6 fatty acids. Prog Lipid Res 2008; 47: 147–55.

    Article  PubMed  CAS  Google Scholar 

  11. Mozaffarian D, Katan MB, Ascherio A, Stampfer MJ, Willett WC. Trans fatty acids and cardiovascular disease. N Engl J Med 2006; 354: 1601–13.

    Article  PubMed  CAS  Google Scholar 

  12. Okayama A, Ueshima H, Marmot M, Elliott P, Choudhury SR, Kita Y. Generational and regional differences in trends of mortality from ischemic heart disease in Japan from 1969 to 1992. Am J Epidemiol 2001; 153: 1191–8.

    Article  PubMed  CAS  Google Scholar 

  13. Ueshima H, Sekikawa A, Miura K, Turin TC, Takashima N, Kita Y, et al. Cardiovascular disease and risk factors in Asia: a selected review. Circulation 2008; 118: 2702–9.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Zhou BF, Stamler J, Dennis B, Moag-Stahlberg A, Okuda N, Robertson C, et al. Nutrient intakes of middle-aged men and women in China, Japan, United Kingdom, and United States in the late 1990s: the INTERMAP study. J Hum Hypertens 2003; 17: 623–30.

    Article  PubMed  CAS  Google Scholar 

  15. Stamler J, Elliott P, Chan Q. for the INTERMAP Research Group. INTERMAP Appendix Tables. Journal of Human Hypertension 2003; 17: 665–775.

    Article  Google Scholar 

  16. Sekikawa A, Ueshima H, Kadowaki T, El-Saed A, Okamura T, Takamiya T, et al. Less subclinical atherosclerosis in men in the post World-War-II birth cohort in Japan than in the United States Am J Epidemiol 2007; 165: 617–24.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Yano K, Reed DM, Kagan A. Coronary heart disease, hypertension and stroke among Japanese-American men in Hawaii: the Honolulu Heart Program. Hawaii Med J 1985; 44: 297–300, 12.

    PubMed  CAS  Google Scholar 

  18. Sekikawa A, Curb JD, Ueshima H, El-Saed A, Kadowaki T, Abbott RD, et al. Marine-derived n-3 fatty acids and atherosclerosis in Japanese, Japanese-American, and white men: a cross-sectional study. J Am Coll Cardiol 2008; 52: 417–24.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  19. Harris WS, Poston WC, Haddock CK. Tissue n-3 and n-6 fatty acids and risk for coronary heart disease events. Atherosclerosis 2007; 193: 1–10.

    Article  PubMed  CAS  Google Scholar 

  20. Ferrucci L, Cherubini A, Bandinelli S, Bartali B, Corsi A, Lauretani F, et al. Relationship of plasma polyunsaturated fatty acids to circulating inflammatory markers. J Clin Endocrinol Metab 2006; 91: 439–46.

    Article  PubMed  CAS  Google Scholar 

  21. Enzenbach C, Kroger J, Zietemann V, Jansen EH, Fritsche A, Doring F, et al. Erythrocyte membrane phospholipid polyunsaturated fatty acids are related to plasma C-reactive protein and adiponectin in middle-aged German women and men. Eur J Nutr 2011; 50: 625–36.

    Article  PubMed  CAS  Google Scholar 

  22. Poudel-Tandukar K, Nanri A, Matsushita Y, Sasaki S, Ohta M, Sato M, et al. Dietary intakes of alpha-linolenic and linoleic acids are inversely associated with serum C-reactive protein levels among Japanese men. Nutr Res 2009; 29: 363–70.

    Article  PubMed  CAS  Google Scholar 

  23. Steffen BT, Steffen LM, Tracy R, Siscovick D, Jacobs D, Liu K, et al. Ethnicity, plasma phospholipid fatty acid composition and inflammatory/endothelial activation biomarkers in the Multi-Ethnic Study of Atherosclerosis (MESA). Eur J Clin Nutr 2012; 66: 600–5.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  24. Calder PC. n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr 2006; 83: 1505S–19S.

    PubMed  CAS  Google Scholar 

  25. Takayama M, Arai Y, Sasaki S, Hashimoto M, Shimizu K, Abe Y, et al. Association of marine-origin N-3 polyunsaturated fatty acids consumption and functional mobility in the community-dwelling oldest old. J Nutr Health Aging 2013; 17: 82–89.

    Article  PubMed  CAS  Google Scholar 

  26. Arima H, Kubo M, Yonemoto K, Doi Y, Ninomiya T, Tanizaki Y, et al. Highsensitivity C-reactive protein and coronary heart disease in a general population of Japanese: the Hisayama study. Arterioscler Thromb Vasc Biol 2008; 28: 1385–91.

    Article  PubMed  CAS  Google Scholar 

  27. Wakugawa Y, Kiyohara Y, Tanizaki Y, Kubo M, Ninomiya T, Hata J, et al. C-reactive protein and risk of first-ever ischemic and hemorrhagic stroke in a general Japanese population: the Hisayama Study. Stroke 2006; 37: 27–32.

    Article  PubMed  CAS  Google Scholar 

  28. Pawlosky RJ, Hibbeln JR, Novotny JA, Salem N,. Physiological compartmental analysis of alpha-linolenic acid metabolism in adult humans. J Lipid Res 2001; 42: 1257–65.

    PubMed  CAS  Google Scholar 

  29. Wang C, Harris WS, Chung M, Lichtenstein AH, Balk EM, Kupelnick B, et al. n-3 Fatty acids from fish or fish-oil supplements, but not alpha-linolenic acid, benefit cardiovascular disease outcomes in primary-and secondary-prevention studies: a systematic review. Am J Clin Nutr 2006; 84: 5–17.

    PubMed  CAS  Google Scholar 

  30. Willett WC. Trans fatty acids and cardiovascular disease—epidemiological data. Atherosclerosis Supplements 2006; 7: 5–8.

    Article  PubMed  CAS  Google Scholar 

  31. Mozaffarian D. Trans fatty acids-Effects on systemic inflammation and endothelial function. Atherosclerosis Supplements 2006; 7: 29–32.

    Article  PubMed  CAS  Google Scholar 

  32. Ma J, Folsom AR, Shahar E, Eckfeldt JH. Plasma fatty acid composition as an indicator of habitual dietary fat intake in middle-aged adults. The Atherosclerosis Risk in Communities (ARIC) Study Investigators., 1995; 564–71.

    Google Scholar 

  33. Kobayashi M, Sasaki S, Kawabata T, Hasegawa K, Akabane M, Tsugane S. Single measurement of serum phospholipid fatty acid as a biomarker of specific fatty acid intake in middle-aged Japanese men. Eur J Clin Nutr 2001; 55: 643–50.

    Article  PubMed  CAS  Google Scholar 

  34. Ueshima H, Okayama A, Saitoh S, Nakagawa H, Rodriguez B, Sakata K, et al. Differences in cardiovascular disease risk factors between Japanese in Japan and Japanese-Americans in Hawaii: the INTERLIPID study. J Hum Hypertens 2003; 17: 631–9.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Graduate School of Public Health, University of Pittsburgh, Pittsburgh, USA

    A. El-Saed, R.W. Evans, S. Lee, L.H. Kuller & Akira Sekikawa

  2. Community Medicine Department, Faculty of Medicine, Mansoura University, Cairo, Egypt

    A. El-Saed

  3. University of Hawaii, Honolulu, Hawaii, USA

    K. Masaki & B.J. Willcox

  4. Shiga University of Medical Science, Shiga, Japan

    T. Okamura, H. Maegawa, A. Fujiyoshi, K. Miura & H. Ueshima

  5. Kyoto Women’s University, Kyoto, Japan

    Y. Nakamura

  6. Queen’s Hospital, Honolulu, Hawaii, USA

    T.B. Seto

  7. University of Korea, Seoul, Korea

    J. Choo

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  1. A. El-Saed
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  2. K. Masaki
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  3. T. Okamura
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  4. R.W. Evans
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  7. S. Lee
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  14. H. Ueshima
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El-Saed, A., Masaki, K., Okamura, T. et al. The associations of C-reactive protein with serum levels of polyunsaturated fatty acids and trans fatty acids among middle-aged men from three populations. J Nutr Health Aging 20, 16–21 (2016). https://doi.org/10.1007/s12603-016-0670-9

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  • DOI: https://doi.org/10.1007/s12603-016-0670-9

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