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Microencapsulation of riboflavin-producing Lactiplantibacillus Plantarum MTCC 25,432 and Evaluation of its Survival in Simulated Gastric and Intestinal Fluid

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Abstract

Microencapsulation is an optimistic method for the delivery of live microbial cells through different food products. In this study, riboflavin-producing probiotic strain Lactiplantibacillus plantarum MTCC 25,432 was encapsulated using a spray drying technique with different wall materials including Inulin, maltodextrin (MD), and MD + Inulin (1:1). The obtained spray dried powder was investigated for probiotic viability, encapsulation efficiency, particle size, water activity, moisture content, hygroscopicity, bulk and tapped densities, storage stabilities, Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). Besides this, the viability of the free and encapsulated probiotic cells was tested under simulated gastric and intestinal fluid conditions. In the results, microcapsules produced with the combination of MD + Inulin showed higher dry powder yield (36.5%) and viability of L. plantarum MTCC 25,432 (7.4 log CFU / g) as compared with individual coating materials. Further characterization revealed that MD + Inulin microcapsules are spherical (3.50 ± 1.61 μm in diameter) in shape with concavities, showed the highest encapsulation efficiency (82%), low water activity (0.307), moisture content (3.67%) and good survival ability at low pH (pH 2.0 and 3.0), high bile salt concentrations (1.0% and 2.0%), and long storage conditions. No differences in FTIR spectra were observed among the tested samples. However, TGA showed enhanced thermal stability of probiotic-loaded microcapsules when MD + Inulin was used together. In conclusion, MD + Inulin could be a potential encapsulation material for riboflavin-producing probiotic bacteria L. plantarum MTCC 25,432.

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Funding

The work was funded by the National Institute of Food Technology Entrepreneurship and Management (NIFTEM), Sonepat, India.

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Authors and Affiliations

  1. Department of Basic and Applied Sciences, NIFTEM, Sonepat, Haryana, India

    Vikram Kumar, Amrutha R., Sahil Nain & Neetu Kumra Taneja

  2. Dr. Reddy’s Laboratories Limited, Hyderabad, India

    Jayesh J. Ahire

  3. Centre for Advanced Translational Research in Food Nanobiotechnology (CATR-FNB), NIFTEM, Sonepat, Haryana, India

    Neetu Kumra Taneja

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  1. Vikram Kumar
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Contributions

Taneja NK and Vikram contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Vikram, Amrutha and Sahil Nain. The first draft of the manuscript was written by Vikram. Ahire JJ edited the manuscript extensively and wrote the final draft. Taneja NK reviewed and approved the draft. All authors read and approved the final manuscript.

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Correspondence to Neetu Kumra Taneja.

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Competing interests

Ahire JJ was employed by Dr. Reddy’s Laboratories Limited. Dr. Reddy’s Laboratories had no direct and indirect role in the study design/analysis/writing of this article. Other authors have no conflict of interest to declare.

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Kumar, V., Ahire, J.J., R., A. et al. Microencapsulation of riboflavin-producing Lactiplantibacillus Plantarum MTCC 25,432 and Evaluation of its Survival in Simulated Gastric and Intestinal Fluid. Probiotics & Antimicro. Prot. (2023). https://doi.org/10.1007/s12602-023-10115-0

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