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Evaluation of the Potential Probiotic Yeast Characteristics with Anti-MRSA Abilities

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a disreputable pathogenic bacterium that has been proven to colonize the intestinal tract. The goal of this study is to find anti-MRSA probiotic yeast from food and evaluate its probiotic characteristics and safety. Finally, 15 strains were isolated from fruit peel with anti-MRSA ability. Using DNA sequence analysis, they were identified as the genus Hanseniaspora (7 strains) and Starmerella (8 strains). Starmerella bacillaris CC-PT4 (CGMCC No. 23573) that was isolated from the grape peel has good auto-aggregation ability and hydrophobicity, and can tolerate 0.3% bile, pH 2, simulated gastric fluid (SGF), and simulated intestinal fluid (SIF). Strikingly, Starmerella bacillaris CC-PT4, like commercial probiotic Saccharomyces boulardii CNCM I-745 (Florastor ®), can adapt to the temperature of the human body (37 ℃). After safety assessment, this strain is sensitive to amphotericin B and cannot produced β-hemolytic activities. Overall, this study provides a new candidate for probiotic yeast with anti-MRSA ability.

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Data Availability Statement

All data generated or analyzed during this study are included in this article.

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Funding

The work was supported by the National Natural Science Foundation of China (No. 31772082), and the 13th Five-Year Plan Science and Technology Project of the Education Department of Jilin Province (JJKH20201017KJ).

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  1. College of Food Science and Engineering, Jilin University, Changchun, 130062, People’s Republic of China

    Yong Shen, Xue Bai, Yan Zhang, Qian Gao, Xiujuan Bu, Ying Xu & Na Guo

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Shen, Y., Bai, X., Zhang, Y. et al. Evaluation of the Potential Probiotic Yeast Characteristics with Anti-MRSA Abilities. Probiotics & Antimicro. Prot. 14, 727–740 (2022). https://doi.org/10.1007/s12602-022-09942-4

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