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Anti-adipogenic Effects of the Probiotic Lactiplantibacillus plantarum KU15117 on 3T3-L1 Adipocytes

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Abstract

In this study, we investigated the probiotic properties and anti-obesity effects of bacterial strains isolated from homemade kimchi. Lactiplantibacillus plantarum KU15117 was isolated using lactobacilli selective medium. L. plantarum KU15117 did not produce β-glucuronidase and showed high tolerance to artificial gastric juice and bile salt, acceptable resistance to antibiotics, and high adhesion ability to HT-29 cells. The anti-adipogenic activity of L. plantarum KU15117 at 109 CFU/well was confirmed by the reduction of oil red O staining and intracellular triglyceride level. Additionally, the expression levels of fatty acid synthase, CCAAT/enhance-binding protein-α, and peroxisome proliferator-activated receptor-γ, which are associated with the early stage of adipocyte differentiation, were significantly lower in the probiotic-treated group than in the control group. These results suggest that L. plantarum KU15117 has probiotic properties and anti-obesity effects and could be used as a prophylactic probiotics.

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Data Availability Statement

All data generated or analyzed during this study are included in this article.

Abbreviations

FAS:

Fatty acid synthase

C/EBP-α:

CCAAT/enhance-binding protein-α

PPAR-γ:

Peroxisome proliferator-activated receptor-γ

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Authors and Affiliations

  1. Department of Food Science and Biotechnology of Animal Resource, Konkuk University, Seoul, 05029, Republic of Korea

    Kyoung Jun Han, Na-Kyoung Lee, Hyung-Seok Yu & Hyun-Dong Paik

  2. Department of Food Science, Sun Moon University, Asan, 31460, Republic of Korea

    Hoon Park

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  1. Kyoung Jun Han
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  2. Na-Kyoung Lee
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Contributions

Kyoung Jun Han: investigation, methodology, writing—original draft, validation. Na-Kyoung Lee: conceptualization, investigation, methodology, validation, writing—review and editing. Hyung-Seok Yu: investigation, methodology. Hoon Park: writing—review and editing, validation. Hyun-Dong Paik: conceptualization, supervision, writing—review and editing, validation.

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Correspondence to Hyun-Dong Paik.

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Han, K.J., Lee, NK., Yu, HS. et al. Anti-adipogenic Effects of the Probiotic Lactiplantibacillus plantarum KU15117 on 3T3-L1 Adipocytes. Probiotics & Antimicro. Prot. 14, 501–509 (2022). https://doi.org/10.1007/s12602-021-09818-z

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