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Stress-induced defense in sorghum in response to attack by the spotted stem borer, Chilo partellus (Swinhoe)

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Abstract

There is a complex interplay of biochemical interactions which determine the host plant reaction to herbivory. We studied the Chilo partellus-induced biochemical plant defense system in sorghum. Present studies found that the total protein, starch and total sugar contents were significantly lower, while antioxidant enzymes like ascorbate oxidase (AO), catalase (CAT), ascorbate peroxidase (APX), phenyl ammonia lyase (PAL), tyrosine ammonia lyase (TAL), and nonenzymatic antioxidants such as total phenols, total antioxidants and ferric ion reducing antioxidant power (FRAP) higher in the seedlings of all the test sorghum genotypes as compared to susceptible genotype, Swarna both under healthy and C. partellus damaged conditions. Further, the C. partellus damage resulted in significant increase in amounts of all the test constitutional, enzymatic and nonenzymatic biochemicals in the test genotypes, however their percent increase was highly variable across genotypes. These studies demonstrate that both constitutive and C. partellus damage induced enzymatic and nonenzymatic antioxidants like APX, AO, PAL, TAL, CAT, total phenols and FRAP were significantly greater in IS 2205, IS 18,551, ICSV 1, ICSV 700 and ICSV 93,046 than in susceptible check (Swarna) indicating their role in plant defense. Furthermore, these genotypes can be used in sorghum breeding for resistance against C. partellus.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The necessary facilities and senior research fellowship to the first author by ICAR-Indian Agricultural Research Institute, New Delhi are gratefully acknowledged.

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Authors and Affiliations

  1. Division of Entomology, ICAR-Indian Agricultural Research Institute, 110 012, New Delhi, India

    Hemant Kumar, Mukesh K. Dhillon & Tanmaya K. Bhoi

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  1. Hemant Kumar
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  2. Mukesh K. Dhillon
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  3. Tanmaya K. Bhoi
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Contributions

Mukesh K. Dhillon and Hemant Kumar conceptualized and designed the study. Experimental set up, data collection and analysis were performed by Hemant Kumar and Tanmaya K. Bhoi. The manuscript was written by Mukesh K. Dhillon and all authors read and approved the final manuscript.

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Correspondence to Mukesh K. Dhillon.

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Kumar, H., Dhillon, M.K. & Bhoi, T.K. Stress-induced defense in sorghum in response to attack by the spotted stem borer, Chilo partellus (Swinhoe). Phytoparasitica 51, 49–61 (2023). https://doi.org/10.1007/s12600-022-01037-8

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