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Cuscuta spp. populations as potential reservoirs and vectors of four plant viruses

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Abstract

Members of the genus Cuscuta or dodders represent widely distributed holoparasitic flowering plants with large agricultural and ecological significance. Besides their direct negative impact on the growth and development of host plants, they are also regarded as putative vectors of various pathogens, including plant viruses. In the present study, a total of 36 populations, belonging to 4 Cuscuta species from Bulgaria were tested for the presence of 4 of the most damaging and widely distributed plant viruses. It was found that while a significant proportion of the populations of Cuscuta campestris, which is introduced in the country were positive for Tomato Yellow Leaf Curl Virus (19%) and Cucumber Mosaic Virus (30%), only a single population of the other 3 species (native to the country) was positive for both. No occurrence of Tobacco Mosaic Virus or Alfalfa Mosaic Virus was detected. Furthermore, the C. campestris was also shown to be effective in transmitting viral infection to host plants. Overall, the presented results suggest that the introduced parasitic plant species is a significant reservoir and vector for viral diseases, which represents a further concern about its agricultural impact.

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References

  • Ali, A., Li, H., Schneider, W. L., Sherman, D. J., Gray, S., Smith, D., & Roossinck, M. J. (2006). Analysis of genetic bottlenecks during horizontal transmission of Cucumber mosaic virus. Journal of Virology, 80, 8345–8350.

    Article  CAS  Google Scholar 

  • Assyov, B., Petrova, A., Dimitrov, D., & Vassilev, R. (2012). Konspekt na vishata flora na Bulgaria Horologi I florin elementi [Conspectus of the Bulgarian vascular flora. Distribution maps and floristic elements] Sofia: Bulgarian Biodiversity Foundation, Sofia, Bulgaria

  • Baráth, K. (2021). Effect of species environment on host preference of Cuscuta campestris. Plant Ecology, 222, 1023–1032.

  • Barath, K., & Csiky, J. (2012). Host range and host choice of Cuscuta species in Hungary. Acta Botanica Croatica, 71, 215–227.

    Article  Google Scholar 

  • Birschwilks, M., Haupt, S., Hofius, D., & Neumann, S. (2006). Transfer of phloem-mobile substances from the host plants to the holoparasite Cuscuta sp. Journal of Experimental Botany, 57, 911–921.

    Article  CAS  Google Scholar 

  • Dikova, B. (2006) Establishment of tobacco rattle virus (TRV) in weeds and Cuscuta. Biotechnology & Biotechnological Equipment, 20, 42–48.

  • Dimitrova, T. (2004). Check of Amaranthus blitoides W. var. Reverchoni Th.-an element of the control of Cuscuta epithymum Murr in lucerne (Medicago sativa L.). Bulgarian Journal of Agricultural Science, 10, 579–582.

  • Dimitrova, T. (2011) Study about the Control of Cuscuta spp. in Alfalfa Medicago sativa L. Plant Science (Bulgaria), 48, 69-575.

  • Gal-On, A., et al. (2009). Broomrape can acquire viruses from its hosts. Phytopathology, 99, 1321–1329.

    Article  CAS  Google Scholar 

  • Gildow, F., Shah, D., Sackett, W., Butzler, T., Nault, B., & Fleischer, S. (2008). Transmission efficiency of Cucumber mosaic virus by aphids associated with virus epidemics in snap bean. Phytopathology, 98, 1233–1241.

    Article  CAS  Google Scholar 

  • Haupt, S., Oparka, K. J., Sauer, N., & Neumann, S. (2001). Macromolecular trafficking between Nicotiana tabacum and the holoparasite Cuscuta reflexa. Journal of Experimental Botany, 52, 173–177.

    Article  CAS  Google Scholar 

  • Hibberd, J. M., & Dieter Jeschke, W. (2001). Solute flux into parasitic plants. Journal of Experimental Botany, 52, 2043–2049.

    Article  CAS  Google Scholar 

  • Ho, A. (2017). Diversity and evolution of fruits in Cuscuta (dodders; Convolvulaceae), Thesis, Wilfrid Laurier University, Ontario, Canada

  • Hsiao, C., Jacobs, S., Chatterton, N., & Asay, K. (1998). A molecular phylogeny of the grass family (Poaceae) based on the sequences of nuclear ribosomal DNA (ITS). Australian Systematic Botany, 11, 667–688.

  • Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.

  • Li, M., Hu, J., Xu, F.-C., & Liu, S.-S. (2010). Transmission of Tomato Yellow Leaf Curl Virus by two invasive biotypes and a Chinese indigenous biotype of the whitefly Bemisia tabaci. International Journal of Pest Management, 56, 275–280.

    Article  CAS  Google Scholar 

  • Malapi-Nelson, M., Wen, R.-H., Ownley, B., & Hajimorad, M. (2009). Co-infection of soybean with Soybean mosaic virus and Alfalfa mosaic virus results in disease synergism and alteration in accumulation level of both viruses. Plant Disease, 93, 1259–1264.

    Article  CAS  Google Scholar 

  • McNeal, J.R., Arumugunathan, K., Kuehl, J.V., Boore, J.L., & dePamphilis, C. W. (2007). Systematics and plastid genome evolution of the cryptically photosynthetic parasitic plant genus Cuscuta (Convolvulaceae). BMC Biology, 5, 1–19.

  • Mikona, C., & Jelkmann, W. (2010). Replication of Grapevine leafroll-associated virus-7 (GLRaV-7) by Cuscuta species and its transmission to herbaceous plants. Plant Disease, 94, 471–476.

  • Parker, C. (2012) Parasitic weeds: a world challenge. Weed Science, 60, 269–276.

  • Petrova, A., Vladimírov-Hurban, V., & Georgiev, V. (2013). Invasive alien species of vascular plants in Bulgaria. Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria

  • Petrova, A., & Vladimirov, V. (2010). Balkan endemics in the Bulgarian flora Phytologia. Balcanica, 16, 293–311.

    Google Scholar 

  • Scholthof, K.B.G. et al. (2011). Top 10 plant viruses in molecular plant pathology. Molecular Plant Pathology, 12, 938–954.

  • Shen, H., Hong, L., Ye, W., Cao, H., & Wang, Z. (2007). The influence of the holoparasitic plant Cuscuta campestris on the growth and photosynthesis of its host Mikania micrantha. Journal of Experimental Botany, 58, 2929–2937.

    Article  CAS  Google Scholar 

  • Tamura, K., & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526.

    CAS  PubMed  Google Scholar 

  • Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences, 76, 4350–4354.

    Article  CAS  Google Scholar 

  • Varner, J., & Ye, Z. (1994). Tissue Printing. the FASEB Journal, 8, 378–284.

    Article  CAS  Google Scholar 

  • White, T.J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18, 315–322.

  • Xu, H., & Nie, J. (2006) Identification, characterization, and molecular detection of Alfalfa mosaic virus in potato. Phytopathology, 96, 1237–1242.

  • Zagorchev, L., Traianova, A., Teofanova, D., Li, J., Kouzmanova, M., & Goltsev, V. (2020). Influence of Cuscuta campestris Yunck. on the photosynthetic activity of Ipomoea tricolor Cav.-in vivo chlorophyll a fluorescence assessment. Photosynthetica, 58, 422–432.

  • Zhekova, E., Petkova, D., & Ivanova, I. (2014). Smicronyx smreczynskii F. Solari, 1952 (Insecta: Curculionidae): Possibilities for biological control of two Cuscuta species (Cuscutaceae) in district of Ruse. Acta Zoologica Bulgarica, 66, 431–432.

  • Zhuang, H. et al. (2018). Aphid (Myzus persicae) feeding on the parasitic plant dodder (Cuscuta australis) activates defense responses in both the parasite and soybean host. New Phytologist, 218, 1586–1596.

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Funding

This research was funded by the National Science Fund of Ministry of Education and Science, Bulgaria, grant number КП-06-H31/10.

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Authors and Affiliations

  1. Faculty of Biology, Sofia University, 8 Dragan Tsankov blvd., 1164, Sofia, Bulgaria

    Denitsa Teofanova, Yana Lozanova, Kosara Lambovska, Kalina Pachedjieva, Anita Tosheva, Mariela Odjakova & Lyuben Zagorchev

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  1. Denitsa Teofanova
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  2. Yana Lozanova
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  3. Kosara Lambovska
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  4. Kalina Pachedjieva
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  5. Anita Tosheva
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  6. Mariela Odjakova
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  7. Lyuben Zagorchev
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Contributions

LZ, MO, and DT designed the experiment and wrote the manuscript. KP and AT collected the material and performed morphological taxonomy. YL, KL, and DT performed the experiments and analyzed the data. All authors agreed on the final version of the manuscript.

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Correspondence to Lyuben Zagorchev.

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Teofanova, D., Lozanova, Y., Lambovska, K. et al. Cuscuta spp. populations as potential reservoirs and vectors of four plant viruses. Phytoparasitica 50, 555–566 (2022). https://doi.org/10.1007/s12600-022-00981-9

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  • DOI: https://doi.org/10.1007/s12600-022-00981-9

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