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Analysis of the morphological variation of Doryteuthis sanpaulensis (Cephalopoda: Loliginidae) in Argentinian and Brazilian coastal waters using geometric morphometrics techniques

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Abstract

Shape variation of individuals from three locations, two from Argentina and one from Brazil, was analysed by geometric morphometrics techniques. Individuals were weighed, measured, sexed, assigned to a maturity stage and photographed. For the analysis, two landmark configurations were used: one of nine landmarks (9L) and another of 25 landmarks (25L). To visualise shape variation, a principal component analysis (PCA) on Procrustes coordinates was conducted. The percentage of total shape variation explained by the 9L configuration was 80.5% (PC1: 61% and PC2: 19.5%), while the percentage for the 25L configuration was 76.4% (PC1: 54.8% and PC2: 21.6%). Based on this, shape analyses were performed using only the 9L configuration. The PCA results indicated that specimens show a large overlap between the categories based on sex and maturity stage. When location is considered, squid shape from São Sebastião and Rawson Port were very similar, while in Nuevo Gulf, the presence of two different morphological groups were observed: one similar to São Sebastião and Rawson Port, and the other with a morphology typical of Nuevo Gulf. Thus, two different morphotypes were defined: (i) morphotype of the continental shelf and (ii) morphotype typical of the gulf. The continental shelf morphotype is characterised by larger fins and wider mantle than the gulf morphotype. These results suggest that the morphotypes of Doryteuthis sanpaulensis are adaptations to oceanographic regimens.

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References

  • Adams DC, Rohlf FJ, Slice DE (2004) Geometric morphometrics: ten years of progress following the ‘revolution’. Ital J Zool 71(1):5–16

    Article  Google Scholar 

  • Barón PJ (2001a) First description and survey of the egg masses of Loligo gahi (d’Orbigny, 1835) and Loligo sanpaulensis (Brakoniecki, 1984) from coastal waters of Patagonia. J Shellfish Res 20(1):289–295

    Google Scholar 

  • Barón PJ (2001b) Evaluación de las aguas costeras nordpatagónicas, entre los 42°S y 44°S, como área reproductiva de calamares loligínidos. Dissertation, Universidad Nacional del Comahue

  • Barón PJ, Ré ME (2002a) Morphometry of the northern Patagonian sympatric populations of Loligo sanpaulensis and Loligo gahi. J Mar Biol Assoc U K 82(02):269–278

    Article  Google Scholar 

  • Barón PJ, Ré ME (2002b) Reproductive cycle and population structure of Loligo sanpaulensis of the northeastern coast of Patagonia. Bull Mar Sci 71(1):175–186

    Google Scholar 

  • Belcari P (1996) Length–weight relationships in relation to sexual maturation of Illex coindetii (Cephalopoda: Onunastrephidae) in the northern Tyrrhenian Sea (Western Mediterranean). Sci Mar 60(2–3):379–384

    Google Scholar 

  • Bookstein FL (1991) Morphometric tools for landmark data: geometry and biology. Cambridge University Press, Cambridge

    Google Scholar 

  • Bookstein FL (1997) Shape and the information in medical images: a decade of the morphometric synthesis. Comput Vis Image Underst 66:97–118

    Article  Google Scholar 

  • Bookstein FL (1998) A hundred years of morphometrics. Acta Zool Acad Sci Hung 44(1-2):7–59

    Google Scholar 

  • Boyle PR (1990) Cephalopod biology in the fisheries context. Fish Res 8(4):303–321

    Article  Google Scholar 

  • Boyle PR, Boletzky SV (1996) Cephalopod populations: definition and dynamics. Philos Trans R Soc Lond B Biol Sci 351(1343):985–1002

    Article  Google Scholar 

  • Boyle P, Rodhouse P (2005) From egg to recruitment. In: Boyle P, Rodhouse P (eds) Cephalopods: ecology and fisheries, 1st edn. Blackwell Publishing, Oxford

    Chapter  Google Scholar 

  • Brunetti NE, Ivanovic ML, Sakai M (1999) Calamares de importancia comercial en Argentina. Biología, distribución, pesquerías y muestreo biológico. Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Mar del Plata, 45 pp

    Google Scholar 

  • Cadrin SX (2000) Advances in morphometric identification of fishery stocks. Rev Fish Biol Fish 10(1):91–112

    Article  Google Scholar 

  • Castro Filho BM, Miranda LB, Miyao SY (1987) Condições hidrográficas na plataforma continental ao largo de Ubatuba: variações sazonais e em média escala. Bol Inst Oceanogr Sao Paulo 35(2):135–151

    Article  Google Scholar 

  • Costa PAS, Fernandes FC (1993) Reproductive cycle of Loligo sanpaulensis (Cephalopoda: Loliginidae) in the Cabo Frio region, Brazil. Mar Ecol Prog Ser 101(1–2):91–97

    Article  Google Scholar 

  • Crespi-Abril AC, Morsan EM, Barón PJ (2010) Analysis of the ontogenetic variation in body and beak shape of the Illex argentinus inner shelf spawning groups by geometric morphometrics. J Mar Biol Assoc U K 90(03):547–553

    Article  Google Scholar 

  • Crespi-Abril AC, Gomila GV, Venerus LA, Barón PJ (2014) Spatial distribution of cephalopod paralarvae in San José Gulf (Northern Patagonia, Argentina): the role of tidal circulation in larval dispersal. Fish Res 152:13–20

    Article  Google Scholar 

  • De Pierrepont JF, Dubois B, Desormonts S, Santos MB, Robin JP (2005) Stomach contents of English Channel cetaceans stranded on the coast of Normandy. J Mar Biol Assoc U K 85(06):1539–1546

    Article  Google Scholar 

  • dos Santos RA, Haimovici M (2002) Cephalopods in the trophic relations off southern Brazil. Bull Mar Sci 71(2):753–770

    Google Scholar 

  • Dellatorre FG, Pisoni JP, Barón PJ, Rivas AL (2012) Tide and wind forced nearshore dynamics in Nuevo Gulf (Northern Patagonia, Argentina): potential implications for cross-shore transport. J Mar Syst 96:82–89

    Article  Google Scholar 

  • Dryden IL, Mardia KV (1998) Statistical shape analysis. Wiley, New York

    Google Scholar 

  • Forsythe JW (1993) A working hypothesis on how seasonal temperature change may impact the field growth of young cephalopods. In: Okutani T, O’Dor RK, Kubodera T (eds) Recent advances in cephalopod fisheries biology. Tokai University Press, Tokyo, pp 133–144

    Google Scholar 

  • Forsythe JW, Walsh LS, Turk PE, Lee PG (2001) Impact of temperature on juvenile growth and age at first egg-laying of the Pacific reef squid Sepioteuthis lessoniana reared in captivity. Mar Biol 138(1):103–112

    Article  Google Scholar 

  • Garri R, Ré ME (2002) Morphology of the digestive apparatus of Enteroctopus megalocyathus and Loligo sanpaulensis (Mollusca, Cephalopoda). Iheringia Ser Zool 92(2):81–91

    Article  Google Scholar 

  • Hatfield EM (2000) Do some like it hot? Temperature as a possible determinant of variability in the growth of the Patagonian squid, Loligo gahi (Cephalopoda: Loliginidae). Fish Res 47(1):27–40

    Article  Google Scholar 

  • Hatfield EM, Hanlon RT, Forsythe JW, Grist EP (2001) Laboratory testing of a growth hypothesis for juvenile squid Loligo pealeii (Cephalopoda: Loliginidae). Can J Fish Aquat Sci 58(5):845–857

    Article  Google Scholar 

  • Juanicó M (1983) Multi-populational structure of Loligo brasiliensis, a neritic squid. In: Shuval HI (ed) Developments in ecology and environmental quality. Balaban International Science Services, Rehovot, pp 337–345

    Google Scholar 

  • Klingenberg CP (2011) MorphoJ: an integrated software package for geometric morphometrics. Mol Ecol Res 11:353–357

    Article  Google Scholar 

  • Laptikhovsky V, Remeslo A, Nigmatullin CM, Polishchuk IA (2001) Recruitment strength forecasting of the shortfin squid Illex argentinus (Cephalopoda: Ommastrephidae) using satellite SST data, and some consideration of the species’ population structure. Copenhagen Denmark ICES K 15:1–9

    Google Scholar 

  • Lima ID, Garcia CA, Möller OO (1996) Ocean surface processes on the southern Brazilian shelf: characterization and seasonal variability. Cont Shelf Res 16(10):1307–1317

    Article  Google Scholar 

  • Lipiński MR, Butterworth DS, Augustyn CJ, Brodziak JKT, Chrsty G, Des Clers S, Jackson GD, O’Dor RK, Pauly D, Purchase LV, Roberts MJ (1998) Cephalopod fisheries: a future global upside to past overexploitation of living marine resources? Results of an International Workshop, 31 August–2 September 1997, Cape Town. S Afr J Mar Sci 20(1):463–469

    Article  Google Scholar 

  • Martins RS, Perez JAA (2007) The ecology of loliginid squid in shallow waters around Santa Catarina Island, southern Brazil. Bull Mar Sci 80(1):125–145

    Google Scholar 

  • Matsuura Y (1986) Contribuição ao estudo da estrutura oceanográfica da região sudeste entre Cabo Frio (RJ) e Cabo de Santa Marta Grande (SC). Cienc Cult 38(8):1439–1450

    Google Scholar 

  • Migotto AE, Vervoort W (1996) Die Benthic shallow-water hydroids (Cnidaria, Hydrozoa) of the coast of São Sebastião, Brazil, including a checklist of Brazilian hydroids. Zool Verh 306:5–6

    Google Scholar 

  • Neige P, Boletzky SV (1997) Morphometrics of the shell of three Sepia species (Mollusca: Cephalopoda): intra- and interspecific variation. Zool Beitr 38:137–156

    Google Scholar 

  • Nigmatullin CM, Nesis KN, Arkhipkin AI (2001) A review of the biology of the jumbo squid Dosidicus gigas (Cephalopoda: Ommastrephidae). Fish Res 54(1):9–19

    Article  Google Scholar 

  • O’Dor RK (1988) The forces acting on swimming squid. J Exp Biol 137:421–442

    Google Scholar 

  • Palma ED, Matano RP, Piola AR (2008) A numerical study of the Southwestern Atlantic Shelf circulation: stratified ocean response to local and offshore forcing. J Geophys Res 113:C11010. doi:10.1029/2007JC004720

    Article  Google Scholar 

  • Pierce GJ, Guerra A (1994) Stock assessment methods used for cephalopod fisheries. Fish Res 21(1):255–285

    Article  Google Scholar 

  • Pierce GJ, Thorpe RS, Hastie LC, Brierley AS, Guerra A, Boyle PR, Jamieson R, Avila P (1994) Geographic variation in Loligo forbesi in the Northeast Atlantic Ocean: analysis of morphometric data and tests of causal hypotheses. Mar Biol 119(4):541–547

    Article  Google Scholar 

  • Pineda SE, Hernández DR, Bruneit NE (1998) Statolith comparison of two south-west Atlantic loliginid squid: Loligo sanpaulensis and Loligo gahi. S Afr J Mar Sci 20(1):347–354

    Article  Google Scholar 

  • Pineda SE, Hernández DR, Brunetti NE, Jerez B (2002) Morphological identification of two southwest Atlantic loliginid squids: Loligo gahi and Loligo sanpaulensis. [Identificación morfológica de los calamares loligínidos del Atlántico sudoccidental: Loligo gahi y Loligo sanpaulensis]. Rev Invest Desarr Pesq 15:67–84

    Google Scholar 

  • Rabinovich JE (1978) Ecología de poblaciones animales. In: Serie de biología, vol. 21. OEA

  • Richtsmeier JT, Burke Deleon V, Lele SR (2002) The promise of geometric morphometrics. Am J Phys Anthropol 119(S35):63–91

    Article  Google Scholar 

  • Rivas AL (1990) Heat-balance and annual variation of mean temperature in the north-patagonian gulfs. Oceanol Acta 13(3):265–272

    Google Scholar 

  • Rivas AL, Beier EJ (1990) Temperature and salinity fields in the north patagonian gulfs. Oceanol Acta 13(1):15–20

    Google Scholar 

  • Rodhouse PG (2005) C2. World squid resources. Rev State World Mar Fish, pp 175–187

  • Rodhouse PG, Hatfield EMC (1992) Production of soma and gonad in maturing male Illex argentinus (Mollusca: Cephalopoda). J Mar Biol Assoc U K 72(02):293–300

    Article  Google Scholar 

  • Rodhouse PG, Pierce GJ, Nichols OC, Sauer WH, Arkhipkin AI, Laptikhovsky VV, Lipinski MR, Ramos J, Gras M, Kidokoro H, Sadayasu K (2014) Environmental effects on cephalopod population dynamics: implications for management of fisheries. Adv Mar Biol 67:99–223

    Article  PubMed  Google Scholar 

  • Rodrigues AR, Gasalla MA (2008) Spatial and temporal patterns in size and maturation of Loligo plei and Loligo sanpaulensis (Cephalopoda: Loliginidae) in southeastern Brazilian waters, between 23°S and 27°S. Sci Mar 72(4):631–643

    Article  Google Scholar 

  • Rohlf FJ (2016) TPS utility program version 1.70. Department of Ecology and Evolution, State University of New York, Stony Brook, NY

  • Ruiz AE, Mendia LF (2008) Observaciones morfométricas y reproductivas en el langostino Pleoticus muelleri Bate, 1888 procedente de embarcaciones comerciales del Puerto Rawson, Argentina. Rev Biol Mar Ocean 43(3):675–680

    Google Scholar 

  • Sinn DL, Gosling SD, Moltschaniwskyj NA (2008) Development of shy/bold behaviour in squid: context-specific phenotypes associated with developmental plasticity. Anim Behav 75(2):433–442

    Article  Google Scholar 

  • Soares I, Möller O (2001) Low-frequency currents and water mass spatial distribution on the southern Brazilian shelf. Cont Shelf Res 21(16):1785–1814

    Article  Google Scholar 

  • Tonini MH, Palma ED, Piola AR (2013) A numerical study of gyres, thermal fronts and seasonal circulation in austral semi-enclosed gulfs. Cont Shelf Res 65:97–110

    Article  Google Scholar 

  • Toro Ibacache MV, Manriquez Soto G, Suazo Galdames I (2010) Morfometría geométrica y el estudio de las formas biológicas: de la morfología descriptiva a la morfología cuantitativa. Int J Morphol 28(4):977–990

    Article  Google Scholar 

  • Van Der Vyver JSF, Sauer WHH, McKeown NJ, Yemane D, Shaw PW, Lipinski MR (2015) Phenotypic divergence despite high gene flow in chokka squid Loligo reynaudii (Cephalopoda: Loliginidae): implications for fishery management. J Mar Biol Assoc U K 96:1507–1525

    Article  Google Scholar 

  • Vidal EAG, Marian JEAR, Martins RS (2013) Chapter 9. Doryteuthis sanpaulensis, São Paulo squid. In: Rosa R, O’Dor R, Pierce G (eds) Advances in squid biology, ecology and fisheries. Part I—myopsid squids, 1st edn. Nova Science Publishers, New York, pp 257–300

    Google Scholar 

  • Vigliano PH (1985) Contribución al conocimiento de la biología de Loligo brasiliensis Blainville 1823 (Mollusca: cephalopoda) en aguas argentinas. Dissertation, Universidad Nacional de la Plata

  • Zaleski T, Perez JAA, Gandara-Martins AL (2012) Morphological and morphometric variability of the squid Lolliguncula brevis (Mollusca: Cephalopoda) in Brazilian waters: evidence for two species in the western atlantic? An Acad Bras Cienc 84(4):1015–1028

    Article  PubMed  Google Scholar 

  • Zelditch ML, Fink WL, Swiderski DL, Lundrigan BL (1998) On applications of geometric morphometrics to studies of ontogeny and phylogeny: a reply to Rohlf. Syst Biol 47(1):159–167

    Article  Google Scholar 

  • Zelditch ML, Swiderski DL, Sheets HD, Fink WL (2004) Geometric morphometrics for biologists: a primer. Chapter 1. Elsevier Academic Press, New York, pp 6–20

    Google Scholar 

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Acknowledgements

We thank Dr. Felippe Postuma and Cooperpescass, Cooperativa de Pesca de São Sebastião, SP, Brazil for their help in the sampling of the specimens mentioned in this manuscript. Special thanks go to the reviewers who helped to improve the manuscript.

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Authors and Affiliations

  1. Instituto de Biología de Organismos Marinos, IBIOMAR-CONICET, Blvd. Brown 2915, 9120, Puerto Madryn, Chubut, Argentina

    Ramiro Braga, Silvina Van der Molen & Nicolás Ortiz

  2. Centro de Estudio de Sistemas Marinos, CESIMAR-CONICET, Blvd. Brown 2915, 9120, Puerto Madryn, Chubut, Argentina

    Augusto César Crespi-Abril

  3. Universidad Nacional del Comahue, Escuela Superior de Ciencias Marinas, Güemes 1030, 8520, San Antonio Oeste, Río Negro, Argentina

    Augusto César Crespi-Abril

  4. Universidad Nacional de la Patagonia San Juan Bosco, Blvd. Brown 3051, 9120, Puerto Madryn, Chubut, Argentina

    Augusto César Crespi-Abril

  5. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Campus Carreiros. Av. Itália, km 8, 96203-900, Rio Grande, RS, Brazil

    Mara Cristiane Rodrigues Silveira Bainy

  6. Facultad Regional Chubut, Universidad Tecnológica Nacional, Av. del Trabajo 1536, 9120, Puerto Madryn, Chubut, Argentina

    Nicolás Ortiz

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  1. Ramiro Braga
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Correspondence to Augusto César Crespi-Abril.

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Communicated by Y. Sakurai

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Braga, R., Crespi-Abril, A.C., Van der Molen, S. et al. Analysis of the morphological variation of Doryteuthis sanpaulensis (Cephalopoda: Loliginidae) in Argentinian and Brazilian coastal waters using geometric morphometrics techniques. Mar Biodiv 47, 755–762 (2017). https://doi.org/10.1007/s12526-017-0661-z

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