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Functional characterization of novel phosphate solubilizing bacteria, Chryseomicrobium imtechense, for enhanced strawberry growth and yield parameters

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Abstract

The strawberries are important for their economic and commercial values. The phosphate solubilizing bacteria (PSB) are associated with the roots (rhizomicrobiome). The present study was intended to isolate and characterize the potential PSB with other functional attributes along with its effect on growth and yield of the strawberry cultivar (Fragaria — ananassa Duch). Potential P-soulbilizers were isolated from the rhizospheric soil collected from agricultural fields. Isolates were screened and reveals increased production of siderophore, HCN, ammonia, and IAA along with antagonistic property. 16S rRNA gene was amplified for homology analysis and identification of the selected strain. Field trial of SS-4 on strawberry was conducted, and various physcio-chemical parameters such as plant height, plant spread, number of leaves, number of flowers, and fruits along with fruit yield were carried out using standard protocols. Out of four bacterial isolates, SS-4 was selected, characterized, and identified as Chryseomicrobium imtechense (accession number: JN967653). Significant improvement was recorded in all the physcio-chemical parameters of strawberry cultivar (Fragaria × ananassa Duch). Potential and novel plant growth promoting Rhizobacteria (PGPR), Chryseomicrobium imtechense, has been isolated and successfully applied in the field. This isolate may be explored in the future for other crops.

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References

  • Abd El-Rahman AF, Shaheen HA, Abd El-Az RM et al (2019) Influence of hydrogen cyanide-producing rhizobacteria in controlling the crown gall and root-knot nematode, Meloidogyne incognita. Egypt J Biol Pest Control 29:1–11

    Article  Google Scholar 

  • Ali MA, Yang L, Hafeez R, Li X, Hossain A, Xie T, Lin LB, Yin Y, Yan J, An Q (2021) Functional analysis and genome mining reveal high potential of biocontrol and plant growth promotion in nodule-inhabiting bacteria within Paenibacillus polymyxa complex. Front Microbiol 11:618601

    Article  Google Scholar 

  • Altschul SF, Thomas LM, Alejandro AS (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25:3389–3402

    Article  Google Scholar 

  • Anand K, Kumari B, Mallick MA (2016) Phosphate solubilizing microbes: an effective and alternative approach as biofertilizers. Int J Pharm Pharm Sci 8:37–40

    Google Scholar 

  • AOAC (1998) Official Method of Analysis, 15th edn. Association of Official Analytical Chemists, Washington DC

    Google Scholar 

  • Behera B, Yadav H, Singh S, Mishra R, Sethi B, Dutta S et al (2017) Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha. India J Gene Eng Biotechnol 15:169–178

    Article  Google Scholar 

  • Benson DA, Karsch-Mizrachi I, Lipmann DJ, Ostell J, Wheeler DL (2006) GenBank. Nucleic Acids Res 34:6–20

    Article  Google Scholar 

  • Berg G, Grube M, Schloter M, Smalla K (2014) Unraveling the plant microbiome: looking back and future perspectives. Front Microbiol 5:148

    Article  Google Scholar 

  • Bhatt K, Maheshwari DK (2019) Decoding multifarious role of cow dung bacteria in mobilization of zinc fractions along with growth promotion of C. annuum L. Sci Rep 9:14232

    Article  Google Scholar 

  • Bhatt K, Maheshwari DK (2019) Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L. 3. Biotech 10(2):36

    Google Scholar 

  • Bhatt K, Maheshwari DK (2020) Zinc solubilizing bacteria (Bacillus megaterium) with multifarious plant growth promoting activities alleviates growth in Capsicum annuum L. 3. Biotech 10:36

    Google Scholar 

  • Bindraban PS, Dimkpa CO, Pandey R (2020) Exploring phosphorus fertilizers and fertilization strategies for improved human and environmental health. Biol Fertil Soils 56(3):299–317

    Article  Google Scholar 

  • Budzikiewicz H (1993) Secondary metabolites from fluorescent Pseudomonads. Microbiol Rev 104:209–228

    Google Scholar 

  • Bulgarelli D, Garrido-Oter R, Münch PC, Weiman A, Dröge J, Pan Y, McHardy AC, Schulze-Lefert P (2015) Structure and function of the bacterial root microbiota in wild and domesticated barley. Cell Host Microbe 17:392–403

    Article  Google Scholar 

  • Cappuccino JC, Sherman N (2005) Microbiology: a laboratory manual, New York 125–179

  • Cervantes C, Chavez J, Cardova A, Velasco JA et al (1986) 20060 Resistance to metal by Pseudomonas aeruginosa clinical isolates. Microbiol 48:159–163

    Google Scholar 

  • Chauhan A, Saini R, Sharma JC (2020) Plant growth promoting rhizobacteria and their biological properties for soil enrichment and growth promotion. J Plant Nutri 45:277–293

    Google Scholar 

  • Chen Q, Liu S (2019) Identification and characterization of the phosphate-solubilizing bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi China. Front Microbiol 10:2171

    Article  Google Scholar 

  • Cole JR, Chai B, Farris RJ, Wang Q (2007) The Ribosomal Database Project (RPD-II): introducing my RDP space and quality controlled public data. Nucl Acids Res 35:169–172

    Article  Google Scholar 

  • Dastager SG, Deepa C, Pandey A (2010) Isolation and characterization of novel plant growth promoting Micrococcus sp NII-0909 and its interaction with cowpea. Plant Physiol Biochem 48:987–992

    Article  Google Scholar 

  • Donate-Correa J, Leon-Barrios M, Perez- Galdona R (2004) Screening for plant growth promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree shrub legume endemic to the Canary Island. Plant Soil 266:261–272

    Article  Google Scholar 

  • Dudley KJ, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis software-Version 4.0. Mol Bio Evol 24:1596–1599

    Article  Google Scholar 

  • Etminan M, Takkouche B, Caamano-Isorna F (2004) The role of tomato products and lycopene in the prevention of prostate cancer: a meta-analysis of observational studies. Cancer Epidemiol Biomarkers Prev 13:340–345

    Article  Google Scholar 

  • Franco-Franklin V, Moreno-Riascos S, Ghneim-Herrera T (2020) Are endophytic bacteria an option for increasing heavy metal tolerance of plants? A meta-analysis of the effect size. Front Environ Sci 8:603668

    Article  Google Scholar 

  • Giampieri F, Tulipani S, Alvarez-Suarez JM, Quiles JL, Mezzetti B, Battino M (2012) The strawberry: composition, nutritional quality, and impact on human health. Nutrition 28:9–19

    Article  Google Scholar 

  • Glickman E, Dessaux Y (1995) A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl Environ Microbiol 61:793–796

    Article  Google Scholar 

  • Gopalakrishnan S, Pande S, Sharma M, Humayun P, Kiran BK, Sandeep D, Vidya MS, Deepthi K, Rupela O (2011) Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea. Crop Protect 30:1070–1078

    Article  Google Scholar 

  • Hussein KA, Joo JH (2014) Potential of siderophore production by bacteria isolated from heavy metal: polluted and rhizosphere soils. Curr Microbiol 68:717–723

    Article  Google Scholar 

  • Jha A, Sharma D, Saxena J (2012) Effect of single and dual phosphate solubilizing bacterial strain inoculations on overall growth of mung bean plants. Arch Agron Soil Sci 58:967–981

    Article  Google Scholar 

  • Jiang Z, Zhang X, Wang Z, Cao B, Deng S, Bi M et al (2019) Enhanced biodegradation of atrazine by Arthrobacter sp. DNS10 during co-culture with a Phosphorus solubilizing bacteria: Enterobacter sp. P1. Ecotoxicol Environ Safety 172:159–166

    Article  Google Scholar 

  • Johnsen SP, Overvad K, Stripp C, Tjonneland A, Husted SE, Sorensen HT (2003) Intake of fruit and vegetables and the risk of ischaemic stroke in a cohort of Danish men and woman. Am J Clin Nutr 78:57–64

    Article  Google Scholar 

  • Jones DA, Smith BFL, Wilson MJ, Goodman BA (1991) Solubilisator fungi of phosphate in rice soil. Mycol Res 95:1090–1093

    Article  Google Scholar 

  • Kannapiran E, Ravindran J (2012) Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar. J Basic Microbiol 52:91–98

    Article  Google Scholar 

  • Korir H, Mungai NW, Thuita M, Hamba Y, Masso C (2017) Co-inoculation effect of rhizobia and plant growth promoting rhizobacteria on common bean growth in a low Phosphorus soil. Front Plant Sci 8:1–10

    Article  Google Scholar 

  • Kumar A, Cameotra SS, Gupta S (2012) Screening and characterization of potential cadmium biosorbent Alcaligenes strain from industrial effluent. J Basic Microbiol 52:160–166

    Article  Google Scholar 

  • Kumar P, Dubey RC, Maheshwari DK (2012) Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens. Microbiol Res 167(8):493–499

    Article  Google Scholar 

  • Lundberg DS, Lebeis SL, Paredes SH, Yourstone S, Gehring J, Malfatti S, Tremblay J, Engelbrektson A, Kunin V, Del Rio TG, Edgar RC, Eickhorst T, Ley RE, Hugenholtz P, Tringe SG, Dangl JL (2012) Defining the core Arabidopsis thaliana root microbiome. Nature 488:86–90

    Article  Google Scholar 

  • Lynch JM (1985) Origin, nature and biological activity of aliphatic substances and growth hormones found in soil. In: Vaughan D, Marcolm RE (ed) Soil organic matter and biological activity. Developments in Plant and Soil Sciences. Springer, Dordrecht 16:151–174

  • Marques APGC, Pires C, Moreira H, Rangel AOSS, Castro PML (2010) Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biol Biochem 42:1229–1235

    Article  Google Scholar 

  • Mei X, Wang Y, Li Z, Larousse M, Pere A, da Rocha M, Zhan F, He Y, Pu L, Panabières F, Zu Y (2021) Root-associated microbiota drive phytoremediation strategies to lead of Sonchus asper (L) Hill as revealed by intercropping-induced modifications of the rhizosphere microbiome. Environ Sci Pollut. https://doi.org/10.1007/s11356-021-17353-1

    Article  Google Scholar 

  • Naik PR, Raman G, Narayanan KB, Sakthivel N (2008) Assessment of genetic and functional diversity of phosphate solubilizing fluorescent Pseudomonads isolated from rhizospheric soil. BMC Microbiol 8:230

    Article  Google Scholar 

  • Nartey LK, Pu QP, Zhu W, Zhang S, Li Z, Yao Y, Hu X (2022) Antagonistic and plant growth promotion effects of Mucor moelleri, a potential biocontrol agent. Microbiol Res 255:126922

    Article  Google Scholar 

  • Nesme T, Metson GS, Bennett EM (2018) Global Phosphorus flows through agricultural trade. Glob Environ Change 50:133–141

    Article  Google Scholar 

  • Olander LP, Vitousek PM (2000) Regulation of soil phosphatase and chitinase activity by N and P availability. Biogeochem 49:175–190

    Article  Google Scholar 

  • Otieno N, Lally RD, Kiwanuka S, Lloyd A, Ryan D, Germaine KJ, Dowling DN (2015) Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Front Microbiol 6:1–9

    Google Scholar 

  • Paul D, Sinha SN (2017) Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga. India Ann Agrarian Sci 15:130–136

    Article  Google Scholar 

  • Proteggente AR, Saija A, De Pasquale A, Rice-Evans CA (2003) The compositional characterisation and antioxidant activity of fresh juices from Sicilian sweet orange (Citrus sinensis L. Osbeck) varieties. Free Radic Res 37:681–687

    Article  Google Scholar 

  • Rafique M, Sultan T, Ortas I, Chaudhary HJ (2017) Enhancement of maize plant growth with inoculation of phosphate-solubilizing bacteria and biochar amendment in soil. Soil Sci Plant Nutr 63:460–469

  • Rahman A, Sitepu IR, Tang SY, Hashidoko Y (2010) Salkowski’s reagent test as a primary screening index for functionalities of rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil. Biosci Biotechnol Biochem 74:2202–2208

    Article  Google Scholar 

  • Ranganna S (2007) Handbook of analysis and quality control for fruits and vegetable products, 2nd edn. Tata McGraw Hill Publication Co., Ltd, New Delhi

    Google Scholar 

  • Rijavec T, Lapanje A (2016) Hydrogen cyanide in the rhizosphere: not suppressing plant pathogens, but rather regulating availability of phosphate. Front Microbiol 7:1785

    Article  Google Scholar 

  • RodriGuez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339

    Article  Google Scholar 

  • Sambrook J, Russell DW (2011) Molecular cloning: a laboratory manual. Cold spring Harbor Laboratory Press, New York

    Google Scholar 

  • Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino M (2005) Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nutrition 21:207–213

    Article  Google Scholar 

  • Schnug E, Haneklaus SH (2016) The enigma of fertilizer phosphorus utilization. In: Schnug E, De Kok LJ (eds) Phosphorus in agriculture: 100% zero. Springer, Dordrecht, pp 7–26

    Google Scholar 

  • Schwyn B, Neiland JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56

    Article  Google Scholar 

  • Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA (2013) Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. Springerplus 2:587–600

    Article  Google Scholar 

  • Skidmore AM, Dickinson CH (1976) Colony interactions and hyphal interference between Septoria nodorum and phylloplane fungi. Trans Br Mycol Soc 66:57–64

    Article  Google Scholar 

  • Smith DL, Subramanian S, Lamont JR, Bywater-Ekegard M (2015) Signaling in the phytomicrobiome: breadth and potential. Front Plant Sci 6:709

    Article  Google Scholar 

  • Tahir HAS, Gu Q, Wu H, Raza W, Hanif A, Wu L, Colman MV, Gao X (2017). Plant growth promotion by volatile organic compounds produced by Bacillus subtilis SYST2. Front. Microbiol 8:171

  • Taiz L, Zeiger E (1998) Plant physiology, 2nd edn. Sinauer Associates Publishers, Sunderland, Massachusetts

    Google Scholar 

  • Thomas S, Mathew L, Rishad K (2018) Isolation and molecular identification of phosphate solubilizing bacteria, Bacillus licheniformis UBPSB-07 capable of enhancing seed germination in Vigna radiata L. Phytomorphol 68:13–18

    Google Scholar 

  • Thomson JD, Gibson HDG, TJ, (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  Google Scholar 

  • Tulipani S, Mezzetti B, Capocasa F, Bompadre S, Beekwilder J, deVos CH, Capanoglu E, Bovy A, Battino M (2008) Antioxidants, phenolic compounds, and nutritional quality of different strawberry genotypes. J Agr Food Chem 56(694):704

    Google Scholar 

  • Turner TR, James EK, Poole PS (2013) The plant microbiome. Genome Biol 14:209

  • Valetti L, Iriarte L, Fabra A (2018) Growth promotion of rapeseed (Brassica napus) associated with the inoculation of phosphate solubilizing bacteria. Appl Soil Ecol 132:1–10

    Article  Google Scholar 

  • Vauzour D, Vafeiadou K, Pendeiro C, Corona G, Spencer JPE (2010) The inhibitory effects of berryderivated flavonoids against neurodegenerative processes. J Berry Res 1:45–52

    Article  Google Scholar 

  • Viruel E, Erazzú L, Calsina L, Ferrero M, Lucca M, Sineriz F (2013) Inoculation of maize with phosphate solubilizing bacteria: Effect on plant growth and yield. J Soil Sci Plant Nutr 14

  • Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naïve Bayesian classifier for rapid assignment of r RNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73:5261–5267

    Article  Google Scholar 

  • Wang Y, Peng S, Hua Q, Qiu C, Wu P, Liu X, Lin X (2021) The long-term effects of using phosphate-solubilizing bacteria and photosynthetic bacteria as biofertilizers on peanut yield and soil bacteria community. Front Microbiol 12:693535

  • Wang SY, Lin HS (2000) Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. J Agr Food Chem 48:140–146

    Article  Google Scholar 

  • Whitelaw MA, Harden TJ, Helyar KR (1999) Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biol Biochem 32:655–665

  • Xu W, Wang F, Zhang M, Ou T, Wang R, Strobel G, Xiang Z, Zhou Z, Xie J (2019) Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities. Microbiol Res 229:126328

    Article  Google Scholar 

  • Yu Q, Ma Z, Wang J, Wei L (2012) Screening of phosphorus solubilizing bacteria and its effects on transformation of soil inorganic Phosphorus fractions. J Soil Water Conserv 26:103–107

    Google Scholar 

  • Zhu F, Qu L, Hong X, Sun X (2011) Isolation and characterization of a phosphate solubilizing halophilic bacterium Kushneria sp YCWA18 from Daqiao Saltern on the coast of yellow sea of China. Evid Based Complement Alternat Med 2011:615032

    Article  Google Scholar 

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Acknowledgements

The authors thank the Director Principal, Mata Gujri College, for providing field and necessary laboratory facilities to carryout current experiments.

Funding

The authors received financial support from the Department of Biotechnology, Ministry of Science and Technology, Government of India, under Star College Scheme (Ref. No. 102/IFD/SAN/543/2019–20).

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Authors and Affiliations

  1. Department of Microbiology, Mata Gujri College, Fatehgarh Sahib, Punjab, India, 140406

    Saurabh Gupta, Upinderjit Kaur & Jhilmil Singh

  2. Department of Agriculture, Mata Gujri College, Fatehgarh Sahib, Punjab, India, 140406

    Dilip Singh Kachwaya & Ravindra Kumar

  3. Department of Botany, Mata Gujri College, Fatehgarh Sahib, Punjab, India, 140406

    Vijay Singh

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  1. Saurabh Gupta
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  2. Shanu Bansal
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Gupta, S., Bansal, S., Kaur, U. et al. Functional characterization of novel phosphate solubilizing bacteria, Chryseomicrobium imtechense, for enhanced strawberry growth and yield parameters. Arab J Geosci 15, 1119 (2022). https://doi.org/10.1007/s12517-022-10102-9

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