Abstract
A bidirectional promoter is an intergenic region between a pair of adjacent and oppositely transcribed genes (‘head-to-head’ genes) that concurrently initiates the expression of both genes. In this work, we identified a bidirectional promoter PBn265 with a 265 bp sequence between transcription initiation codons (ATG) of two genes encoding the homeodomain protein SHH2 and chloroplast GROUP II intron splicing factor CFM3, respectively, in genome of Brassica napus. The histochemical staining and fluorescence microscopy results demonstrated that PBn265 possess bidirectional promoting activity in the various tissues of the transgenic Arabidopsis thaliana. Notably, the bidirectional expression activities of PBn265 were significantly increased following red or blue light induction. Furthermore, the gene structure, ‘SHH2-BiP-CFM3’, appears highly conservative among various species in Brassicaceae. Considering its short sequence length, high sequence conservation and light-inducible properties, this promoter from Brassica napus most likely has application potential in bioengineering and agricultural molecular breeding.
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Abbreviations
- SHH2 :
-
SAWADEE homeodomain homolog 2
- CRM:
-
The chloroplast RNA splicing and ribosome maturation
- CFM3 :
-
CRM family member 3
- eGFP :
-
Enhanced green fluorescent protein
- GUS :
-
β-Glucuronidase
- BiP:
-
Bidirectional promoter
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Acknowledgements
The authors would like to express their gratitude to EditSprings (https://www.editsprings.com/) for the expert linguistic services provided.
Funding
This work was supported by The National Key Research and Development Plan (2016YFD0100202 and 2018YFD0100501) and Sichuan Province Breeding Project (2021YFYZ0018).
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RZ: conceptualization, methodology, writing—original draft, investigation. YF: part of assays, data curation. LZ: formal analysis, validation. TW: methodology, writing–review and editing. XJ: methodology. MW: investigation, resources, writing–review and editing, project administration, funding acquisition.
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Zhu, R., Fu, Y., Zhang, L. et al. A Light-Inducible Bidirectional Promoter Initiates Expression of Both Genes SHH2 and CFM3 in Brassica napus L.. J. Plant Biol. 66, 475–484 (2023). https://doi.org/10.1007/s12374-022-09367-0
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DOI: https://doi.org/10.1007/s12374-022-09367-0