Abstract
Anthocyanins are natural pigments and play significant roles in multiple growth, development, and stress response processes in plants. The vegetables with high anthocyanin content have better colours, higher antioxidant activity than green vegetables and are potent antioxidants with health benefits. However, the mechanism of anthocyanin accumulation in purple and green leaves of Raphanus sativus (radish) is poorly understood and needs further investigation. In the present study, the pigment content in a green leaf cultivar “RA9” and a purple-leaf cultivar “MU17” was characterized and revealed that the MU17 had significantly increased accumulation of anthocyanins and reduced content of chlorophyll and carotenoid compared with that in RA9. Meanwhile, these two cultivars were subjected to a combination of metabolomic and transcriptome studies. A total of 52 massively content-changed metabolites and 3463 differentially expressed genes were discovered in MU17 compared with RA9. In addition, the content of significantly increased flavonoids (such as pelargonidin and cyanidin) was identified in MU17 compared to RA9 using an integrated analysis of metabolic and transcriptome data. Moreover, the quantitative real-time polymerase chain reaction results also confirmed the differences in the expression of genes related to pathways of flavonoids and anthocyanin metabolism in MU17 leaves. The present findings provide valuable information for anthocyanin metabolism and further genetic manipulation of anthocyanin biosynthesis in radish leaves.
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Data availability
The RNA-seq raw data for the current study were deposited in the National Genomics Data Center (https://ngdc.cncb.ac.cn/gsa/browse/CRA008485) under accession ID CRA008485.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 4111800096); Nanchong applied technology research and development program (No. 20YFZJ0076); Nanchong research and development Project (No. 21YFZJ0041); The funds for the talents introduction program of Guizhou University of China (No. (2020)40).
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Pu, Q., He, Z., Xiang, C. et al. Integration of metabolome and transcriptome analyses reveals the mechanism of anthocyanin accumulation in purple radish leaves. Physiol Mol Biol Plants 28, 1799–1811 (2022). https://doi.org/10.1007/s12298-022-01245-w
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DOI: https://doi.org/10.1007/s12298-022-01245-w