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Hesperidin Supplementation Improves Altered PON -1, LDL Oxidation, Inflammatory Response and Hepatic Function in an Experimental Rat Model of Hyperlipidemia

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Abstract

In this study, we have examined the effect of hesperidin on rats fed on an experimental high-fat diet. Male Wistar rats were given a high-fat diet orally for one month for developing an HFD (High fat- diet) model. Rats were also supplemented with hesperidin (100 mg/kg body weight) for one month. We determined serum LDL (Low-density lipoprotein) oxidation, Paraoxonase-1 (PON-1) activity, and histopathological profile of the liver. Inflammatory cytokines levels were also measured in serum. HFD induced significant changes in LDL oxidation and PON-1 activity. Liver tissue histopathology and gene expression of inflammatory markers (Il-6(Interleukin-6), TNF- alpha (Tumor necrosis factor alpha), NF-KB (Nuclear factor kappa B) show that significant changes occur in the hyperlipidemic model of rats. We also show that hesperidin can effectively improve plasma antioxidant, LDL oxidation, and inflammatory cytokine expression in rats already subjected to hyperlipidemic stress. We conclude that hesperidin may protect the liver from oxidative stress by improving hepatic function.

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Data Availability

The information that helps the finding of this study is accessible from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Biochemistry, University of Allahabad, Allahabad, 211002, India

    Raushan Kumar & Syed Ibrahim Rizvi

  2. Department of Clinical Nutrition, College of Applied Health Sciences in Ar Rass, Qassim University, Ar Rass, 51921, Saudi Arabia

    Mohammad Idreesh Khan

  3. Clinical Nutrition Department, Applied Medical Sciences College, Jazan University, Jazan, 82817, Saudi Arabia

    Fauzia Ashfaq & Abdulrahman A. Alsayegh

  4. Department of Biochemistry, College of Medicine, University of Hail, Hail, Saudi Arabia

    Fahmida Khatoon

  5. Department of Family and Community Medicine, College of Medicine, University of Hail, Hail, Saudi Arabia

    Tahani Nasser Altamimi

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  1. Raushan Kumar
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Correspondence to Syed Ibrahim Rizvi.

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All animal care and exploratory methods conformed with the guidelines of the Control and Supervision of Experiments on Animals (CPCSEA) and Institutional Animal Ethics Committee (IAEC), University of Allahabad, India.

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Kumar, R., Khan, M.I., Ashfaq, F. et al. Hesperidin Supplementation Improves Altered PON -1, LDL Oxidation, Inflammatory Response and Hepatic Function in an Experimental Rat Model of Hyperlipidemia. Ind J Clin Biochem 39, 257–263 (2024). https://doi.org/10.1007/s12291-023-01140-5

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