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Effect of Abutilon indicum (L) Extract on Adipogenesis, Lipolysis and Cholesterol Esterase in 3T3-L1 Adipocyte Cell Lines

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Abstract

Abutilon indicum (L) is an Indian traditional plant used for the treatment of diabetes and heart diseases. The present study is to evaluate the functional of A. indicum leaf extract as insulin like character to inhibit lipolysis and stimulates Adipogenesis activity. The ability of the A. indicum leaf extract in anti-obesity effect of Adipogenesis, lipolysis and cholesterol esterase functions can be predicted by using 3T3-L1 adipocyte cell lines. Substances were isolated from A. indicum leaves and the double filtered crude sample were used for Adipogenesis, lipolysis and cholesterol esterase activity using 3T3-L1 adipocytes at different concentrations. We used differential media-I, differential media-II and maintenance media (MM1) at concentrations of 20, 40, 60, 80, 100, 200 and 400 µg/mL respectively. In addition to the extract, there is a significance increase in glycerol release (p < 0.001) compared with crude and reference compounds. Cholesterol esterase activity predicts the IC50 = 27.11 µg/mL of orlistat positive control compare with IC50 = 8.158 µg/mL of crude extract. Based on the observation, A. indicum leaf extract can promotes lipolysis and differentiated adipocytes. It is potentially used as adjuvant in the treatment of Type 2 diabetes.

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Acknowledgements

I would like to thank Dr. V. Veeraraghavan, research guide for supporting overall design of the experiment. C. N. Prashantha and K. Gouthami have helped to compile the results and supported for statistical calculation. I also thank Dr. Renuka Srihari for providing the laboratory support to conduct analytical experiments to generate research data.

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Authors and Affiliations

  1. Department of Biochemistry, School of Applied Sciences, REVA University, Bangalore, 560064, India

    Lavanya Lakshminarayana, V. Veeraraghavan & Kuruvalli Gouthami

  2. Department of Biochemistry, Maharani Lakshmi Ammanni College for Women, Bangalore, 560012, India

    Renuka Srihari

  3. Department of Biotechnology, School of Applied Sciences, REVA University, Bangalore, 560064, India

    Prashantha Chowdadenahalli Nagaraja

Authors
  1. Lavanya Lakshminarayana
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  2. V. Veeraraghavan
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  3. Kuruvalli Gouthami
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  5. Prashantha Chowdadenahalli Nagaraja
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Corresponding author

Correspondence to V. Veeraraghavan.

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Authors declares that he has no conflict of interest.

Ethical Approval and Consent to Participate

The overall research is not used animals and is only in vitro based study.

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Human and Animal Rights

No Human cell are used only animals cell lines are use for the studies. Animal cell line are used Sigma Aldrich [ATCC-CL-173].

Research Resource Identifiers (RRID)

Product category: Animal cells

Organism: Mus musculus, mouse

Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

Cell type: fibroblast

Morphology: fibroblast Tissue Embryo

Applications 3D cell culture

Product format: Frozen Storage conditions Vapor phase of liquid nitrogen

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Lakshminarayana, L., Veeraraghavan, V., Gouthami, K. et al. Effect of Abutilon indicum (L) Extract on Adipogenesis, Lipolysis and Cholesterol Esterase in 3T3-L1 Adipocyte Cell Lines. Ind J Clin Biochem 38, 22–32 (2023). https://doi.org/10.1007/s12291-022-01022-2

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  • DOI: https://doi.org/10.1007/s12291-022-01022-2

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