Skip to main content

Advertisement

SpringerLink
Log in

Serum Angiopoietin Levels are Different in Acute and Chronic Myeloid Neoplasms: Angiopoietins do not only Regulate Tumor Angiogenesis

  • Original Article
  • Published:
Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Molecular balance between Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) has important effects in tumor angiogenesis. Ang-2 was shown to be elevated and proved to be a prognostic factor in acute myeloid leukemia (AML). To date studies revealed increased angiogenesis in bone marrows (BMs) of both myeloproliferative neoplasm (MPN) and AML patients. We conducted this study to demonstrate circulating levels of Ang-1 and Ang-2 in MPN patients since no data exists in literature. Thirty-three newly diagnosed MPN, 27 newly diagnosed AML patients and 25 controls (HC) were enrolled and Angiopoietin levels were determined with ELISA. We found that Ang-1 levels were higher whereas Ang-2 levels were lower in MPN and HC when compared to AML. Our results suggest that though angiogenesis is increased in both AML and MPN, angiopoietin serum level profile of the two diseases are different, and MPN patients have similar Ang-1 and Ang-2 levels as HC. We conclude that, according to our results Ang-1 and Ang-2 do not only regulate tumor angiogenesis and the difference between angiopoietin levels of acute and chronic myeloid neoplasms could be a reflection of other effects of these growth factors on tumor malignancy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Saharinen P, Eklund L, Pulkki K, Bono P, Alitalo K (2011) VEGF and angiopoietin signaling in tumor angiogenesis and metastasis. Trends Mol Med 17:347–362

    Article  CAS  PubMed  Google Scholar 

  2. Khosravi Shahi P, Fernández Pineda I (2008) Tumoral angiogenesis: review of the literature. Cancer Invest 26:104–108

    Article  PubMed  Google Scholar 

  3. Gianelli U, Vener C, Raviele PR, Savi F, Somalvico F, Calori R, Iurlo A, Radaelli F, Fermo E, Bucciarelli P, Bori S, Coggi G, Deliliers GL (2007) VEGF expression correlates with microvessel density in Philadelphia chromosome-negative chronic myeloproliferative disorders. Am J Clin Pathol 128:966–973

    Article  PubMed  Google Scholar 

  4. Lundberg LG, Lerner R, Sundelin P, Rogers R, Folkman J, Palmblad J (2000) Bone marrow in polycythemia vera, chronic myelocytic leukemia, and myelofibrosis has an increased vascularity. Am J Pathol 157:15–19

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Kvasnicka HM, Thiele J (2004) Bone marrow angiogenesis: methods of quantification and changes evolving in chronic myeloproliferative disorders. Histol Histopathol 19:1245–1260

    CAS  PubMed  Google Scholar 

  6. Hussong JW, Rodgers GM, Shami PJ (2000) Evidence of increased angiogenesis in patients with acute myeloid leukemia. Blood 95:309–313

    CAS  PubMed  Google Scholar 

  7. Dickson DJ, Shami PJ (2001) Angiogenesis in acute and chronic leukemias. Leuk Lymphoma 42:847–853

    Article  CAS  PubMed  Google Scholar 

  8. Alonci A, Allegra A, Bellomo G, Penna G, D’Angelo A, Quartarone E, Musolino C (2008) Evaluation of circulating endothelial cells, VEGF and VEGFR2 serum levels in patients with chronic myeloproliferative diseases. Hematol Oncol 26:235–239

    Article  CAS  PubMed  Google Scholar 

  9. Medinger M, Mross K (2010) Clinical trials with anti-angiogenic agents in hematological malignancies. J Angiogenes Res 2:10

    Article  PubMed  PubMed Central  Google Scholar 

  10. Musolino C, Alonci A, Bellomo G, Loteta B, Quartarone E, Gangemi D, Massara E, Calabrò L (2004) Levels of soluble angiogenin in chronic myeloid malignancies: clinical implications. Eur J Haematol 72:416–419

    Article  CAS  PubMed  Google Scholar 

  11. Shim WS, Ho IA, Wong PE (2007) Angiopoietin: a TIE(d) balance in tumor angiogenesis. Mol Cancer Res 5:655–665

    Article  CAS  PubMed  Google Scholar 

  12. Lee CY, Tien HF, Hu CY, Chou WC, Lin LI (2007) Marrow angiogenesis-associated factors as prognostic biomarkers in patients with acute myelogenous leukaemia. Br J Cancer 9:877–882

    Google Scholar 

  13. Schliemann C, Bieker R, Thoennissen N, Gerss J, Liersch R, Kessler T, Büchner T, Berdel WE, Mesters RM (2007) Circulating angiopoietin-2 is a strong prognostic factor in acute myeloid leukemia. Leukemia 21:1901–1906

    Article  CAS  PubMed  Google Scholar 

  14. Kümpers P, Koenecke C, Hecker H, Hellpap J, Horn R, Verhagen W, Buchholz S, Hertenstein B, Krauter J, Eder M, David S, Göhring G, Haller H, Ganser A (2008) Angiopoietin-2 predicts disease-free survival after allogeneic stem cell transplantation in patients with high-risk myeloid malignancies. Blood 112:2139–2148

    Article  PubMed  Google Scholar 

  15. Hou HA, Chou WC, Lin LI, Tang JL, Tseng MH, Huang CF, Yao M, Chen CY, Tsay W, Tien HF (2008) Expression of angiopoietins and vascular endothelial growth factors and their clinical significance in acute myeloid leukemia. Leuk Res 32:904–912

    Article  CAS  PubMed  Google Scholar 

  16. Tefferi A, Vardiman JW (2008) Classification and diagnosis of myeloproliferative neoplasms: the 2008 World Health Organization criteria and point-of-care diagnostic algorithms. Leukemia 22:14–22

    Article  CAS  PubMed  Google Scholar 

  17. Vardiman JW, Thiele J, Arber DA, Brunning RD, Borowitz MJ, Porwit A, Harris NL, Le Beau MM, Hellström-Lindberg E, Tefferi A, Bloomfield CD (2009) The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes. Blood 114:937–951

    Article  CAS  PubMed  Google Scholar 

  18. Quartarone E, Alonci A, Allegra A, Bellomo G, Calabrò L, D’Angelo A, Del Fabro V, Grasso A, Cincotta M, Musolino C (2006) Differential levels of soluble angiopoietin-2 and Tie-2 in patients with haematological malignancies. Eur J Haematol 77:480–485

    Article  CAS  PubMed  Google Scholar 

  19. Medinger M, Skoda R, Gratwohl A, Theocharides A, Buser A, Heim D, Dirnhofer S, Tichelli A, Tzankov A (2009) Angiogenesis and vascular endothelial growth factor-/receptor expression in myeloproliferative neoplasms: correlation with clinical parameters and JAK2-V617F mutational status. Br J Haematol 146:150–157

    Article  CAS  PubMed  Google Scholar 

  20. Mazzieri R, Pucci F, Moi D, Zonari E, Ranghetti A, Berti A, Politi LS, Gentner B, Brown JL, Naldini L, De Palma M (2011) Targeting the ANG2/TIE2 axis inhibits tumor growth and metastasis by impairing angiogenesis and disabling rebounds of proangiogenic myeloid cells. Cancer Cell 19:512–526

    Article  CAS  PubMed  Google Scholar 

  21. Scholz A, Lang V, Henschler R, Czabanka M, Vajkoczy P, Chavakis E, Drynski J, Harter PN, Mittelbronn M, Dumont DJ, Plate KH, Reiss Y (2011) Angiopoietin-2 promotes myeloid cell infiltration in a β2-integrin-dependent manner. Blood 118:5050–5059

    Article  CAS  PubMed  Google Scholar 

  22. Kim H, Koh GY (2011) Ang2, the instigator of inflammation. Blood 118:4767–4768

    Article  CAS  PubMed  Google Scholar 

Download references

Conflict of interest

The authors report no declarations of interest.

Author information

Authors and Affiliations

  1. Department of Hematology, Kocaeli University Hospital, Umuttepe, 41380, Kocaeli, Turkey

    Elif Birtas Atesoglu, Pinar Tarkun, Ozgur Mehtap, Esra Terzi Demirsoy & Abdullah Hacihanefioglu

  2. Department of Hematology, Baskent University, Istanbul, Turkey

    Figen Atalay

  3. Department of Hematology, Trakya University, Edirne, Turkey

    Muhammet Maden

  4. Department of Internal Medicine, Kocaeli University, Kocaeli, Turkey

    Koray Celebi

Authors
  1. Elif Birtas Atesoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pinar Tarkun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ozgur Mehtap
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Esra Terzi Demirsoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Figen Atalay
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhammet Maden
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Koray Celebi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Abdullah Hacihanefioglu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Elif Birtas Atesoglu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Atesoglu, E.B., Tarkun, P., Mehtap, O. et al. Serum Angiopoietin Levels are Different in Acute and Chronic Myeloid Neoplasms: Angiopoietins do not only Regulate Tumor Angiogenesis. Indian J Hematol Blood Transfus 32, 162–167 (2016). https://doi.org/10.1007/s12288-015-0548-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12288-015-0548-8

Keywords

Search

Navigation