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Multi-shell nanocomposites based multienzyme mimetics for efficient intracellular antioxidation

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Abstract

Oxidative stress is associated with many acute and chronic inflammatory diseases. Development of nanomaterial-based enzyme mimetics for reactive oxygen species (ROS) scavenging is challenging, but holds great promise for the treatment of inflammatory diseases. Herein, we report the highly ordered manganese dioxide encapsulated selenium-melanin (Se@Me@MnO2) nanozyme with high efficiency for intracellular antioxidation and anti-inflammation. The Se@Me@MnO2 nanozyme is sequentially fabricated through the radical polymerization and the in-situ oxidation-reduction. In vitro experimental results demonstrated that the Se@Me@MnO2 nanozyme exhibits multiple enzyme activities to scavenge ROS, including catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD). Mechanism researches illustrated that the Se core possesses GPx-like catalytic activity, the Me and the MnO2 possess both the SOD-like and the CAT-like activities. What’s more, due to the stable unpaired electrons existing in the nanozyme, the Se, Me and MnO2 provide synergistic and fast electron transfer effect to achieve the quickly scavenging of hydrogen peroxide, hydroxyl radical, and superoxide anion. Further in vivo experimental results showed that this biocompatible nanozyme exhibits cytoprotective effects by resisting ROS-mediated damage, thereby alleviating the inflammation. This multienzyme mimetics is believed to be an excellent ROS scavenger and have a good potential in clinical therapy for ROS-related diseases.

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Acknowledgements

This work was supported by the Innovation Zone Project (No. 18-163-12-ZT-003-077-01), Health Major Project (Nos. BWS17J028 and AWS16J018) and National Natural Science Foundation of China (Nos. 81872835, 21621003, and 21563010).

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Authors and Affiliations

  1. MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Beijing Key Lab of Microanalytical Methods & Instrumentation, Center for Synthetic and Systems Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China

    Yongjian Ai, Jinzhi You, Mingyu Ding & Qionglin Liang

  2. China Astronaut Research and Training Center, Beijing, 100094, China

    Jianyi Gao & Jiaping Wang

  3. Department of Chemistry, Northeastern University, Shenyang, 110819, China

    Hong-bin Sun

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  1. Yongjian Ai
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  2. Jinzhi You
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  4. Jiaping Wang
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  6. Mingyu Ding
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Correspondence to Qionglin Liang.

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Ai, Y., You, J., Gao, J. et al. Multi-shell nanocomposites based multienzyme mimetics for efficient intracellular antioxidation. Nano Res. 14, 2644–2653 (2021). https://doi.org/10.1007/s12274-020-3267-x

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