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Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 (fluoro)quinolones by one monoclonal antibody

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Abstract

For rapid and simultaneous detection of (fluoro)quinolones, a broadly specific monoclonal antibody (mAb) that recognizes 32 (fluoro)quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles (AuNPs) was then assembled with goat anti-mouse antibody and antigen (sarfloxacin coupled to ovalbumin), used to form the C line and T line, respectively. This antigen competes with the (fluoro)quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 (fluoro)quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1–10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of (fluoro)quinolones in milk.

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Acknowledgments

This work is financially supported by the National Natural Science Foundation of China (Nos. 21522102, 21503095, 21471068, 21371081, and 21301073), the Key Programs from MOST (Nos. 2016YFD0401101 and 2012YQ09019410), and grants from Natural Science Foundation of Jiangsu Province, MOF and MOE (Nos. BK20150145, BX20151038, BK20140003, BE2014672, BE2013613, BE2013611).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Juan Peng, Liqiang Liu, Liguang Xu, Shanshan Song, Hua Kuang, Gang Cui & Chuanlai Xu

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Juan Peng, Liqiang Liu, Liguang Xu, Shanshan Song, Hua Kuang, Gang Cui & Chuanlai Xu

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  1. Juan Peng
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  2. Liqiang Liu
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  5. Hua Kuang
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  6. Gang Cui
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  7. Chuanlai Xu
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Correspondence to Hua Kuang.

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Peng, J., Liu, L., Xu, L. et al. Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 (fluoro)quinolones by one monoclonal antibody. Nano Res. 10, 108–120 (2017). https://doi.org/10.1007/s12274-016-1270-z

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