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Optische Genregulation in Mikrobioreaktoren

  • Wissenschaft · Methoden
  • Optogenetik und Biotechnologie
  • Published:
BIOspektrum Aims and scope

Abstract

Photolabile-caged effector molecules allow non-invasive regulation of gene expression using cell-compatible UVA-irradiation. Based on this method, independent photoregulation of gene expression was combined with optical online monitoring of important cultivation parameters in a 48-well plate format. Thereby, an innovative screening system was created which allows for cost-effective and easily automated expression profiling as well as closed-loop process control.

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Authors and Affiliations

  1. Institut für Bioorganische Chemie, Heinrich-Heine-Universität Düsseldorf Forschungszentrum Jülich, D-52426, Jülich, Deutschland

    Peter M. Kusen & Jörg Pietruszka

  2. Aachener Verfahrenstechnik (AVT), Biochemical Engineering, RWTH Aachen, Aachen, Deutschland

    Kyra Hoffmann, Georg Wandrey & Jochen Büchs

  3. Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, Jülich, Deutschland

    Jörg Pietruszka

Authors
  1. Peter M. Kusen
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  2. Kyra Hoffmann
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  3. Georg Wandrey
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  4. Jochen Büchs
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  5. Jörg Pietruszka
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Corresponding author

Correspondence to Peter M. Kusen.

Additional information

Peter M. Kusen 2008–2013 Biochemiestudium (M. Sc.) an der Universität Düsseldorf. Seit 2014 Promotion am Institut für Bioorganische Chemie (Prof. Dr. J. Pietruszka) der Universität Düsseldorf am Forschungszentrum Jülich.

Kyra Hoffmann 2009–2015 Biotechnologiestudium (M. Sc.) an der RWTH Aachen. Seit 2015 Promotion am Lehrstuhl für Bioverfahrenstechnik der RWTH Aachen.

Georg Wandrey 2006–2012 Verfahrenstechnikstudium (Dipl.-Ing.) an der Universität Erlangen-Nürnberg. 2009–2012 Biochemical-Engineering-Studium (M. Sc.) an der Dongseo University Busan, Südkorea. Seit 2013 Promotion am Lehrstuhl für Bioverfahrenstechnik der RWTH Aachen.

Jochen Büchs 1975–1981 Verfahrenstechnikstudium an der TU München. 1982–1984 Forschungsaufenthalt an der Tokyo Universität und RIKEN, Japan. 1984–1988 Promotion in der Forschungsanlage Jülich. 1988–1996 Tätigkeit in der Bioverfahrenstechnik der BASF AG und anschließend Leiter des Biotechnikums. Seit 1996 Professor für Bioverfahrenstechnik an der RWTH Aachen.

Jörg Pietruszka 1985–1991 Chemiestudium an der Universität Hamburg. 1993 Promotion an der Universität Hamburg (Prof. Dr. W. A. König). 1993–1995 Postdoktoranden-Aufenthalt an der Universität Cambridge (Prof. Dr. S. V. Ley). 2001 Habilitation an der Universität Stuttgart. Seit 2004 Professor an der Universität Düsseldorf und Leiter des Instituts für Bioorganische Chemie. Seit 2013 Direktor des IBG-1: Biotechnologie am Forschungszentrum Jülich.

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Kusen, P.M., Hoffmann, K., Wandrey, G. et al. Optische Genregulation in Mikrobioreaktoren. Biospektrum 23, 643–645 (2017). https://doi.org/10.1007/s12268-017-0850-0

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  • DOI: https://doi.org/10.1007/s12268-017-0850-0

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