Abstract
A number of assay techniques are currently used to study ligand binding to G protein-coupled receptors (GPCRs) in living cells. In contrast to FRET, BRET does only need the addition of a suitable substrate to generate light/energy which then excites the fluorescent binding partner. This article describes the newly developed NanoBRETTM method for monitoring ligand binding to GPCRs in live cells using a microplate reader. Saturation and competition binding experiments are shown.
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Maurer, F. NanoBRET – ein neues Nachweisverfahren für die GPCR-Ligandenbindung. Biospektrum 22, 398–399 (2016). https://doi.org/10.1007/s12268-016-0705-0
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DOI: https://doi.org/10.1007/s12268-016-0705-0