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Tirofiban Positively Regulates β1 Integrin and Favours Endothelial Cell Growth on Polylactic Acid Biopolymer Vascular Scaffold (BVS)

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Abstract

An unexpectedly high incidence of thrombosis in patients that received the polylactic acid bioresorbable vascular scaffold (BVS) suggests a delayed/incomplete endothelial repair with this stent. The anti-platelet agent tirofiban stimulates endothelial cell migration and proliferation, mediated by VEGF production. We investigated the tirofiban effect on the migration and adhesion of endothelial cells to BVS, in vitro. We performed human umbilical endothelial cell (HUVEC) cultures in the presence of BVS. Tirofiban, similarly to VEGF, increased the ability of HUVEC to grow on the vascular scaffold, compared to unstimulated or abciximab-treated cells. Tirofiban increased HUVEC expression of β1 and β3 integrins along with collagen and fibronectin. A role for β1 integrin in the “pro-adhesive and -migratory” signals elicited by tirofiban was suggested by use of an anti-β1-blocking antibody that prevented poly-levo-lactic acid vascular scaffold colonization. Our study suggests that tirofiban may improve the outcomes of patients receiving BVS by accelerating stent endothelization.

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Funding

We thank Cardiovascular Service for supporting our research.

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Authors and Affiliations

  1. Unità Operativa di Interventistica Cardiovascolare, Presidio Ospedaliero Pineta Grande, Castel Volturno, Italy

    Arturo Giordano, Nicola Corcione, Paolo Ferraro & Stefano Messina

  2. Unità Operativa di Emodinamica, Casa di Salute Santa Lucia, San Giuseppe Vesuviano, Naples, Italy

    Arturo Giordano, Nicola Corcione, Paolo Ferraro & Stefano Messina

  3. Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 5, 80131, Naples, Italy

    Simona Romano & Maria Fiammetta Romano

  4. Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Latina, Italy

    Giacomo Frati & Giuseppe Biondi Zoccai

  5. Department of AngioCardioNeurology, IRCCS Neuromed, Pozzilli, Italy

    Giuseppe Biondi Zoccai

  6. Centro Medico Laboratorio Milano, Milan, Italy

    Stefano Ottolini

Authors
  1. Arturo Giordano
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  2. Simona Romano
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  7. Stefano Messina
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  8. Stefano Ottolini
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  9. Maria Fiammetta Romano
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Corresponding author

Correspondence to Maria Fiammetta Romano.

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Conflict of Interest

Arturo Giordano is a consultant for Medtronic and Abbot; Simona Romano declares that she has no conflict of interest; Nicola Corcione declares that he has no conflict of interest; Giacomo Frati declares that he has no conflict of interest; Giuseppe Biondi Zoccai has consulted for Bayer and Abbot Vascular; Paolo Ferraro and Stefano Messina declares that they have no conflict of interest; Stefano Ottolini is a consultant for Correvio Italia, Cardiome Sarl; Maria Fiammetta Romano declares that she has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Associate Editor Adrian Chester oversaw the review of this article

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Giordano, A., Romano, S., Corcione, N. et al. Tirofiban Positively Regulates β1 Integrin and Favours Endothelial Cell Growth on Polylactic Acid Biopolymer Vascular Scaffold (BVS). J. of Cardiovasc. Trans. Res. 11, 201–209 (2018). https://doi.org/10.1007/s12265-018-9805-1

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  • DOI: https://doi.org/10.1007/s12265-018-9805-1

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