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Effect of Splenectomy to Short Bowel Syndrome in Rats

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Abstract

The aim of this study was to determine the effect of splenectomy in the short bowel syndrome. Twenty-four Wistar-albino rats weighing between 210 and 375 g were used. They were divided into three groups. In group A, short bowel syndrome (SBS) was created by 75 % bowel resection. In group B, SBS and splenectomy was performed. In group C, after transecting the bowel, it was anastomosed. Before and 45 days after the procedures, all rats were weighed. In all three groups, the first and final weight of the rats, the final bowel weight and length, the ileal and jejunal crypt depths, the villus height, the luminal diameter, the bowel wall thickness, and the number of apoptotic cells and mitosis per 100 crypt cell were compared. Periportal fibrosis, infiltration, bile stasis, and bile duct proliferation were detected in liver samples. The rat intestinal length and weight was the least in group B while the jejunal crypt depth was higher in group B than in group A and it was exactly the opposite for the jejunal and ileal villus heights. The ileal and jejunal luminal diameter, the ileal bowel wall thickness, the jejunal and ileal apoptotic cell number, the jejunal mitosis, and the periportal fibrosis were highest in group B. Adding splenectomy to an SBS model has a negative impact on bowel adaptation.

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References

  1. Batra A, Beattie RM (2013) Management of short bowel syndrome in infancy. Early Hum Dev 89(11):899–904

    Article  CAS  PubMed  Google Scholar 

  2. Tappenden KA (2014) Intestinal adaptation following resection. JPEN J Parenter Enteral Nutr 38(1 Suppl):23S–31S

    Article  PubMed  Google Scholar 

  3. Uko V, Radhakrishnan K, Alkhouri N (2012) Short bowel syndrome in children: current and potential therapies. Paediatr Drugs 14(3):179–188

    Article  PubMed  Google Scholar 

  4. Jeppesen PB (2013) Teduglutide for the treatment of short bowel syndrome. Drugs Today (Barc) 49(10):599–614

    CAS  Google Scholar 

  5. Shaw D, Gohil K, Basson MD (2012) Intestinal mucosal atrophy and adaptation. World J Gastroenterol 18(44):6357–6375

    Article  PubMed  PubMed Central  Google Scholar 

  6. Di Sabatino A, Carsetti R, Corazza GR (2011) Post-splenectomy and hyposplenic states. Lancet 378(9785):86–97

    Article  PubMed  Google Scholar 

  7. Tarantino G, Savastano S, Capone D, Colao A (2011) Spleen: a new role for an old player? World J Gastroenterol 17(33):3776–3784

    Article  PubMed  PubMed Central  Google Scholar 

  8. Zoli G, Corazza GR, Wood S, Bartoli R, Gasbarrini G, Farthing MJ (1998) Impaired splenic function and tuftsin deficiency in patients with intestinal failure on long term intravenous nutrition. Gut 43(6):759–762

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Sukhotnik I, Yakirevich E, Coran AG, Siplovich L, Krausz M, Hirsh M, Sabo E, Shiloni E (2002) Effect of transforming growth factor-alpha on intestinal adaptation in a rat model of short bowel syndrome. J Surg Res 108(2):235–242

    Article  CAS  PubMed  Google Scholar 

  10. Wang L, Tang Y, Rubin DC, Levin MS (2007) Chronically administered retinoic acid has trophic effects in the rat small intestine and promotes adaptation in a resection model of short bowel syndrome. Am J Physiol Gastrointest Liver Physiol 293(2):G517–G518

    Article  CAS  Google Scholar 

  11. Warner BW (2006) Short bowel syndrome. In: Grosfel JL, O’Neill JA Jr, Fonkalsrud EW, Coran AG (eds) Pediatric Surgery. Mosby, Philadelphia, pp 1369–1382

    Chapter  Google Scholar 

  12. Jaksic T, Gutierrez IM, Kang KH (2012) Short bowel syndrome. In: Coran AG, Adzick NS, Krummel TM, Laberge JM, Shamberger RC, Caldamone AA (eds) Pediatric Surgery. Elsevier-Saunders, Philadelphia, pp 1135–1145

    Chapter  Google Scholar 

  13. Di Sabatino A, Rosado MM, Ciccocioppo R, Cazzola P, Morera R, Corazza GR, Carsetti R (2005) Depletion of immunoglobulin M memory B cells is associated with splenic hypofunction in inflammatory bowel disease. Am J Gastroenterol 100(8):1788–1795

    Article  PubMed  Google Scholar 

  14. Ryu S, Kodama S, Ryu K, Schoenfeld DA, Faustman DL (2001) Reversal of established autoimmune diabetes by restoration of endogenous beta cell function. J Clin Invest 108(1):63–72

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Kodama S, Kühtreiber W, Fujimura S, Dale EA, Faustman DL (2003) Islet regeneration during the reversal of autoimmune diabetes in NOD mice. Science 302(5648):1223–1227

    Article  CAS  PubMed  Google Scholar 

  16. Chong AS, Shen J, Tao J, Yin D, Kuznetsov A, Hara M, Philipson LH (2006) Reversal of diabetes in non-obese diabetic mice without spleen cell-derived beta cell regeneration. Science 311(5768):1774–1775

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

Karabulut B planned and did the experiment and wrote the paper. Karaman NA contributed to this paper. Caydere M evaluated the pathology. Karabulut R is the guarantor and contributed to the experiment and wrote the paper.

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Authors and Affiliations

  1. Department of Pediatric Surgery, Ankara Training and Research Hospital, Gezegen Sokak 1/10, Gaziosmanpasa 06670, Cankaya, Ankara, Turkey

    Bilge Karabulut & Nihan Ayyildiz Karaman

  2. Department of Pathology, Ankara Training and Research Hospital, Ankara, Turkey

    Muzaffer Caydere

  3. Department of Pediatric Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    Ramazan Karabulut

Authors
  1. Bilge Karabulut
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  2. Nihan Ayyildiz Karaman
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  3. Muzaffer Caydere
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  4. Ramazan Karabulut
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Correspondence to Bilge Karabulut.

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Karabulut, B., Karaman, N.A., Caydere, M. et al. Effect of Splenectomy to Short Bowel Syndrome in Rats. Indian J Surg 79, 201–205 (2017). https://doi.org/10.1007/s12262-016-1465-2

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  • DOI: https://doi.org/10.1007/s12262-016-1465-2

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