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The Inhibitory Functions of Sparstolonin B against Ambient Fine Particulate Matter Induced Lung Injury

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Abstract

Particulate matter, with an aerodynamic diameter equal to or less than 2.5 µm (PM2.5), is an air pollutant that causes serious pulmonary injury when inhaled. Sparstolonin B (SsnB) from the Chinese herb, Sparganium stoloniferum inhibits the expression of inflammatory cytokines and is involved in survival pathways. We investigated the protective effects of SsnB against PM2.5-induced lung damage. PM2.5 was pretreated intranasally and 30 minutes later, SsnB was injected via a vein in the tail of mouse. The effects of SsnB on PM2.5-induced lung damages, barrier disruptive responses, and pulmonary inflammation and the underlying mechanism of SsnB were investigated. SsnB significantly reduced pathological lung injury, the lung wet/dry weight ratio, and the levels of permeability. It also considerably attenuated PM2.5-induced myeloperoxidase activity in lung tissue, reduced PM2.5-induced inflammatory cytokine levels, and suppressed PM2.5-induced lymphocytes in bronchial alveolar fluid. Moreover, SsnB increased the phosphorylation of the mammalian target of rapamycin (mTOR) and significantly inhibited the expression of PM2.5-stimulated toll-like receptor 2, 4 (TLR2, 4), MyD88, and autophagy-related proteins, LC3II and Beclin 1. We concluded that SsnB regulates both the TLR2, 4-MyD88 and mTOR-autophagy pathways, therefore SsnB can be used as a potential therapeutic agent for preventing PM2.5-induced pulmonary damage.

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Acknowledgements

This study was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI15C0001) and by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2020R1A2C1004131 and 2022R1A4A10189001).

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Authors and Affiliations

  1. College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea

    Chaeyeong Kim, Soo Ho Ryu & Jong-Sup Bae

  2. College of Pharmacy, Yeungnam University, Gyeongsan, 38541, Korea

    Hyukjae Choi

  3. Department of Ophthalmology, School of Medicine, Kyungpook National University, Kyungpook National University Hospital, Daegu, 41944, Korea

    Dong Ho Park

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  1. Chaeyeong Kim
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Kim, C., Ryu, S.H., Choi, H. et al. The Inhibitory Functions of Sparstolonin B against Ambient Fine Particulate Matter Induced Lung Injury. Biotechnol Bioproc E 27, 949–960 (2022). https://doi.org/10.1007/s12257-022-0286-3

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