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Aptamer-immobilized Gold Nanoparticles Enable Facile and On-site Detection of Staphylococcus aureus

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Abstract

Staphylococcus aureus causes numerous illnesses and national loss in many countries. Therefore, to prevent food poisoning outbreaks, a rapid and straightforward on-site detection method is required without special skills or equipment. Here, we used aptamer-immobilized gold nanoparticles (aiGNPs) for the on-site colorimetric S. aureus detection. For the rapid and facile detection of S. aureus, the SA61 aptamer hybridized with thiol-modified adaptors conjugated onto the GNP surface of approximately 13 nm diameter. The synthesis of GNPs was confirmed using a transmission electron microscopy and energy dispersive spectroscopy. Images using a scanning electron microscope showed attachment of aiGNPs to S. aureus. After 30-min incubation and 20-min centrifugation, the red pellet was specifically observed with the sensitivity of OD600 0.050 equivalent to 7.5 × 106 cells/mL. Besides, aiGNPs could accurately detect S. aureus with a detection limit of OD600 0.10 equivalent to 1.5 × 107 cells/mL even in the mixed sample with Escherichia coli. Quantitative analysis was performed between relative area and cell concentration. Because this method enables fast and specific S. aureus detection without special skills or expensive equipment, aiGNPs could enable a rapid and effective on-site detection of S. aureus to prevent pathogenic outbreaks.

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Acknowledgments

An Incheon National University Research Grant supported this research in 2017.

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  1. Equal contribution

Authors and Affiliations

  1. Department of Chemistry, Incheon National University, Incheon, 22012, Korea

    Seong Ha Lim

  2. Department of Bioengineering and Nano-bioengineering, Incheon National University, Incheon, 22012, Korea

    Yeong Chae Ryu & Byeong Hee Hwang

  3. Division of Bioengineering, Incheon National University, Incheon, 22012, Korea

    Seong Ha Lim & Byeong Hee Hwang

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  1. Seong Ha Lim
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  2. Yeong Chae Ryu
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Correspondence to Byeong Hee Hwang.

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Lim, S.H., Ryu, Y.C. & Hwang, B.H. Aptamer-immobilized Gold Nanoparticles Enable Facile and On-site Detection of Staphylococcus aureus. Biotechnol Bioproc E 26, 107–113 (2021). https://doi.org/10.1007/s12257-020-0161-z

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  • DOI: https://doi.org/10.1007/s12257-020-0161-z

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