Abstract
Staphylococcus aureus is one of the ubiquitous bacterial pathogens in human that are able to cause various diseases from skin infections to serious diseases. This pathogen can make seven types of bacterial toxins that are accountable for food poisoning. Moreover, the pandemic of methicillin-resistant Staphylococcus aureus has been globally dangerous for chronically infectious diseases, and nosocomial infections with life-threatening problems, because of their resistance to most antibiotics. Therefore, rapid and accurate detection of S. aureus has emerged as an urgent need in fields of clinical diagnostics, biodefense and food safety. However, the current rapid tests are challenging with being limited their capacities in on-site detection for the increase of prevention results. In this study, we report an aptamer-based sensor for fast detection of S. aureus. Instead of trying to capture bacterial cells on the substrate or nanoparticle surface, our detection method allows the DNA aptamer to detach from the silver nanoparticle when interacting with bacteria and bind to its target, leading to the aggregation of silver nanoparticle by salt. The strategy dramatically enhances the selectivity and sensitivity of whole-cell based biosensor. The results demonstrated that aptasensor could detect S. aureus with a detection limit of ~ 105 CFU/mL within 20 min. The detection method showed many advantages compared with conventional methods with respect to cost, simplicity and analysis time. This biosensor can be used as a powerful bio-analytical tool for whole-cell detection.
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Acknowledgements
This research is funded by Vietnam National University Ho Chi Minh City (VNU-HCM) under grant number NCM2020-28-01.
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Truong, P.L. (2022). Fast Detection of Staphylococcus aureus Using DNA Aptamer and Silver Nanoparticles. In: Van Toi, V., Nguyen, TH., Long, V.B., Huong, H.T.T. (eds) 8th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2020. IFMBE Proceedings, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-030-75506-5_29
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DOI: https://doi.org/10.1007/978-3-030-75506-5_29
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